Abstract
In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Lycopersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO (0.05%, 0.10%, 0.20%, 0.30% and 0.50%) after drought stress was simulated by exposing the roots to 0.6 molL−1 PEG-6000 solution for 6 h. Changes in biomass, electrolyte leakage and malondialdehyde (MDA), free proline, total soluble sugars (TSS) and abscisic acid (ABA), the enzyme activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) were measured to investigate the effects of ADO treatment. The results showed that the treatment with an ADO concentration of 0.20% exhibited the highest performance of drought stress resistance in the tomato seedlings by decreasing the electrolyte leakage and the concentration of MDA, increasing the contents of free proline, TSS and ABA, and increasing the activities of CAT, SOD, POD and PAL after treatment with ADO. It is suggested that changes in electrolyte leakage, MDA, osmotic solutes, ABA, anti-oxidative enzyme and PAL activities were responsible for the increased drought stress resistance in tomato seedlings. To our best knowledge, this is the first report of the effect of ADO treatment on enhancing the drought stress resistance of tomato seedlings.
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References
Albersheim, P., and Darvill, A. G., 1985. Oligosaccharins: novel molecules that can regulate growth, development, reproduction, and defense against disease in plants. Sci. Am., 253: 58–64.
Bates, L. S., Waldren, R. P., and Teare, I. D., 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205–207.
Bray, E. A., 2002. Abscisic acid regulation of gene expression during water-deficit stress in the era of the Arabidopsis genome. Plant Cell Environ., 25: 153–161.
Campalans, A., Messeguer, R., Goday, A., and Pages, M., 1999. Plant responses to drought, from ABA signal transduction events to the action of the induced proteins. Plant Physiol. Biochem., 37: 327–340.
Chandler, P. M., and Robertson, M., 1994. Gene expression regulated by abscisic acid and its relation to stress tolerance. Annu., Rev. Plant Physiol. Plant Mol. Biol., 45: 113–141.
Dixon, R. D., and Paiva N. L., 1995. Stress-induced phenylpropanoid metabolism. Plant Cell, 7: 1085–1097.
Foolad, M. R., 2007. Current status of breeding tomatoes for salt and drought tolerance. In: Advances in Molecular Breeding Toward Drought and Salt Tolerant Crops. Jenks, M. A., et al., eds., Springer, Netherlands, 669–700.
Giannopolitis, C. N., and Ries, S. K., 1977. Superoxide dismutase occurrence in higher plants. Plant Physiol., 59: 309–314.
Glombitza, C., Dubuis, P. H., Thulke, O., Glombitza, C., Dubuis, P. H., and Thulke, O., 2004. Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism. Plant Mol. Biol., 51: 1–19.
Hara, M., Terashima, S., Fukaya, T. and Kubom T., 2003. Enhancement of cold tolerance and inhibition of lipid peroxidation by citrus dehydrin in transgenic tobacco. Planta, 217: 290–298.
Hartung, W., Schraut, D., and Jiang, F., 2005. Physiology of abscisic acid (ABA) in roots under stress-a review of the relationship between root ABA and radial water and ABA flows. Aust. J. Agric. Res., 56: 1253–1259.
Hien, N. Q., Nagasawa, N., Tham, L. X., Yoshii, F., Dang, V. H., Mitomo, H., et al., 2000. Growth-promotion of plants with depolymerized alginate by irradiation. Radiat. Phys. Chem., 59: 97–101.
Hu, X. K., Jiang, X. L., Hwang, H. M., Liu, S. L., and Guan, H. S., 2004. Promotive effects of alginate-derived oligosaccharide on maize seed germination. J. Appl. Phycol., 16: 73–76.
Iwasaki, K. I., and Matsubara, Y., 2000. Purification of pectate oligosaccharides showing root-growth-promoting activity in lettuce using ultrafiltration and nanofiltrat. J. Biosci. Bioeng., 89: 495–497.
Jitratham, A., Yazama, F., and Kondo, S., 2006. Effects of drought stress on abscisic acid and jasmonate metabolism in citrus. Environ. Cont. Biol., 44: 41–49.
Kondo, S., Kittikorn, M., Ohara, H., Okawa, K., Sugaya, S., Kitahata, N., et al., 2009. Effect of a cytochrome P450 inhibitor on abscisic acid biosynthesis and stomatal regulation in citrus trees in water-stressed conditions. Sci. Hortic., 120: 146–149.
Larson, R. A., 1988. The antioxidants of higher plants. Phytochemistry, 27: 969–978.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265–275.
Menconi, M., Sgherri, C. L. M., Pinzino, C., and Navari-Izzo, F., 1995. Activated oxygen production and detoxication in wheat plants subjected to a water deficit programme. J. Exp. Bot., 46(290): 1123–1130.
Natsume, M., Kamo, Y., Hirayama, M., and Adachi, T., 1994. Isolation and characterization of alginate derived oligosaccharides with root growth promoting activities. Carbohydr. Res., 258: 187–197.
Patnaitk, D., and Khurana, P., 2001. Wheat biotechnology: a mini-review. Electron. J. Biotechnol., 4: 74–102.
Peltonen, S., and Karjalainen, R., 1995. Phenylalanine ammonialyase activity in barley after infection with Bipolaris sorokiniana or treatment with purified xylanase. J. Phytopathol., 143: 239–245.
Qiu, Z. B., Liu, X., Tian, X. J., and Yue, M., 2008. Effect of CO2 laster pretreatment on drought stress resistance in wheat. J. Photochem. photobiol., B: Biology, 90: 17–25.
Riaze, A., Matsuda, K., and Arslan, A., 1985. Water-stress induced changes in concentrations of praline and other solute in growing regions of young barley leaves. J. Exp. Bot., 36: 1716–1725.
Smirnoff, N., 1993. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol., 125: 27–58.
Solecka, D., 1997. Role of phenylpropanoid compounds in plant responses to different stress factors. Acta Physiol. Plant, 19: 257–268.
Solecka, D., and Kacperska, A., 2003. Phenylpropanoid deficiency affects the course of plant acclimation to cold. Physiol. Plant, 119: 253–262.
Tanaka, K., Masuda, R., Sugimoto, T., Omasa, K., and Sakaki, Z., 1990. Water deficiency-induced changes in the contents of defensive substances against active oxygen in spinach leaves. Agric. Biol. Chem., 54: 2629–2634.
Thomashow, M. F., 1999. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu. Rev. Plant Physiol. Plant Mol. Biol., 50: 571–599.
Verslues, P. E., and Bray, E. A., 2006. Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation. J. Exp. Bot., 57: 201–212.
Walker-Simmons, M. K., and Abrams, S. R., 1991. Use of ABA immunoassays, In: Abscisic Acid Physiology and Biochemistry. Davies, W. J., and Jones, H. G., eds., Bios Scientific Publishers, Oxford, 53–63.
Wang, W. X., Vinocur, B., and Altman, A., 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218(1): 1–14.
Zhang, C. X., 2007. Study on the export of tomato products in China. Master thesis. Huazhong Agriculture University, Wuhan, 1–50.
Zhang, J. X., and Kirham, M. B., 1994. Drought stress-induced changes in activities of superoxide dismutase, catalase and peroxidase in wheat species. Plant Cell Physiol., 35(5): 785–791.
Zhang, J., Jia, W., Yang, J., and Ismail, A. M., 2006. Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Res., 97: 111–119.
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Liu, R., Jiang, X., Guan, H. et al. Promotive effects of alginate-derived oligosaccharides on the inducing drought resistance of tomato. J. Ocean Univ. China 8, 303–311 (2009). https://doi.org/10.1007/s11802-009-0303-6
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DOI: https://doi.org/10.1007/s11802-009-0303-6