Skip to main content
Log in

Effect of photoperiod and light intensity on in vitro propagation of Alocasia amazonica

  • Original Article
  • Published:
Plant Biotechnology Reports Aims and scope Submit manuscript

Abstract

Plantlets of Alocasia amazonica regenerated under a photon flux density (PFD) of 15 or 30 μmol m−2 s−1 showed better growth and development than those grown under higher PFDs. While chlorophyll a and chlorophyll b decreased, the number of stomata increased with increasing PFD. Photoperiods also affected plantlet growth and stomatal development. Highest growth was observed for the short photoperiod (8/16 h) and for equinoctial (12/12 h) light and dark periods. Very few stomata developed in the leaves of plantlets grown under a short photoperiod (8/16 h) and the number of stomata increased with increasing light period. In conclusion, both light intensity and photoperiod independently affect growth of A. amazonica and development of stomata, depending on the intensity and duration of light treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Ali MB, Hahn EJ, Paek KY (2005) Effect of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalaenopsis plantlet. Environ Exp Bot 54:109–120

    Article  Google Scholar 

  • Amâncio S, Rebordão JP, Chaves MM (1999) Improvement of acclimatization of micropropagated grapevine: Photosynthetic competence and carbon allocation. Plant Cell Tiss Org Cult 58:31–37

    Article  Google Scholar 

  • Anderson JM, Chow WS, Park Y-I (1995) The grand design of photosynthesis: acclimation of the photosynthetic apparatus to environmental cues. Photosynth Res 46:129–139

    Article  Google Scholar 

  • Anderson JM, Osmond CB (1987) Shade-sun responses: compromises between acclimation and photoinhibition. In: Kyle DJ, Osmond CB, Arntzen CJ (eds) photoinhibition. Elsevier, Amsterdam, pp 1–38

    Google Scholar 

  • Björkman O (1973) Comparative studies on photosynthesis in higher plants. In: Giese A (ed) Current topics in photobiology, photochemistry and photophysiology, vol 8. Academic Press, NY, pp 1–63

    Google Scholar 

  • Demmig-Adams B, Adams III (1992) Photoprotection and other responses of plants to high light stress. Annu Rev Plant Physiol Plant Mol Biol 43:599–626

    Article  CAS  Google Scholar 

  • Dimassi-Theriou K, Bosabalidis AM (1997) Effects of light, magnesium and sucrose on leaf anatomy, photosynthesis, starch and total sugar accumulation, in kiwifruit cultured in vitro. Plant Cell Tiss Org Cult 47:127–134

    Article  Google Scholar 

  • Gray JD, Kolesik P, Hoj PB, Coombe BG (1999) Confocal measurement of the three-dimensional size and shape of plant parenchyma cells in a developing fruit tissue. Plant J 19:229–236

    Article  PubMed  Google Scholar 

  • Heo JW, Lee CW, Murthy HN, Paek KY (2003) Influence of light quality and photoperiod on flowering of Cyclamen persicum Mill. cv. Dixie White. Plant Growth Reg 40:7–10

    Article  Google Scholar 

  • Jeon MW, Ali MB, Hahn EJ, Paek KY (2005) Effect of photon flux density on the morphology, photosynthesis, and growth of a CAM orchid, Doritaenopsis during post-micropropagation acclimatization. Plant Growth Reg 45:139–147

    Article  Google Scholar 

  • Khokhar KM, Hadley P, Pearson S (2007) Effect of photoperiod and temperature on inflorescence appearance and subsequent development towards flowering in onion raised from sets. Sci Hotic 112:9–15

    Article  Google Scholar 

  • Lee SH, Tewari RK, Hahn EJ, Paek KY (2007) Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania somnifera (L.) Dunal. plantlets. Plant Cell Tiss Org Cult 90:141–151

    Article  CAS  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148:350–382

    Article  CAS  Google Scholar 

  • Morini S, Trinci M, Zacchini M (1991) Effect of different photoperiods on in vitro growth of Mr.S.2/5 plum rootstock. Plant Cell Tiss Org Cult 25:141–145

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murchie EH, Horton P (1997) Acclimation of photosynthesis to irradiance and spectral quality in British plant species: chlorophyll content, photosynthetic capacity and habitat preference. Plant Cell Environ 20:438–448

    Article  Google Scholar 

  • Omokolo ND, Boudjeko T, Tsafack Takadong JJ (2003) In vitro tuberization of Xanthosoma sagittifolium L. Schott: Effects of phytohormones, sucrose, nitrogen and photoperiod. Sci Hortic 98:337–345

    Article  Google Scholar 

  • Osmond CB (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In: Baker NR, Bowter JR (eds) Photoinhibition of photosynthesis: from molecular mechanisms to the field. BIOS Scientific Oxford, pp 1–24

  • Paek KY (2001) Regulations of microenvironments. In: Paek KY (ed) Plant tissue culture. Hyangmunsa, Seoul, Korea, pp 341–361

    Google Scholar 

  • Pandey DM, Kang K-H, Yeo U-D (2005) Effects of excessive photon on the photosynthetic pigments and violaxanthin de-epoxidase activity in the xanthophylls cycle of spinach leaf. Plant Sci 168:161–166

    Article  Google Scholar 

  • Powles SB (1984) Photoinhibition of photosynthesis induced by visible light. Annu Rev Plant Physiol 35:15–44

    Article  CAS  Google Scholar 

  • Ramanayake SMSD, Meemaduma VN, Weerawardene TE (2006) In vitro shoot proliferation and enhancement of rooting for the large-scale propagation of yellow bamboo (Bambusa vulgaris ‘Striata’). Sci Hortic 110:109–113

    Article  Google Scholar 

  • Stadler R, Büttner M, Ache P, Hedrich R, Ivashikina N, Melzer M, Shearson SM, Smith SM, Sauer N (2003) Diurnal and light-regulated expression of AtSTP1 in guard cells of Arabidopsis. Plant Physiol 133:528–537

    Article  PubMed  Google Scholar 

  • Stewart SL, Kane ME (2006) Symbiotic seed germination of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tiss Org Cult 86:159–167

    Article  Google Scholar 

  • Taiz L, Zeiger E (2002) Plant Physiology, 3rd edn. Sinauer Associates, Inc., Sunderland, MA

    Google Scholar 

  • Walters RG, Shephard F, Rogers JJM, Rolfe SA, Horton P (2003) Identification of mutants of Arabidopsis defective in acclimation of photosynthesis to the light environment. Plant Physiol 131:472–481

    Article  PubMed  Google Scholar 

  • Weatherley PE (1950) Studies in the water relations of the cotton plant. 1. the field measurement of water deficit in leaves. New Phytol 49:81–97

    Article  Google Scholar 

Download references

Acknowledgments

This work is financially supported by the Ministry of Education and Human Resource Development (MOE), the Ministry of Commerce, Industry and Energy (MOCIE) and Ministry of Labor (MOLAB) through the fostering project of Laboratories of Excellence.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kee-Yoeup Paek.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jo, EA., Tewari, R.K., Hahn, EJ. et al. Effect of photoperiod and light intensity on in vitro propagation of Alocasia amazonica . Plant Biotechnol Rep 2, 207–212 (2008). https://doi.org/10.1007/s11816-008-0063-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11816-008-0063-6

Keywords

Navigation