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Effects of Dietary Zinc-Methionine Supplementation During Pregnancy on the Whole-Genome Methylation and Related Gene Expression in the Liver and Spleen of Growing Goats: a Short Communication

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Abstract

The effect of replacing inorganic zinc with organic zinc in diets of pregnant goats was investigated on the development of the liver and spleen of offspring. Pregnant goats (n = 14; Xiangdong black goat, local breed) of similar parity and body weight (BW, 37.17 ± 5.28 kg) were selected and divided randomly into two groups: the zinc sulfate group (ZnSO4; n = 7) and the methionine-chelated zinc group (Zn-Met; n = 7). Goats were fed for 45 days (day 106 of gestation to delivery). After delivering, lactating goats were fed a diet without extra zinc supplement. Kid goats were weaned at 2 months of age and both the groups were fed the same diet. All goats were fed a mixed diet and had free access to fresh water. Kid goats were slaughtered on day 100, and the liver and spleen were collected, weighed, and stored in liquid nitrogen for genomic DNA methylation and related gene expression determination. In the Zn-Met group, the liver organ index of kid goats showed an increasing trend (P < 0.10), but the methylation of the whole genome was not affected both in the liver and spleen (P > 0.10). Furthermore, the blood zinc content of the offspring was reduced (P < 0.05), and the expression of genes related to methylation were downregulated (P < 0.05) or showed a downward trend (P < 0.10) in the liver and spleen. These data indicated that goats feeding Zn-Met during pregnancy increased the offspring liver organ index without change in the genomic DNA methylation. It is speculated that the regulation of zinc finger protein Sp3 adjusted by blood zinc indirectly regulated the expression of methylation-related genes in the liver and spleen of the kid goats, thus enhancing the development and function of the immune system of the offspring.

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References

  1. Underwood EJ (1977) 8 - zinc. In: Underwood EJ (ed) Trace elements in human and animal nutrition, Fourth edn. Academic Press, Salt Lake City, pp 196–242. https://doi.org/10.1016/B978-0-12-709065-8.50012-2

    Chapter  Google Scholar 

  2. Apgar J, Travis HF (1979) Effect of a low zinc diet on the ewe during pregnancy and lactation. J Anim Sci 48(5):1234–1238. https://doi.org/10.2527/jas1979.4851234x

    Article  CAS  PubMed  Google Scholar 

  3. Masters DG, Moir RJ (1983) Effect of zinc-deficiency on the pregnant ewe and developing fetus. Br J Nutr 49(3):365–372. https://doi.org/10.1079/BJN19830045

    Article  CAS  PubMed  Google Scholar 

  4. Vallee BL, Falchuk KH (1993) The biochemical basis of zinc physiology. Physiol Rev 73(1):79–118. https://doi.org/10.1152/physrev.1993.73.1.79

    Article  CAS  PubMed  Google Scholar 

  5. Spears JW, Harvey RW, Brown TT (1991) Effects of zinc methionine and zinc oxide on performance, blood characteristics, and antibody titer response to viral vaccination in stressed feeder calves. J Am Vet Med Assoc 199(12):1731–1733

    CAS  PubMed  Google Scholar 

  6. Ashmead DW (1993) Comparative intestinal absorption and subsequent metabolism of metal amino acid chelates and inorganic metal salts. In: The roles of amino acid chelates in animal nutrition, New Jersey, pp 47–74

  7. Lowe JA, Wiseman J, Cole DJ (1994) Absorption and retention of zinc when administered as an amino-acid chelate in the dog. J Nutr 124(12 Suppl):2572S–2574S. https://doi.org/10.1093/jn/124.suppl_12.2572S

    Article  CAS  PubMed  Google Scholar 

  8. Lowe JA, Wiseman J (1998) A comparison of the bioavailability of three dietary zinc sources using four different physiologic parameters in dogs. J Nutr 128(12 Suppl):2809S–2811S. https://doi.org/10.1093/jn/128.12.2809S

    Article  CAS  PubMed  Google Scholar 

  9. Moore CL, Walker PM, Winter JR, Jones MA, Webb JW (1989) Zinc methionine supplementation for dairy cows. Transactions of the Illinois State Academy of Science 71:99–108

  10. Kegley EB, Silzell SA, Kreider DL, Galloway DL, Coffey KP, Hornsby JA, Hubbell DS (2001) The immune response and performance of calves supplemented with zinc from an organic and an inorganic source. Prof Anim Sci 2:33–38. https://doi.org/10.15232/S1080-7446(15)31593-X

    Article  Google Scholar 

  11. Nagalakshmi D, Dhanalakshmi K, Himabindu D (2009) Effect of dose and source of supplemental zinc on immune response and oxidative enzymes in lambs. Vet Res Commun 33(7):631–644. https://doi.org/10.1007/s11259-009-9212-9

    Article  CAS  PubMed  Google Scholar 

  12. Fan WH, Tang G, Zhao CM, Duan Y, Zhang R (2009) Metal accumulation and biomarker responses in Daphnia magna following cadmium and zinc exposure. Environ Toxicol Chem 28(2):305–310. https://doi.org/10.1897/07-639.1

    Article  CAS  PubMed  Google Scholar 

  13. Duerre JA, Wallwork JC (1986) Methionine metabolism in isolated perfused livers from rats fed on zinc-deficient and restricted diets. Br J Nutr 56(2):395–405. https://doi.org/10.1079/BJN19860120

    Article  CAS  PubMed  Google Scholar 

  14. AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Arlington

    Google Scholar 

  15. Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74:3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

    Article  Google Scholar 

  16. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402–408. https://doi.org/10.1006/meth.2001.1262

    Article  CAS  Google Scholar 

  17. Li X, Yan Q, Tang S, Tan Z, Fitzsimmons CJ, Yi K (2018) Effects of maternal feed intake restriction during pregnancy on the expression of growth regulation, imprinting and epigenetic transcription-related genes in foetal goats. Anim Reprod Sci 198:90–98. https://doi.org/10.1016/j.anireprosci.2018.09.005

    Article  CAS  PubMed  Google Scholar 

  18. Kloubert V, Blaabjerg K, Dalgaard TSR, Poulsen HD, Rink L, Wessels I (2018) Influence of zinc supplementation on immune parameters in weaned pigs. J Trace Elem Med Biol S0946672X17308787. https://doi.org/10.1016/j.jtemb.2018.01.006

  19. Ross CJER (1997) The somatogenic hormones and insulin-like growth factor-1: stimulators of lymphopoiesis and immune function. J Trace Elem Med Biol 2:2–179. https://doi.org/10.1210/edrv.18.2.0296

    Article  Google Scholar 

  20. Roselli M, Finamore A, Garaguso I, Britti MS, Mengheri E (2003) Zinc oxide protects cultured enterocytes from the damage induced by Escherichia coli. J Nutr 133(12):4077–4082. https://doi.org/10.1093/jn/133.12.4077

    Article  CAS  PubMed  Google Scholar 

  21. Chen YP, Cheng YF, Li XH, Zhang H, Yang WL, Wen C, Zhou YM (2016) Dietary palygorskite supplementation improves immunity, oxidative status, intestinal integrity, and barrier function of broilers at early age. Anim Feed Sci Technol 219:200–209. https://doi.org/10.1016/j.anifeedsci.2016.06.013

    Article  CAS  Google Scholar 

  22. Salgueiro MJ, Zubillaga M, Lysionek A, Sarabia MI, Caro R, Paoli TD, Hager A, Weill R (2000) Zinc as an essential micronutrient: a review. Nutr Res 20(5):737–755. https://doi.org/10.1016/S0271-5317(00)00163-9

    Article  CAS  Google Scholar 

  23. Wallwork JC, Duerre JA (1985) Effect of zinc deficiency on methionine metabolism, methylation reactions and protein synthesis in isolated perfused rat liver. J Nutr 2:2–262. https://doi.org/10.1093/jn/115.2.252

    Article  Google Scholar 

  24. Bechmann LP, Hannivoort RA, Gerken G, Hotamisligil GS, Trauner M, Canbay AJ (2012) The interaction of hepatic lipid and glucose metabolism in liver diseases. J Hepatol 56(4):952–964. https://doi.org/10.1016/j.jhep.2011.08.025

    Article  CAS  PubMed  Google Scholar 

  25. Yi L, Wang Y, Zhang N, Fan CY (2008) Effects of different concentrations of zinc methionine on growth performance and immune function of young pigeons. China Feed 6:23–25

    Google Scholar 

  26. Yu ZP (2005) Studies on growth, immune regulation and molecular mechanism of zinc and its source in animals. Ph.D., Jiangnan University

  27. Sandoval M, Henry PR, Luo XG, Littell RC, Miles RD, Ammerman CB (1998) Performance and tissue zinc and metallothionein accumulation in chicks fed a high dietary level of zinc. Poult Sci 77(9):1354–1363. https://doi.org/10.1093/ps/77.9.1354

    Article  CAS  PubMed  Google Scholar 

  28. Brandão-Neto J, Stefan V, Mendonca B, Bloise W, Castro A (1995) The essential role of zinc in growth. Nutr Res 15:335–358. https://doi.org/10.1016/0271-5317(95)00003-8

    Article  Google Scholar 

  29. Unterberger A, Szyf M, Nathanielsz PW, Cox LA (2009) Organ and gestational age effects of maternal nutrient restriction on global methylation in fetal baboons. J Med Primatol 38(4):219–227. https://doi.org/10.1111/j.1600-0684.2008.00320.x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Lindeman LC, Winata CL, Hvard A, Sinnakaruppan M, Peter A, Philippe C (2010) Chromatin states of developmentally-regulated genes revealed by DNA and histone methylation patterns in zebrafish embryos. Int J Dev Biol 54(5):803–813. https://doi.org/10.1387/ijdb.103081ll

    Article  CAS  PubMed  Google Scholar 

  31. Chrivia JC, Kwok RPS, Lamb N, Hagiwara M, Montminy MR, Goodman RH (1993) Phosphorylated creb binds specifically to the nuclear-protein cbp. Nature 365(6449):855–859. https://doi.org/10.1038/365855a0

    Article  CAS  PubMed  Google Scholar 

  32. Nan XS, Ng HH, Johnson CA, Laherty CD, Turner BM, Eisenman RN, Bird A (1998) Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature 393(6683):386–389. https://doi.org/10.1038/30764

    Article  CAS  PubMed  Google Scholar 

  33. Kishikawa S, Murata T, Kimura H, Shiota K, Yokoyama KK (2002) Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc finger proteins. Eur J Biochem 269(12):2961–2970. https://doi.org/10.1046/j.1432-1033.2002.02972.x

    Article  CAS  PubMed  Google Scholar 

  34. Jinawath A, Miyake S, Yanagisawa Y, Akiyama Y, Yuasa Y (2005) Transcriptional regulation of the human DNA methyltransferase 3A and 3B genes by Sp3 and Sp1 zinc finger proteins. Biochem J 385(Pt 2):557–564. https://doi.org/10.1042/BJ20040684

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Barreto G, Schafer A, Marhold J, Stach D, Swaminathan SK, Handa V, Doderlein G, Maltry N, Wu W, Lyko F, Niehrs C (2007) Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation. Nature 445(7128):671–675. https://doi.org/10.1038/nature05515

    Article  CAS  PubMed  Google Scholar 

  36. Kienhöfer S, Musheev MU, Stapf U, Helm M, Schomacher L, Niehrs C, Schäfer A (2015) GADD45a physically and functionally interacts with TET1. Differentiation 90(1–3):59–68. https://doi.org/10.1016/j.diff.2015.10.003

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Funding

This work was supported by the Youth Innovation Team Project of ISA, CAS (2017QXCXTD_ZCS), the National Natural Science Foundation of China (31730092, 31402105, and 31760678), and the State Key Project of Research and Development Plan (2018YFD0501604).

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Authors and Affiliations

  1. CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, 410125, Hunan, People’s Republic of China

    Qiushuang Li, Qiongxian Yan, Chuanshe Zhou, Shaoxun Tang, Xuefeng Han & Zhiliang Tan

  2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Qiushuang Li

  3. Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Changsha, 410128, People’s Republic of China

    Chuanshe Zhou, Shaoxun Tang, Xuefeng Han & Zhiliang Tan

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  1. Qiushuang Li
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  2. Qiongxian Yan
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Correspondence to Qiongxian Yan.

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All procedures performed in studies involving animals were in accordance with the Animal Care and Use Guidelines of the Animal Care Committee, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan Province, China (ethic number: ISACAS-02-2017-10).

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Li, Q., Yan, Q., Zhou, C. et al. Effects of Dietary Zinc-Methionine Supplementation During Pregnancy on the Whole-Genome Methylation and Related Gene Expression in the Liver and Spleen of Growing Goats: a Short Communication. Biol Trace Elem Res 199, 996–1001 (2021). https://doi.org/10.1007/s12011-020-02223-7

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