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Correlation Between Size and Activity Enhancement of Recombinantly Assembled Cellulosomes

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Abstract

As multienzyme complexes, cellulosomes hydrolyze cellulosic biomass with high efficiency, which is believed to be attributed to either one or both factors: (1) synergy among the catalytic and substrate-binding entities and (2) the large size of cellulosome complexes. Although the former factor has been extensively documented, the correlation between size and specific activity of cellulosomes is still elusive to date. In this study, primary and secondary scaffoldins with 1, 3, or 5 copies of type I/II cohesin domains were recombinantly synthesized and various cellulosomes carrying 1, 3, 5, 9, 15, or 25 molecules of cellulase mixtures of family 5, 9, and 48 glycoside hydrolases were assembled. In addition, the assembled complex was annexed to cellulose with the aid of a family 3a carbohydrate-binding module (CBM3a). Measuring cellulolytic hydrolysis activities of assembled cellulosomes on crystalline Avicel revealed that higher degree of cellulosome complexity resulted in more efficient cellulose hydrolysis with plateaued synergic effects after the cellulosome size reaches certain degree.

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Funding

This research was supported by National Science Foundation (CBET 1265044).

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  1. Department of Chemical and Environmental Engineering, University of California Riverside, 900 University Ave, Riverside, CA, 92512, USA

    Long Chen & Xin Ge

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  1. Long Chen
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Correspondence to Xin Ge.

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Chen, L., Ge, X. Correlation Between Size and Activity Enhancement of Recombinantly Assembled Cellulosomes. Appl Biochem Biotechnol 186, 937–948 (2018). https://doi.org/10.1007/s12010-018-2786-3

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  • DOI: https://doi.org/10.1007/s12010-018-2786-3

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