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Bis(monoacylglycero)phosphate as a Macrophage Enriched Phospholipid

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Lipids

Abstract

Bis(monoacylglycero)phosphate (BMP) is a structural isomer of phosphatidylglycerol (PtdGro) with an unusual sn-1:sn-1′ fatty acyl configuration and is found almost exclusively in late endosomes/lysosomes. BMP comprises only about 1–2 % of the total phospholipids in most mammalian cells, but accumulates in tissues of humans and animals with lysosomal storage disorders including the gangliosidoses. Total BMP content was significantly greater in cells of macrophage/microglial origin than in cells of macroglial origin. BMP composition was similar in tumorigenic/metastatic macrophages and non-tumorigenic macrophages/microglia. Finally, BMP fatty acid composition differed between cells grown in culture and obtained in vivo suggesting an influence from growth environment.

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Fig. 1

Abbreviations

BMP:

Bis(monoacylglycero)phosphate

Ptd2Gro:

Cardiolipin

PtdOH:

Phosphatidic acid

PtdGro:

Phosphatidylglycerol

PtdSer:

Phosphatidylserine

PtsIns:

Phosphatidylinositol

HPTLC:

High-performance thin-layer chromatography

References

  1. Brotherus J, Renkonen O (1977) Subcellular distributions of lipids in cultured BHK cells: evidence for the enrichment of lysobisphosphatidic acid and neutral lipids in lysosomes. J Lipid Res 18(2):191–202

    CAS  PubMed  Google Scholar 

  2. Kobayashi T et al (2001) Localization of lysobisphosphatidic acid-rich membrane domains in late endosomes. Biol Chem 382(3):483–485

    Article  CAS  PubMed  Google Scholar 

  3. Brotherus J et al (1974) Novel stereoconfiguration in lyso-bis-phosphatidic acid of cultured BHK-cells. Chem Phys Lipids 13(2):178–182

    Article  CAS  PubMed  Google Scholar 

  4. Tan HH et al (2012) Spectroscopic evidence for the unusual stereochemical configuration of an endosome-specific lipid. Angew Chem 51(2):533–535

    Article  CAS  Google Scholar 

  5. Hullin-Matsuda F et al (2009) Bis(monoacylglycero)phosphate, a peculiar phospholipid to control the fate of cholesterol: Implications in pathology. Prostaglandins Leukot Essent Fatty Acid 81(5–6):313–324

    Article  CAS  Google Scholar 

  6. Mason RJ, Stossel TP, Vaughan M (1972) Lipids of alveolar macrophages, polymorphonuclear leukocytes, and their phagocytic vesicles. J Clin Investig 51(9):2399–2407

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Wherrett JR, Huterer S (1973) Bis-(monoacylglyceryl)-phosphate of rat and human liver: fatty acid composition and NMR spectroscopy. Lipids 8(9):531–533

    Article  CAS  PubMed  Google Scholar 

  8. Luquain C et al (2000) Bis (monoacylglycerol) phosphate in rat uterine stromal cells: structural characterization and specific esterification of docosahexaenoic acid. Biochem J 351(Pt 3):795–804

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Besson N et al (2006) Selective incorporation of docosahexaenoic acid into lysobisphosphatidic acid in cultured THP-1 macrophages. Lipids 41(2):189–196

    Article  CAS  PubMed  Google Scholar 

  10. Bouvier J et al (2009) Selective decrease of bis(monoacylglycero)phosphate content in macrophages by high supplementation with docosahexaenoic acid. J Lipid Res 50(2):243–255

    Article  CAS  PubMed  Google Scholar 

  11. Matsuo H et al (2004) Role of LBPA and Alix in multivesicular liposome formation and endosome organization. Science 303(5657):531–534

    Article  CAS  PubMed  Google Scholar 

  12. Chevallier J et al (2008) Lysobisphosphatidic acid controls endosomal cholesterol levels. J Biol Chem 283(41):27871–27880

    Article  CAS  PubMed  Google Scholar 

  13. Gallala HD, Sandhoff K (2011) Biological function of the cellular lipid BMP-BMP as a key activator for cholesterol sorting and membrane digestion. Neurochem Res 36(9):1594–1600

    Article  CAS  PubMed  Google Scholar 

  14. Sandhoff K (2013) Metabolic and cellular bases of sphingolipidoses. Biochem Soc Trans 41(6):1562–1568

    Article  CAS  PubMed  Google Scholar 

  15. Sandhoff K, Harzer K (2013) Gangliosides and gangliosidoses: principles of molecular and metabolic pathogenesis. J neurosci: off j Soc for Neurosci 33(25):10195–10208

    Article  CAS  Google Scholar 

  16. Hein LK, Duplock S, Fuller M (2013) Selective reduction of bis(monoacylglycero)phosphate ameliorates the storage burden in a THP-1 macrophage model of Gaucher disease. J Lipid Res 54(6):1691–1697

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Meikle PJ et al (2008) Effect of lysosomal storage on bis(monoacylglycero)phosphate. Biochem J 411(1):71–78

    Article  CAS  PubMed  Google Scholar 

  18. Rouser G et al (1968) Accumulation of a glycerolphospholipid in classical niemann-pick disease. Lipids 3(3):287–290

    Article  CAS  PubMed  Google Scholar 

  19. Akgoc Z et al (2015) Bis(monoacylglycero)phosphate: a secondary storage lipid in the gangliosidoses. J Lipid Res 56(5):1006–1013

    Article  CAS  PubMed  Google Scholar 

  20. Renate L.R., Lysosomes. 2005. Medical Intelligence Unit. Springer:US

  21. Huysentruyt LC et al (2008) Metastatic cancer cells with macrophage properties: evidence from a new murine tumor model. Int J Cancer 123(1):73–84

    Article  CAS  PubMed  Google Scholar 

  22. Seyfried TN (1992) M. el-Abbadi, and M.L. Roy, Ganglioside distribution in murine neural tumors. Mol Chem Neuropathol 17(2):147–167

    Article  CAS  PubMed  Google Scholar 

  23. Seyfried NT et al (2008) Up-regulation of NG2 proteoglycan and interferon-induced transmembrane proteins 1 and 3 in mouse astrocytoma: A membrane proteomics approach. Cancer Lett 263(2):243–252

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Huang FJ et al (2010) Pericyte deficiencies lead to aberrant tumor vascularizaton in the brain of the NG2 null mouse. Dev Biol 344(2):1035–1046

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  25. Binello E et al (2012) Stemness of the ct-2a immunocompetent mouse brain tumor model: characterization in vitro. J Cancer 3:166–174

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Yohe HC, Coleman DL, Ryan JL (1985) Ganglioside alterations in stimulated murine macrophages. Biochim Biophys Acta 818(1):81–86

    Article  CAS  PubMed  Google Scholar 

  27. Ecsedy JA et al (1998) Tumor-infiltrating macrophages influence the glycosphingolipid composition of murine brain tumors. J Lipid Res 39(11):2218–2227

    CAS  PubMed  Google Scholar 

  28. Baek RC et al (2008) N-butyldeoxygalactonojirimycin reduces brain ganglioside and GM2 content in neonatal Sandhoff disease mice. Neurochem Int 52(6):1125–1133

    Article  CAS  PubMed  Google Scholar 

  29. Seyfried TN, Glaser GH, Yu RK (1978) Cerebral, cerebellar, and brain stem gangliosides in mice susceptible to audiogenic seizures. J Neurochem 31(1):21–27

    Article  CAS  PubMed  Google Scholar 

  30. Macala LJ, Yu RK, Ando S (1983) Analysis of brain lipids by high performance thin-layer chromatography and densitometry. J Lipid Res 24(9):1243–1250

    CAS  PubMed  Google Scholar 

  31. Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509

    CAS  PubMed  Google Scholar 

  32. Ta NL et al (2014) Autosomal dominant inheritance of brain cardiolipin fatty acid abnormality in vm/dk mice: association with hypoxic-induced cognitive insensitivity. Lipids 49(1):113–117

    Article  CAS  PubMed  Google Scholar 

  33. Orso E, Grandl M, Schmitz G (2011) Oxidized LDL-induced endolysosomal phospholipidosis and enzymatically modified LDL-induced foam cell formation determine specific lipid species modulation in human macrophages. Chem Phys Lipids 164(6):479–487

    Article  CAS  PubMed  Google Scholar 

  34. Chapuy-Regaud S et al (2013) Progesterone and a phospholipase inhibitor increase the endosomal bis(monoacylglycero)phosphate content and block HIV viral particle intercellular transmission. Biochimie 95(9):1677–1688

    Article  CAS  PubMed  Google Scholar 

  35. Roth SL, Whittaker GR (2011) Promotion of vesicular stomatitis virus fusion by the endosome-specific phospholipid bis(monoacylglycero)phosphate (BMP). FEBS Lett 585(6):865–869

    Article  CAS  PubMed  Google Scholar 

  36. Liu T et al (2011) Calcium triggers exocytosis from two types of organelles in a single astrocyte. J Neurosci 31(29):10593–10601

    Article  CAS  PubMed  Google Scholar 

  37. Li D et al (2008) Lysosomes are the major vesicular compartment undergoing Ca2 + -regulated exocytosis from cortical astrocytes. J Neurosci 28(30):7648–7658

    Article  CAS  PubMed  Google Scholar 

  38. Kiebish MA et al (2007) Mitochondrial lipid and electron transport chain abnormalities in mouse brain tumors. J Neurochem 102(Suppl. 1):64

    Google Scholar 

  39. Raschke WC et al (1978) Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15(1):261–267

    Article  CAS  PubMed  Google Scholar 

  40. Shelton LM et al (2010) A novel pre-clinical in vivo mouse model for malignant brain tumor growth and invasion. J Neurooncol 99(2):165–176

    Article  CAS  PubMed  Google Scholar 

  41. Seyfried T.N., L.M. Shelton, and L.C. Huysentruyt 2012. The VM mouse model of glioblastoma multiforme, in neuromethods, R.a.M. Martinez—Murillo A., Editor. Springer Science + Business Media, LLC

  42. Huysentruyt LC, Seyfried TN (2010) Perspectives on the mesenchymal origin of metastatic cancer. Cancer Metastasis Rev 29(4):695–707

    Article  PubMed Central  PubMed  Google Scholar 

  43. Huysentruyt L.C., D. Banerjee and T.N. Seyfried 2007. Cancer metastasis as a macrophage disease: Evidence from a new mouse tumor model. American Association for Cancer Research Annual Meeting: Proceedings, 2007(5487)

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Acknowledgments

This work was supported in part by National Institutes of Health Grant NS-055195 and Boston College Research Expense Fund.

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Authors and Affiliations

  1. Biology Department, Boston College, 140 Commonwealth Ave, MA, 02467, Chestnut Hill, USA

    Zeynep Akgoc, Sonia Iosim & Thomas N. Seyfried

Authors
  1. Zeynep Akgoc
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  2. Sonia Iosim
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  3. Thomas N. Seyfried
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Corresponding author

Correspondence to Thomas N. Seyfried.

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Akgoc, Z., Iosim, S. & Seyfried, T.N. Bis(monoacylglycero)phosphate as a Macrophage Enriched Phospholipid. Lipids 50, 907–912 (2015). https://doi.org/10.1007/s11745-015-4045-5

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  • DOI: https://doi.org/10.1007/s11745-015-4045-5

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