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Medium-Chain Enriched Diacylglycerol (MCE-DAG) Oil Decreases Body Fat Mass in Mice by Increasing Lipolysis and Thermogenesis in Adipose Tissue

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Lipids

Abstract

Medium chain fatty acid (MCFA) escapes the formation of chylomicrons in the small intestine, resulting in energy expenditure through beta-oxidation. Diacylglycerol (DAG) is susceptible to oxidation rather than being stored in the adipose tissue. This study was conducted to verify the effect of MCE-DAG oil on body fat mass in vivo. Male C57BL/6 mice were randomly assigned to four groups (n = 12) as follows: (1) normal diet (18% kcal from fat), (2) canola oil as a control (40% kcal from canola oil), (3) MCE-DAG10 (10% kcal from MCE-DAG + 30% kcal from canola oil), and (4) MCE-DAG20 (20% kcal from MCE-DAG + 20% kcal from canola oil). The body weight and fat mass of MCE-DAG20 group mice were decreased relative to those of control mice (P < 0.05 and P < 0.001, respectively). Serum triacylglycerol (TAG) was decreased in both MCE-DAG10 and MCE-DAG20 groups (P < 0.01 and P < 0.05, respectively). Hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) were increased in the MCE-DAG20 group relative to the control in white adipose tissue (WAT) (P < 0.05). Uncoupling protein 1 (UCP1) was also increased in the MCE-DAG20 group relative to the control in brown adipose tissue (BAT) (P < 0.05). In summary, MCE-DAG reduced body fat mass likely by stimulating lipolysis in WAT and thermogenesis in BAT.

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Abbreviations

ACC:

Acetyl CoA carboxylase

ATGL:

Adipose triglyceride lipase

BAT:

Brown adipose tissue

DAG:

Diacylglycerol

DEXA:

Dual-energy X-ray absorptiometry

FAS:

Fatty acid synthase

GAPDH:

Glyceraldehyde-3-phophate dehydrogenase

HSL:

Hormone sensitive lipase

LC-TAG:

Long-chain triacylglycerol

LDL-C:

Low-density lipoprotein cholesterol

MAG:

Monoacylglycerol

MCE-DAG:

Medium-chain enriched diacylglycerol

MCFA:

Medium-chain fatty acid

MC-TAG:

Medium-chain triacylglycerol

MLC-TAG:

Medium- and long-chain triacylglycerol

MUFA:

Monounsaturated fatty acid

PUFA:

Polyunsaturated fatty acid

SFA:

Saturated fatty acid

SREBP1:

Sterol regulatory element binding protein 1

TAG:

Triacylglycerol

TC:

Total cholesterol

UCP1:

Uncoupling protein 1

WAT:

White adipose tissue

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Authors and Affiliations

  1. Department of Foods and Nutrition, Kookmin University, 77 Jeongneung-ro, Seongbuk-gu, Seoul, 02707, Korea

    Haeun Kim, Jee-Hwan Choe & Gwang-woong Go

  2. R&D Center, Nongshim, Seoul, Korea

    Jong Hun Choi, Hun Jung Kim & Soo Hyun Park

  3. Department of Public Health Science, Korea University, Seoul, Korea

    Moon Won Lee

  4. Department of Food Science and Biotechnology, Kyung Hee University, Yongin, Korea

    Wooki Kim

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  1. Haeun Kim
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Correspondence to Gwang-woong Go.

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Kim, H., Choe, JH., Choi, J.H. et al. Medium-Chain Enriched Diacylglycerol (MCE-DAG) Oil Decreases Body Fat Mass in Mice by Increasing Lipolysis and Thermogenesis in Adipose Tissue. Lipids 52, 665–673 (2017). https://doi.org/10.1007/s11745-017-4277-7

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