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Non-conjugated cis/trans 18:2 in Beef Fat are Mainly Δ-9 Desaturation Products of trans-18:1 Isomers

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Lipids

Abstract

Human liver cells (HepG2) were cultured with individual trans (t) 18:1 including t6-, t12-, t13-, t14-, t15- and t16-18:1, and retention times of their Δ-9 desaturation products were determined using 100-m biscyanopropyl-polysiloxane and SLB-IL111 columns. Corresponding peaks were found in beef adipose tissues known to have different delta-9 desaturase activities. Further lines of evidence indicating the presence of Δ-9 desaturation products of t-18:1 isomers in beef fat were developed by analysis of fatty acid methyl esters (FAME) fractionated using Ag+-TLC, and by GC/MS. Some of the Δ-9 desaturation products of t-18:1 have been previously identified in ruminant fat (c9, t12- and c9, t13-18:2). Some of the Δ-9 desaturation products of t-18:1 (c9, t14- and c9, t15-18:2) have been previously tentatively identified as different fatty acids, and for the first time we provide evidence of the presence of c9, t16-18:2, and where t6, c9-18:2 may elute during analysis of FAME from beef fat.

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Abbreviations

Ag+-HPLC:

Silver-ion high performance liquid chromatography

Ag+-SPE:

Silver-ion solid phase extraction

BSA:

Bovine serum albumin

c :

cis

cyclo-170:

Cyclohexylundecanoic acid

FAME:

Fatty acid methyl ester(s)

FBS:

Fetal bovine serum

MUFA:

Monounsaturated fatty acid(s)

PRF:

Kidney perirenal fat

SCF:

Beef subcutaneous fat

t :

trans

TAG:

Triacylglycerol

TLC:

Thin layer chromatography

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Acknowledgements

Study funding was provided through AAFC-Peer Review program (Grant#13-1144). P. Vahmani acknowledges NSERC post-doctoral funding provided by the AAFC-Peer Review program.

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Authors and Affiliations

  1. Lacombe Research and Development Centre, Agriculture and Agri-Food Canada, Lacombe, AB, T4L 1W1, Canada

    P. Vahmani, D. C. Rolland, K. E. Gzyl & M. E. R. Dugan

Authors
  1. P. Vahmani
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  2. D. C. Rolland
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  3. K. E. Gzyl
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  4. M. E. R. Dugan
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Corresponding author

Correspondence to M. E. R. Dugan.

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Vahmani, P., Rolland, D.C., Gzyl, K.E. et al. Non-conjugated cis/trans 18:2 in Beef Fat are Mainly Δ-9 Desaturation Products of trans-18:1 Isomers. Lipids 51, 1427–1433 (2016). https://doi.org/10.1007/s11745-016-4207-0

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  • DOI: https://doi.org/10.1007/s11745-016-4207-0

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