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Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

  • Symposium: Bone Repair and Regeneration
  • Published:
Clinical Orthopaedics and Related Research®

Abstract

Background

Periosteal cells are important in embryogenesis, fracture healing, and cartilage repair and could provide cells for osteochondral tissue engineering.

Questions/purpose

We determined whether a population of cells isolated from human periosteal tissue contains cells with a mesenchymal stem cell (MSC) phenotype and whether these cells can be expanded in culture and used to form tissue in vitro.

Methods

We obtained periosteal tissue from six patients. Initial expression of cell surface markers was assessed using flow cytometry. Cells were cultured over 10 generations and changes in gene expression evaluated to assess phenotypic stability. Phenotype was confirmed using flow cytometry and colony-forming ability assays. Mineral formation was assessed by culturing Stro-1− and unsorted cells with osteogenic supplements. Three cell culture samples were used for a reverse transcription–polymerase chain reaction, four for flow cytometry, three for colony-forming assay, and three for mineralization.

Results

Primary cultures, containing large numbers of hematopoietic cells were replaced initially by Stro-1 and ALP-expressing immature osteoblastic cell types and later by ALP-expressing cells, which lacked Stro-1 and which became the predominant cell population during subculture. Approximately 10% of the total cell population continued to express markers for Stro1+/ALP− cells throughout.

Conclusions

These data suggest periosteum contains a large number of undifferentiated cells that can differentiate into neotissue and persist despite culture in noncell-specific media for over 10 passages.

Clinical Relevance

Cultured periosteal cells may contribute to tissue formation and may be applicable for tissue engineering applications.

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Acknowledgments

We thank Natasa Devic, MB BS, for experimental and analytical support on many aspects of this study. In addition, we thank Maria Azevedo and Steve Mwenifumbo for their help counting and scoring CFU dishes.

Author information

Authors and Affiliations

  1. Department of Materials, Royal School of Mines, Imperial College London, London, UK

    Michael D. Ball PhD, Ian C. Bonzani PhD & Melissa J. Bovis BSc

  2. Institute for Biomedical Engineering, Imperial College London, London, UK

    Ian C. Bonzani PhD

  3. Chelsea and Westminster Healthcare NHS Trust, Chelsea, London, UK

    Andrew Williams MBBS

  4. Department of Materials and Institute for Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK

    Molly M. Stevens PhD

Authors
  1. Michael D. Ball PhD
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  2. Ian C. Bonzani PhD
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Corresponding author

Correspondence to Molly M. Stevens PhD.

Additional information

One or more of the authors (MMS) received funding from EPSRC (grant number EP/C520742/1) and the Leverhulme Trust. One of the authors (ICB) received funding from the Marshall Aid Commemoration Commission. One of the authors (AW) received funding from the British Association of the Knee.

Each author certifies that his or her institution has approved the reporting of these cases, that all investigations were conducted in conformity with ethical principles of research, and that informed consent for participating in the study was obtained.

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Ball, M.D., Bonzani, I.C., Bovis, M.J. et al. Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study. Clin Orthop Relat Res 469, 3085–3093 (2011). https://doi.org/10.1007/s11999-011-1895-x

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  • DOI: https://doi.org/10.1007/s11999-011-1895-x

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