Abstract
Age estimation constitutes one of the pillars of human identification. The auricular surface of the ilium presents as a durable and robust structure within the human skeletal framework, capable of enabling accurate age estimation in older adults. Amongst different documented auricular age estimation methods, the Buckberry-Chamberlain method offers greater objectivity through its component-based approach. The present study aimed to test the applicability of the Buckberry-Chamberlain method in an Indian population through a CT-based examination of the auricular surface. CT scans of 435 participants undergoing CT examinations following the advice of their treating physicians were scrutinized for different age-related auricular changes. Three of the five morphological features described by Buckberry-Chamberlain could be appreciated on CT scans, and thus further statistical analysis was restricted to these features. Transition analysis coupled with Bayesian inference was undertaken individually for each feature to enable age estimation from individual features, while circumventing age mimicry. A Bayesian analysis of individual features yielded highest accuracy percentages (98.64%) and error rates (12.99 years) with macroporosity. Transverse organization and apical changes yielded accuracy percentages of 91.67% and 94.84%, respectively, with inaccuracy computations of 10.18 years and 11.74 years, respectively. Summary age models, i.e. multivariate age estimation models, derived by taking this differential accuracy and inaccuracy into consideration yielded a reduced inaccuracy value of 8.52 years. While Bayesian analysis undertaken within the present study enables age estimation from individual morphological features, summary age models appropriately weigh all appreciable features to yield more accurate and reliable estimates of age.
Similar content being viewed by others
References
Calce SE. A New method to estimate adult age-at-death using the acetabulum. Am J Phys Anthropol. 2012;148(1):11–23. https://doi.org/10.1002/ajpa.22026.
Falys CG, Schutkowski H, Weston DA. Auricular surface aging: worse than expected? A test of the revised method on a documented historic skeletal assemblage. Am J Phys Anthropol. 2006;130(4):508–13. https://doi.org/10.1002/ajpa.20382.
Latham KE, Finnegan JM, Rhine S. Age estimation of the human skeleton [Internet]. Springfield, Ill.: Charles C. Thomas Publisher; 2010 [Accessed 16 Sep 2021]. Available from: http://public.ebookcentral.proquest.com/choice/publicfullrecord.aspx?p=592401.
De Tobel J, Fieuws S, Hillewig E, Phlypo I, van Wijk M, de Haas MB, et al. Multi-factorial age estimation: a Bayesian approach combining dental and skeletal magnetic resonance imaging. Forensic Sci Int. 2020; 306:110054. https://doi.org/10.1016/j.forsciint.2019.110054.
Lovejoy CO, Meindl RS, Mensforth RP, Barton TJ. Multifactorial determination of skeletal age at death: a method and blind tests of its accuracy. Am J Phys Anthropol. 1985;68(1):1–14. https://doi.org/10.1002/ajpa.1330680102.
Miranker M. A Comparison of Different Age Estimation Methods of the Adult Pelvis. J Forensic Sci. 2016;61(5):1173–9. https://doi.org/10.1111/1556-4029.13130.
Moraitis K, Zorba E, Eliopoulos C, Fox SC. A test of the revised auricular surface aging method on a modern European population. J Forensic Sci. 2014;59(1):188–94. https://doi.org/10.1111/1556-4029.12303.
Barrier P, Dedouit F, Braga J, Joffre F, Rougé D, Rousseau H, et al. Age at death estimation using multislice computed tomography reconstructions of the posterior pelvis. J Forensic Sci. 2009;54(4):773–8. https://doi.org/10.1111/j.1556-4029.2009.01074.x.
Buckberry JL, Chamberlain AT. Age estimation from the auricular surface of the ilium: a revised method. Am J Phys Anthropol. 2002;119(3):231–9. https://doi.org/10.1002/ajpa.10130.
Lovejoy CO, Meindl RS, Pryzbeck TR, Mensforth RP. Chronological metamorphosis of the auricular surface of the ilium: a new method for the determination of adult skeletal age at death. Am J Phys Anthropol. 1985;68(1):15–28. https://doi.org/10.1002/ajpa.1330680103.
Osborne DL, Simmons TL, Nawrocki SP. Reconsidering the auricular surface as an indicator of age at death. J Forensic Sci. 2004;49(5):1–7.
Igarashi Y, Uesu K, Wakebe T, Kanazawa E. New method for estimation of adult skeletal age at death from the morphology of the auricular surface of the ilium. Am J Phys Anthropol. 2005;128(2):324–39. https://doi.org/10.1002/ajpa.20081.
Hailey KJ. A comparative evaluation of auricular surface aging methods using the William M. Bass donated skeletal collection. Knoxville: The University of Tennessee; 2015.
Hens SM, Belcastro MG. Auricular surface aging: a blind test of the revised method on historic Italians from Sardinia. Forensic Sci Int. 2012;214(1–3):209.e1-5. https://doi.org/10.1016/j.forsciint.2011.07.043.
Merritt CE. Testing the accuracy of adult skeletal age estimation methods: original methods versus revised and newer methods. vis-à-vis: Explorations in Anthropology. 2013;12(1):102–119.
Mulhern DM, Jones EB. Test of revised method of age estimation from the auricular surface of the ilium. Am J Phys Anthropol. 2005;126(1):61–5. https://doi.org/10.1002/ajpa.10410.
Xanthopoulou P, Valakos E, Youlatos D, Nikita E. Assessing the accuracy of cranial and pelvic ageing methods on human skeletal remains from a modern Greek assemblage. Forensic Sci Int. 2018;286:266.e1-266.e8. https://doi.org/10.1016/j.forsciint.2018.03.005.
Flanaghan TP. Age estimation of the auricular surface of the ilium : a comparison between physical examination and photographic evidence. Sydney: Western Sydney University; 2019.
Pattamapaspong N, Kanthawang T, Singsuwan P, Sansiri W, Prasitwattanaseree S, Mahakkanukrauh P. Efficacy of three-dimensional cinematic rendering computed tomography images in visualizing features related to age estimation in pelvic bones. Forensic Sci Int. 2019;294:48–56. https://doi.org/10.1016/j.forsciint.2018.10.003.
Michopoulou E, Negre P, Nikita E, Kranioti EF. The auricular surface as age indicator in a modern Greek sample: a test of two qualitative methods. Forensic Sci Int. 2017;280:246.e1-246.e7. https://doi.org/10.1016/j.forsciint.2017.08.004.
Gocha TP, Ingvoldstad ME, Kolatorowicz A, Cosgriff-Hernandez MTJ, Sciulli PW. Testing the applicability of six macroscopic skeletal aging techniques on a modern Southeast Asian sample. Forensic Sci Int. 2015;249(318):e1-7. https://doi.org/10.1016/j.forsciint.2014.12.015.
Hens SM, Godde K. Auricular surface aging: comparing two methods that assess morphological change in the ilium with Bayesian analyses. J Forensic Sci. 2016;61(Suppl 1):S30-38. https://doi.org/10.1111/1556-4029.12982.
Jones M, Gordon G, Brits D. Age estimation accuracies from black South African os coxae. Homo. 2018;69(5):248–58. https://doi.org/10.1016/j.jchb.2018.08.004.
Winburn A. A comparison of pelvic age-estimation methods on two modern Iberian populations: bioarchaeological and forensic implications. New York: New York University; 2008.
Rivera-Sandoval J, Monsalve T, Cattaneo C. A test of four innominate bone age assessment methods in a modern skeletal collection from Medellin. Colombia Forensic Sci Int. 2018;282:232.e1-232.e8. https://doi.org/10.1016/j.forsciint.2017.11.003.
Storey R. An elusive paleodemography? A comparison of two methods for estimating the adult age distribution of deaths at late Classic Copan. Honduras Am J Phys Anthropol. 2007;132(1):40–7. https://doi.org/10.1002/ajpa.20502.
Nagaoka T, Jirata K. Demographic structure of skeletal populations in historic Japan: a new estimation of adult age-at-death distributions based on the auricular surface of the ilium. J Archaeol Sci. 2008;35(5):1370–7.
Rissech C, Wilson J, Winburn AP, Turbón D, Steadman D. A comparison of three established age estimation methods on an adult Spanish sample. Int J Legal Med. 2012;126(1):145–55.
San Millán M, Millán MS, Rissech C, Turbón D. A test of Suchey-Brooks (pubic symphysis) and Buckberry-Chamberlain (auricular surface) methods on an identified Spanish sample: paleodemographic implications. J Archaeol Sci. 2013;40(4):1743–51.
Villa C, Buckberry J, Cattaneo C, Lynnerup N. Technical note: reliability of Suchey-Brooks and Buckberry-Chamberlain methods on 3D visualizations from CT and laser scans. Am J Phys Anthropol. 2013;151(1):158–63.
Nikita E, Xanthopoulou P, Kranioti E. An evaluation of Bayesian age estimation using the auricular surface in modern Greek material. Forensic Sci Int. 2018;291:1–11. https://doi.org/10.1016/j.forsciint.2018.07.029.
Shedge R, Kanchan T, Garg PK, Dixit SG, Warrier V, Khera P, et al. Computed tomographic analysis of medial clavicular epiphyseal fusion for age estimation in Indian population. Leg Med (Tokyo). 2020;46:101735. https://doi.org/10.1016/j.legalmed.2020.101735.
Warrier V, Kanchan T, Garg PK, Dixit SG, Krishan K, Shedge R. CT-based evaluation of the acetabulum for age estimation in an Indian population. Int J Legal Med. 2022;136(3):785–95. https://doi.org/10.1007/s00414-021-02757-y.
Warrier V, Shedge R, Garg PK, Dixit SG, Krishan K, Kanchan T. Computed tomographic evaluation of the acetabulum for age estimation in an Indian population using principal component analysis and regression models. Int J Legal Med. 2022. https://doi.org/10.1007/s00414-022-02856-4.
Warrier V, Shedge R, Garg PK, Dixit SG, Krishan K, Kanchan T. Applicability of the Calce method for age estimation in an Indian population: a clinical CT-based study. Leg Med. 2022;59:102113. https://doi.org/10.1016/j.legalmed.2022.102113.
Wink AE. Pubic symphyseal age estimation from three-dimensional reconstructions of pelvic CT scans of live individuals. J Forensic Sci. 2014;59(3):696–702. https://doi.org/10.1111/1556-4029.12369.
Lottering N, Alston-Knox CL, MacGregor DM, Izatt MT, Grant CA, Adam CJ, et al. Apophyseal ossification of the iliac crest in forensic age estimation: computed tomography standards for modern Australian subadults. J Forensic Sci. 2017;62(2):292–307. https://doi.org/10.1111/1556-4029.13285.
Hall F, Forbes S, Rowbotham S, Blau S. Using PMCT of individuals of known age to test the Suchey-Brooks method of aging in Victoria. Australia J Forensic Sci. 2019;64(6):1782–7. https://doi.org/10.1111/1556-4029.14086.
Fliss B, Luethi M, Fuernstahl P, Christensen AM, Sibold K, Thali M, et al. CT-based sex estimation on human femora using statistical shape modeling. Am J Phys Anthropol. 2019;169(2):279–86. https://doi.org/10.1002/ajpa.23828.
Tunis TS, Sarig R, Cohen H, Medlej B, Peled N, May H. Sex estimation using computed tomography of the mandible. Int J Legal Med. 2017;131(6):1691–700. https://doi.org/10.1007/s00414-017-1554-1.
3D Slicer image computing platform [Internet]. 3D Slicer. [Accessed 27 Jan 2022]. Available from: https://slicer.org/.
Fedorov A, Beichel R, Kalpathy-Cramer J, Finet J, Fillion-Robin JC, Pujol S, et al. 3D Slicer as an image computing platform for the quantitative imaging network. Magn Reson Imaging. 2012;30(9):1323–41. https://doi.org/10.1016/j.mri.2012.05.001.
Konigsberg LW, Frankenberg SR. Bayes in biological anthropology. Am J Phys Anthropol. 2013;152(S57):153–84. https://doi.org/10.1002/ajpa.22397.
Lyle W. Konigsberg’s webpage [Internet]. [Accessed 26 Apr 2022]. Available from: http://faculty.las.illinois.edu/lylek/.
Getz SM. The use of transition analysis in skeletal age estimation. WIREs Forensic Science. 2020;2(6):e1378. https://doi.org/10.1002/wfs2.1378.
Nikita E, Nikitas P. Skeletal age-at-death estimation: Bayesian versus regression methods. Forensic Sci Int. 2019;297:56–64. https://doi.org/10.1016/j.forsciint.2018.07.029.
McHugh ML. Interrater reliability: the kappa statistic. Biochem Med (Zagreb). 2012;22(3):276–82.
Baccino E, Sinfield L, Colomb S, Baum TP, Martrille L. Technical note: the two step procedure (TSP) for the determination of age at death of adult human remains in forensic cases. Forensic Sci Int. 2014;244:247–51. https://doi.org/10.1016/j.forsciint.2014.09.005.
Konigsberg LW, Herrmann NP, Wescott DJ, Kimmerle EH. Estimation and evidence in forensic anthropology: age-at-death. J Forensic Sci. 2008;53(3):541–57.
Boldsen JL, Milner GR, Konigsberg LW, Wood JW. Transition analysis: a new method for estimating age from skeletons. In: Hoppa RD, Vaupel JW, Hoppa RD, Vaupel JW, editors. Paleodemography. Cambridge University Press; 2002. p. 73–106.
Kimmerle EH, Konigsberg LW, Jantz RL, Baraybar JP. Analysis of age-at-death estimation through the use of pubic symphyseal data. J Forensic Sci. 2008;53(3):558–68.
Konigsberg LW, Frankenberg SR. Deconstructing death in paleodemography. Am J Phys Anthropol. 2002;117(4):297–309. https://doi.org/10.1002/ajpa.10039.
Lucy D, Aykroyd RG, Pollard AM, Solheim T. A Bayesian approach to adult human age estimation from dental observations by Johanson’s age changes. J Forensic Sci. 1996;41(2):189–94.
Langley-Shirley N, Jantz RL. A Bayesian approach to age estimation in modern Americans from the clavicle. J Forensic Sci. 2010;55(3):571–83. https://doi.org/10.1111/j.1556-4029.2010.01089.x.
Bedford ME, Russell KF, Lovejoy CO, Meindl RS, Simpson SW, Stuart-Macadam PL. Test of the multifactorial aging method using skeletons with known ages-at-death from the Grant Collection. Am J Phys Anthropol. 1993;91(3):287–97. https://doi.org/10.1002/ajpa.1330910304.
Sashin D. A critical analysis of the anatomy and the pathologic changes of the sacro-iliac joints. J Bone Jt Surg. 1930;12(4):891–910.
Garvin HM, Passalacqua NV. Current practices by forensic anthropologists in adult skeletal age estimation. J Forensic Sci. 2012;57(2):427–33. https://doi.org/10.1111/j.1556-4029.2011.01979.x.
Mukaka M. A guide to appropriate use of correlation coefficient in medical research. Malawi Med J. 2012;24(3):69–71.
Applied statistics for the behavioral sciences (eBook, 2003) [WorldCat.org] [Internet]. [Accessed 2 Feb 2022]. Available from: https://www.worldcat.org/title/applied-statistics-for-the-behavioral-sciences/oclc/643936092.
Rissech C, Estabrook G, Cunha E, Malgosa A. Using the acetabulum to estimate age at death of adult males. J Forensic Sci. 2006;51:213–29. https://doi.org/10.1111/j.1556-4029.2006.00060.x.
Rougé-Maillart C, Vielle B, Jousset N, Chappard D, Telmon N, Cunha E. Development of a method to estimate skeletal age at death in adults using the acetabulum and the auricular surface on a Portuguese population. Forensic Sci Int. 2009;188(1–3):91–5. https://doi.org/10.1016/j.forsciint.2009.03.019.
Sullivan S, Flavel A, Franklin D. Age estimation in a sub-adult Western Australian population based on the analysis of the pelvic girdle and proximal femur. Forensic Sci Int. 2017;281:185.e1-185.e10. https://doi.org/10.1016/j.forsciint.2017.10.010.
Bailey C. Age-at-death Estimation: Accuracy and Reliability of Age-Reporting Strategies. Knoxville: The University of Tennessee; 2018. p. 2015.
Bascou A, Dubourg O, Telmon N, Dedouit F, Saint-Martin P, Savall F. Age estimation based on computed tomography exploration: a combined method. Int J Legal Med. 2021;135(6):2447–55. https://doi.org/10.1007/s00414-021-02666-0.
Cloven JM. Validation study of the proposed seventh phase of the Suchey-Brooks age estimation method for the pubic symphysis. 2015 [Accessed 18 Apr 2022]; Available from: https://open.bu.edu/handle/2144/15624.
Fleischman JM. A comparative assessment of the Chen et al. and Suchey-Brooks pubic aging methods on a North American sample. J Forensic Sci. 2013; 58(2):311–23. https://doi.org/10.1111/1556-4029.12061.
Hens SM, Godde K. A Bayesian approach to estimating age from the auricular surface of the ilium in modern American skeletal samples. Forensic Sci Int. 2022;2(4):682–95. https://doi.org/10.3390/forensicsci2040051.
Godde K, Hens SM. Age-at-death estimation in an Italian historical sample: a test of the Suchey-Brooks and transition analysis methods. Am J Phys Anthropol. 2012;149(2):259–65. https://doi.org/10.1002/ajpa.22126.
Carew R, Viner M, Conlogue G, Grant N, Beckett S. Accuracy of computed radiography in osteometry: a comparison of digital imaging techniques and the effect of magnification. J Forensic Radiol Imag. 2019;19:100348. https://doi.org/10.1016/j.jofri.2019.100348.
Ekizoglu O, Inci E, Erdil I, Hocaoglu E, Bilgili MG, Kazimoglu C, et al. Computed tomography evaluation of the iliac crest apophysis: age estimation in living individuals. Int J Legal Med. 2016;130(4):1101–7. https://doi.org/10.1007/s00414-016-1345-0.
Acknowledgements
This research article is a part of an ongoing doctoral research being conducted by the principal author in the Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, Jodhpur, India. The principal author is grateful to the University Grants Commission, New Delhi for awarding the research fellowship (UGC-JRF) for pursuing PhD. The authors are also thankful to the individuals who participated in this study, and to the authorities of the institution for allowing us to conduct this research.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Ethical approval
Approval was obtained from the Institutional Ethics Committee (Letter no. AIIMS/IEC/2019–20/1007) prior to commencement of the study.
Informed consent
All participants were informed about parameters of the study and CT images of consenting individuals were collected.
Research involving human participants and/or animals
Human participants.
Competing interests
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Warrier, V., Shedge, R., Garg, P.K. et al. Age estimation from iliac auricular surface using Bayesian inference and principal component analysis: a CT-based study in an Indian population. Forensic Sci Med Pathol (2023). https://doi.org/10.1007/s12024-023-00637-y
Accepted:
Published:
DOI: https://doi.org/10.1007/s12024-023-00637-y