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Fatty Acids Have Different Adipogenic Differentiation Potentials in Stromal Vascular Cells Isolated from Abdominal Fat in Laying Hens

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Lipids

Abstract

This study was conducted to examine the effects of fatty acids (FA) with/without chicken serum (CS) on the expression of adipogenic transcripts and adipogenesis in chicken stromal vascular cells (SVC). In experiment 1, SVC were grown in DMEM containing 10% FBS (Control) and treated with 300 µM oleic acid (OLA) + FBS, linoleic acid (LNA) + FBS, palmitic acid (PAM) + FBS, or stearic acid (STA) + FBS for 48 h. In experiment 2, cells were grown in DMEM containing 5% CS and treated with 300 µM OLA (CS + OLA), PAM (CS + PAM), STA (CS + STA) or 200 µM LNA (CS + LNA) for 48 h. Adipogenesis was determined using Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity. The proportion of OLA, PAM, or STA was increased (P < 0.05) in SVC grown in either FBS or CS with OLA, PAM or STA. Adipogenesis was induced in FBS + OLA, FBS + LNA, FBS + PAM, FBS + STA, CS + OLA, CS + LNA, CS + PAM, or CS + SAT compared to FBS. GPDH activity was significantly higher in FBS + OLA and FBS + LNA than one in FBS. Compared to FBS, the expression of FABP4 mRNA increased (P < 0.05) in FBS + OLA, FBS + LNA, or FBS + PAM, whereas that of C/EBPα, C/EBPβ, and ATGL increased (P < 0.05) in FBS + OLA or FBS + LNA cells. Expression of FABP4 and C/EBPβ mRNA was higher in CS, CS + OLA, CS + LNA, CS + PAM, or CS + SAT compared with (FBS, whereas the expression of ATGL and C/EBPα was higher in CS, CS + OLA, or CS + LNA than FBS cells. In conclusion, these results showed that FA have different potentials to induce adipogenesis, LNA is the most potent among the tested FA, and these potentials can be improved in the presence of CS.

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Abbreviations

CS:

Chicken serum

OLA:

Oleic acid

LNA:

Linoleic acid

PAM:

Palmitic acid

STA:

Stearic acid

hr:

Hour

CLA:

Conjugated linoleic acid

MSC:

Mesenchymal stem cell

FABP4:

Fatty acid binding protein 4

PPARγ2:

PPAR gamma2

C/EBPα:

CCAAT/enhancer binding protein alpha

C/EBPβ:

CCAAT/enhancer binding protein beta

ATGL:

Adipocyte triglyceride lipase

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

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Authors and Affiliations

  1. Department of Animal Science, University of Manitoba, Winnipeg, MB, Canada

    Alemu Regassa

  2. Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Canada

    Miyoung Suh & Jutika Datar

  3. Department of Poultry Science, University of Georgia, 303 Poultry Science Building, Athens, GA, 30602, USA

    Chongxiao Chen & Woo Kyun Kim

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  1. Alemu Regassa
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  2. Miyoung Suh
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  3. Jutika Datar
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  4. Chongxiao Chen
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  5. Woo Kyun Kim
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Correspondence to Woo Kyun Kim.

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Regassa, A., Suh, M., Datar, J. et al. Fatty Acids Have Different Adipogenic Differentiation Potentials in Stromal Vascular Cells Isolated from Abdominal Fat in Laying Hens. Lipids 52, 513–522 (2017). https://doi.org/10.1007/s11745-017-4261-2

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  • DOI: https://doi.org/10.1007/s11745-017-4261-2

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