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LncRNA MCM3AP-AS1 promotes proliferation and invasion through regulating miR-211-5p/SPARC axis in papillary thyroid cancer

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Abstract

Background

Long non-coding RNAs (lncRNAs) are an emerging class of regulators in cancer. A lncRNA, MCM3AP-AS1, has been demonstrated as a versatile mediator in many cancers, except papillary thyroid cancer. The aim of this study is to investigate the role and mechanism of MCM3AP-AS1 in papillary thyroid cancer.

Methods

Quantitative real-time PCR was used to assess the level of MCM3AP-AS1 and miR-211-5p in papillary thyroid cancer tissues and cells. Western blot was used to detect E-cadherin and secreted protein acidic and cysteine rich (SPARC) protein levels. CCK-8, scratch wound assay, and transwell assay were used to evaluate papillary thyroid cancer cell proliferation, migration, and invasion, respectively. BLAST alignment and luciferase assay were used to explore the interaction among MCM3AP-AS1, mi/r-211, and SPARC.

Results

In papillary thyroid cancer, MCM3AP-AS1 was upregulated, while miR-211 was downregulated. MCM3AP-AS1 overexpression promoted papillary thyroid cancer proliferation, migration, and invasion. Further, MCM3AP-AS1 was shown to be negatively correlated with miR-211-5p. We next validated that miR-211-5p overexpression could reverse the promoting role of MCM3AP-AS1 in papillary thyroid cancer, whereby SPARC plays an important regulating role. In vivo, we confirmed the anti-tumor role of MCM3AP-AS1 silencing and the close relation among MCM3AP-AS1, miR-211-5p, and SPARC.

Conclusions

MCM3AP-AS1 promotes papillary thyroid cancer by regulating the MCM3AP-AS1/miR-211-5p/SPARC axis, which could potentially be a therapeutic target in papillary thyroid cancer.

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Data availability

The analyzed data sets generated during the study are available from the corresponding author on reasonable request.

Abbreviations

PTC:

Papillary thyroid cancer

lncRNA:

Long non-coding RNA

miRNAs:

microRNAs

GECs:

glioma-associated endothelial cells

GBM:

Glioblastoma

GFP:

green fluorescent protein

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Author contributions

All authors have read and approved the final manuscript.

Funding

This work was supported by the Petrel Science Foundation of Harbin Medical University Cancer Hospital (Grant No. JJLX2014-01).

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Harbin Medical University, 150086, Harbin, Heilongjiang, People’s Republic of China

    Meihua Liang & Biyue Wei

  2. Department of Thyroid Surgery, Harbin Medical University Cancer Hospital, 150081, Harbin, Heilongjiang, People’s Republic of China

    Jinliang Jia, Lili Chen, Qiang Guan, Zhaoming Ding, Jiawei Yu, Rui Pang & Guoqing He

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  1. Meihua Liang
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Correspondence to Guoqing He.

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The present study was approved by the Ethics Committee of Harbin Medical University Cancer Hospital.

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All patients and healthy volunteers provided written informed consent prior to their inclusion within the study.

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Liang, M., Jia, J., Chen, L. et al. LncRNA MCM3AP-AS1 promotes proliferation and invasion through regulating miR-211-5p/SPARC axis in papillary thyroid cancer. Endocrine 65, 318–326 (2019). https://doi.org/10.1007/s12020-019-01939-4

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