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Exogenous ascorbic acid mitigates accumulation of abscisic acid, proline and polyamine under osmotic stress in maize leaves

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Abstract

The effects of osmotic stress (polyethylene glycol, PEG 6000) and exogenous ascorbic acid (ASC) on abscisic acid (ABA), some osmolytes (proline and polyamine), and gene expression of polyamine metabolic enzymes arginine decarboxylase (ADC), S-adenosylmethionine decarboxylase (SAMDC) and polyamine oxidase (PAO1) were assessed in the leaves of Zea mays L. seedlings. ASC (0.1 mM) was hydroponically applied to detached maize seedlings. ASC application scavenged endogenous hydrogen peroxide level and ameliorated leaf water status and stomatal conductance under osmotic stress. Lipid peroxidation, ABA and osmolyte accumulation were mitigated by ASC treatment. For osmolytes, the alleviation of polyamine accumulation was more obvious than that of the proline. Therefore, gene expressions of polyamine metabolic enzymes were investigated. Compared to control seedlings, down-regulation of SAMDC and ADC gene expressions were determined in ASC + PEG-applied seedlings while PAO1 gene expression increased. These results indicated that exogenous ASC influenced polyamine metabolism. ASC application under the osmotic stress ameliorated osmotic solute levels and ABA concentration in maize.

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Abbreviations

ABA:

Abscisic acid

ABRE:

Abscisic acid-responsive element

ADC:

Arginine decarboxylase

ASC:

Ascorbic acid

DW:

Dry weight

g s :

Stomatal conductance

HPLC:

High performance liquid chromatography

MDA:

Malondialdehyde

PA:

Polyamine

PAO:

Polyamine oxidase

PEG:

Polyethylene glycol

Put:

Putrescine

ROS:

Reactive oxygen species

SAMDC:

S-Adenosylmethionine decarboxylase

Spd:

Spermidine

SPDS:

Spermidine synthase

Spm:

Spermine

SPMS:

Spermine synthase

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Acknowledgments

This work is supported by Turkish National Science Foundation (Project No: 111T511). We thank Assoc. Prof. Dr. Ahmet YAŞAR (Faculty of Pharmacy, Karadeniz Technical University) for their assistance with analysis of HPLC. We also wish to thank lecturer Ali Şükrü ÖZBAY (School of Foreign Languages, Karadeniz Technical University) for English language improvement.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Rabiye Terzi, Ebru Kalaycıoglu, Mehmet Demiralay & Asim Kadioglu

  2. Department of Biology, Faculty of Sciences and Letters, Artvin Coruh University, 08000, Artvin, Turkey

    Mehmet Demiralay

  3. Department of Molecular Biology and Genetics, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey

    Aykut Saglam

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  1. Rabiye Terzi
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  2. Ebru Kalaycıoglu
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  3. Mehmet Demiralay
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Correspondence to Rabiye Terzi.

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Communicated by W. Wang.

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Terzi, R., Kalaycıoglu, E., Demiralay, M. et al. Exogenous ascorbic acid mitigates accumulation of abscisic acid, proline and polyamine under osmotic stress in maize leaves. Acta Physiol Plant 37, 43 (2015). https://doi.org/10.1007/s11738-015-1792-0

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