Abstract
Previous studies found that miR-374a-5p was decreased in infants suffering from the hypoxic-ischemic encephalopathy (HIE) compared with healthy control infants. However, the molecular mechanism of miR-374a-5p in the development of HIE remained unknown. This study is aimed to investigate the potential molecular pathway for shedding light on the treatment of HIE. An in vitro ischemia model in PC12 cells was established by oxygen/glucose deprivation (OGD). Reverse-transcription quantitative polymerase chain reaction and western blot were used to determine the levels of related genes or proteins in the OGD model or cells obtained from infants with HIE. Flow cytometry was conducted to quantify the apoptosis level of PC12 cells after OGD treatment. The TargetScan prediction algorithm was used to identify the potentially functional targets of miR-374a-5p. A dual-luciferase reporter assay was adopted to elucidate the sequences of miR-374a-5p binding to the 3′-UTR of potential target-PTEN. miR-374a-5p was downregulated in cells derived from human newborns with HIE, rat model with HIE, and PC12 cells after the OGD treatment. Inhibition of miR-374a-5p increased the expression of apoptotic markers and the apoptosis percentage of PC12 cells induced by OGD treatment while overexpression rescued the apoptosis. Meanwhile, PTEN expression was increased and suppressed after miR-374a-5p silence or overexpression, respectively. Upregulation of PTEN reversed the inhibitory effect of apoptotic markers elevation and PC12 cells apoptosis by the overexpression of miR-374a-5p after OGD treatment. PI3K pathway was required for the apoptosis effect caused by PTEN overexpression. We found that overexpression of miR-374a-5p reduced cell apoptosis through inhibiting PTEN/PI3K pathway in PC12 cells treated by OGD.
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References
Andersen GB, Knudsen A, Hager H, Hansen LL, Tost J (2018) miRNA profiling identifies deregulated miRNAs associated with osteosarcoma development and time to metastasis in two large cohorts. Mol Oncol 12:114–131
Bu SY, Yu GH, Xu GX (2013) Expression of insulin-like growth factor 1 receptor in rat retina following optic nerve injury. Acta Ophthalmol 91:e427–e431
Chalhoub N, Baker SJ (2009) PTEN and the PI3-kinase pathway in cancer. Annu Rev Pathol 4:127–150
Dalmasso B, Hatse S, Brouwers B, Laenen A, Berben L, Kenis C, Smeets A, Neven P, Schoffski P, Wildiers H (2018) Age-related microRNAs in older breast cancer patients: biomarker potential and evolution during adjuvant chemotherapy. BMC Cancer 18:1014
Engelman JA, Luo J, Cantley LC (2006) The evolution of phosphatidylinositol 3-kinases as regulators of growth and metabolism. Nat Rev Genet 7:606–619
Fernandez-Lopez D, Martinez-Orgado J, Casanova I, Bonet B, Leza JC, Lorenzo P, Moro MA, Lizasoain I (2005) Immature rat brain slices exposed to oxygen-glucose deprivation as an in vitro model of neonatal hypoxic-ischemic encephalopathy. J Neurosci Methods 145:205–212
Freedman BS, Lam AQ, Sundsbak JL, Iatrino R, Su X, Koon SJ, Wu M, Daheron L, Harris PC, Zhou J, Bonventre JV (2013) Reduced ciliary polycystin-2 in induced pluripotent stem cells from polycystic kidney disease patients with PKD1 mutations. J Am Soc Nephrol 24:1571–1586
Garcia-Junco-Clemente P, Golshani P (2014) PTEN: a master regulator of neuronal structure, function, and plasticity. Commun Integr Biol 7:e28358
Guillermet-Guibert J, Bjorklof K, Salpekar A, Gonella C, Ramadani F, Bilancio A, Meek S, Smith AJ, Okkenhaug K, Vanhaesebroeck B (2008) The p110beta isoform of phosphoinositide 3-kinase signals downstream of G protein-coupled receptors and is functionally redundant with p110gamma. Proc Natl Acad Sci U S A 105:8292–8297
Han Y, Li H (2018) miRNAs as biomarkers and for the early detection of non-small cell lung cancer (NSCLC). J Thorac Dis 10:3119–3131
He XY, Liao YD, Guo XQ, Wang R, Xiao ZY, Wang YG (2016) Prognostic role of microRNA-21 expression in brain tumors: a meta-analysis. Mol Neurobiol 53:1856–1861
Kosaka N, Iguchi H, Ochiya T (2010) Circulating microRNA in body fluid: a new potential biomarker for cancer diagnosis and prognosis. Cancer Sci 101:2087–2092
Looney AM, Walsh BH, Moloney G, Grenham S, Fagan A, O'Keeffe GW, Clarke G, Cryan JF, Dinan TG, Boylan GB, Murray DM (2015) Downregulation of umbilical cord blood levels of miR-374a in neonatal hypoxic ischemic encephalopathy. J Pediatr 167:269–273.e262
Mumford, S.L., Towler, B.P., and Pashler, A.L. (2018). Circulating microRNA biomarkers in melanoma: tools and challenges in personalised medicine. 8
Nair, J., and Kumar, V.H.S. (2018). Current and emerging therapies in the management of hypoxic ischemic encephalopathy in neonates. 5
Nassar FJ, Nasr R, Talhouk R (2017) MicroRNAs as biomarkers for early breast cancer diagnosis, prognosis and therapy prediction. Pharmacol Ther 172:34–49
Park IH, Arora N, Huo H, Maherali N, Ahfeldt T, Shimamura A, Lensch MW, Cowan C, Hochedlinger K, Daley GQ (2008) Disease-specific induced pluripotent stem cells. Cell 134:877–886
Peng, F., Tang, H., Liu, P., Shen, J., and Guan, X. (2017). Isoliquiritigenin modulates miR-374a/PTEN/Akt axis to suppress breast cancer tumorigenesis and metastasis. 7, 9022
Pierrat V, Haouari N, Liska A, Thomas D, Subtil D, Truffert P (2005) Prevalence, causes, and outcome at 2 years of age of newborn encephalopathy: population based study. Arch Dis Child Fetal Neonatal Ed 90:F257–F261
Pulido R (2018) PTEN inhibition in human disease therapy. Molecules (Basel, Switzerland) 23
Qiu J, Zhou XY, Zhou XG, Cheng R, Liu HY, Li Y (2013) Neuroprotective effects of microRNA-210 against oxygen-glucose deprivation through inhibition of apoptosis in PC12 cells. Mol Med Rep 7:1955–1959
Schmid MC, Avraamides CJ, Dippold HC, Franco I, Foubert P, Ellies LG, Acevedo LM, Manglicmot JR, Song X, Wrasidlo W et al (2011) Receptor tyrosine kinases and TLR/IL1Rs unexpectedly activate myeloid cell PI3kgamma, a single convergent point promoting tumor inflammation and progression. Cancer Cell 19:715–727
Shalom-Feuerstein R, Serror L, Aberdam E, Muller FJ, van Bokhoven H, Wiman KG, Zhou H, Aberdam D, Petit I (2013) Impaired epithelial differentiation of induced pluripotent stem cells from ectodermal dysplasia-related patients is rescued by the small compound APR-246/PRIMA-1MET. Proc Natl Acad Sci U S A 110:2152–2156
Takadera T, Matsuda I, Ohyashiki T (1999) Apoptotic cell death and caspase-3 activation induced by N-methyl-D-aspartate receptor antagonists and their prevention by insulin-like growth factor I. J Neurochem 73:548–556
Wang, Z., and Liu, Y. (2018). Combined prediction of miR-210 and miR-374a for severity and prognosis of hypoxic-ischemic encephalopathy. 8, e00835
Yang X, Wu Y, Zhang B, Ni B (2018) Noncoding RNAs in multiple sclerosis, vol 10, p 149
Yuan Y, Zheng Z (2019) Geniposide protects PC-12 cells against oxygen and glucose deprivation-induced injury by up-regulation of long-noncoding RNA H19. Life Sci 216:176–182
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Jiang, F., Yang, M., Wu, C. et al. Potential Roles of miR-374a-5p in Mediating Neuroprotective Effects and Related Molecular Mechanism. J Mol Neurosci 69, 123–132 (2019). https://doi.org/10.1007/s12031-019-01340-w
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DOI: https://doi.org/10.1007/s12031-019-01340-w