Abstract
For the first time, interaction between non-toxic anthraquinone morindone with both natural and synthetic DNA duplexes has been demonstrated in this paper. Detailed analyses of the binding of morindone with DNA via UV–vis, FTIR, and circular dichroism spectroscopies were carried out. In addition, bioinformatics tools have been employed to scrutinize the binding of the dye with DNA in silico. Results represent morindone to be a better binder with a score of −5.79 compared to EtBr (known mutagenic intercalator) recorded at −5.02. Further interaction is accentuated by the microscope-assisted evidence of nuclear specific staining of tissues by morindone. The electrophoretic analysis reveals the efficacy endowed within morindone dye in rendering protection to DNA exposed to H2O2 damage and thereby conferring it safe to the nucleic acid. As DNA is often the target for majority of anticancer and antibiotic drugs, study on the interaction between molecules like morindone and DNA has relevance and implications in several biological applications including cancer therapy. Thus, we propose that morindone can also be harnessed as a diagnostic probe for DNA structure in addition to DNA-directed therapeutics.
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Tan, J. H., Lu, Y., Huang, Z. S., Gu, L. Q., & Wu, J. Y. (2007). Spectroscopic studies of DNA binding modes of cation-substituted anthrapyrazoles derived from emodin. European Journal of Medicinal Chemistry, 42, 1169–1175.
Brana, M. F., Cacho, M., Gradillas, B., DePascual-Teresa, B., & Ramos, A. (2001). Intercalators as anticancer drugs. Current Pharmaceutical Design, 7, 1745–1780.
Neidle, S., Pearl, L. H., & Skelly, J. V. (1987). DNA structure and perturbation by drug binding. Biochemical Journal, 2143, 1–13.
Tan, W. B., Bhambhani, A., Duff, M. R., Rodger, A., & Kumar, C. V. (2006). Spectroscopic identification of binding modes of anthracene probes and DNA sequence recognition. Photochemistry and Photobiology, 82, 20–30.
Bi, S., Zhang, H., Qiao, C., Sunc, Y., & Liu, C. (2008). Studies of interaction of emodin and DNA in the presence of ethidium bromide by spectroscopic method. Spectrochimica Acta A, 69, 123–129.
Paul, P., Hossain, M., Yadav, R. C., & Kumar, G. S. (2010). Biophysical studies on the base specificity and energetics of the DNA interaction of photoactive dye thionine: spectroscopic and calorimetric approach. Biophysical Chemistry, 148, 93–103.
Lown, J. W., & Hanstock, C. C. (1985). High filed 1H-NMR analysis of the 1:1 intercalation complex of the anti tumor agent mitoxantrone and the DNA duplex [d(CpGpCpG)]. Journal of Biomolecular Structure and Dynamics, 2, 1097–1106.
Siva, R. (2007). Status of natural dyes and dye yielding plants in India. Current Science, 92, 916–925.
Anekpankul, T., Goto, M., Sasaki, M., Pavasant, P., & Shotipruk, A. (2007). Extraction of anti-cancer damnacanthal from roots of Morinda citrifolia by subcritical water. Separation and Purification Technology, 55, 343–349.
Alagesaboopathi, C. (2009). Ethnomedicinal plants and their utilization by villagers in Kumaragiri Hills of Salem District of Tamil Nadu, India. African Journal of Traditional Complementary and Alternative Medicine, 6, 222–227.
Vijayalakshmi, R., Kanthimanthi, M., & Subramanian, V. (2000). DNA cleavage by a Chromium(III) complex. Biochemical and Biophysical Research Communications, 271, 731–734.
Aobchey, P., Sriyam, S., Praharnripoorab, W., Lhieochaiphant, S., & Phutrakul, S. (2002). Production of red pigment from the root of Morinda angustifolia Roxb. var. scabridula Craib. by root cell culture. CMU Journal, 1, 66–78.
Russo, A., Izzo, A. A., Cardile, V., Borrelli, F., & Vanella, A. (2001). Indian medicinal plants as antiradicals and DNA cleavage protector. Phytomedicine, 8, 125–132.
Vaidyanathan, V. G., & Nair, B. U. (2005). Synthesis, characterization and DNA-binding studies of mixed ligand complexes of ruthenium(II) with DNA. Journal of Chemical Society Dalton Transactions, 17, 2842–2848.
Cate, J. H., & Doudna, J. A. (1996). Metal binding sites in the major groove of a large ribozyme domain. Structure, 4, 1221–1229.
Zhang, L. Z., & Tang, G. Q. (2004). The binding properties of photosensitizer methylene blue to herring sperm DNA: a spectroscopic study. Journal of Photochemistry and Photobiology B: Biology, 74, 119–125.
Long, E. C., & Barton, J. K. (1990). On demonstrating DNA intercalation. Accounts of Chemical Research, 23, 271–273.
Mao, Y., Daniel, L. N., Whittaker, N., & Saffiottil, U. (1994). DNA binding to crystalline silica characterized by Fourier-transform infrared spectroscopy. Environmental Health Perspectives, 102, 65–171.
Hackl, E. V., Kornilova, S. V., Kapinos, L. E., Andrushchenko, V. V., Galkin, V. L., Grigoriev, D. N., et al. (1997). Study of Ca+2, Mn+2 and Cu+2 binding to DNA in solution by means of IR spectroscopy. Journal of Molecular Structure, 408(409), 229–232.
Zunino, F., Animati, F., & Capranico, C. (1995). DNA minor-groove binding drugs. Current Pharmaceutical Design, 1, 83–94.
Rajendra, T. K., Gonsalvez, G. B., Walker, M. P., Shpargel, K. B., Salz, H. K., & Matera, A. G. (2007). A Drosophila melanogaster model of spinal muscular atrophy reveals a function for SMN in striated muscle. The Journal of Cell Biology, 176, 831–841.
Cremazy, F. G. E., Manders, E. M. M., Bastiaens, P. I. H., Kramer, G., Hager, G. L., van Munster, E. B., et al. (2005). Imaging in situ protein–DNA interactions in the cell nucleus using FRET–FLIM. Experimental Cell Research, 309, 390–396.
Dutt, M. K. (1982). Basic dyes for the staining of DNA in mammalian tissues and absorption spectra of stained nuclei in the visible light. Microscopica Acta, 86, 59–68.
Lu, W., Vicic, D. A., & Barton, J. K. (2005). Reductive and oxidative DNA damage by photoactive platinum(II) intercalators. Inorganic Chemistry, 44, 7970–7980.
Faddeeva, M. D., & Beliaeva, T. N. (1991). DNA intercalators: their interaction with DNA and other cell components and their use in biological research. Tsitologiia, 33, 3–31.
Ferguson, L. R., & Denny, W. A. (2007). Genotoxicity of non-covalent interactions: DNA Intercalators. Mutation Research, 623, 14–23.
Acknowledgments
We express our sincere gratitude to the support extended by CSIR, New Delhi, India through the means of the sanctioned project [37(1451)/10/EMR-II]. The authors are grateful to VIT University management for their constant support. We thank Professor G. Jayaraman for his critical suggestions regarding the CD analysis. Thanks to Dr. A. Ramakrishna for chemical characterization of morindone. The authors thank Dr. Anand Prem Rajan for his immaculate help in the histological analyses.
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The authors declare that there is no conflict of interest.
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Bhakta, D., Siva, R. Morindone, an Anthraquinone, Intercalates DNA Sans Toxicity: a Spectroscopic and Molecular Modeling Perspective. Appl Biochem Biotechnol 167, 885–896 (2012). https://doi.org/10.1007/s12010-012-9744-2
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DOI: https://doi.org/10.1007/s12010-012-9744-2