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Superoxide dismutase activity in salt stressed wheat seedlings

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Abstract

We investigated the effect of salt stress on enzymatic activity of superoxide dismutase (SOD) isozymes in shoot and root tissues of salt tolerant and sensitive wheat (Triticum aestivum L. and Triticum durum Defs.) cultivars. Ten day old seedlings were subjected to 0.7 M NaCl stress for 3 and 5 days. Seedlings treated in the same manner without salt stress served as controls. Activity of SOD isozymes in root and shoot extracts was determined by activity staining of native polyacrylamide gels. In both shoot and root extracts of examined cultivars two isozymes of SOD, namely MnSOD and Cu/ZnSOD were identified. Cu/ZnSOD activity comprised 90 % of total SOD activity in both root and shoot tissues. Salt stress caused 1–1.5 fold increase in MnSOD activity of shoots in tolerant cultivars when compared with non-stressed controls. Under stress conditions, compared to controls all cultivars exhibited reduced MnSOD activity in root tissues. Cu/ZnSOD activity, on the other hand, was remarkably enhanced (3–4 fold) in root extracts of the tolerant cultivars, whereas it was reduced in the sensitive ones.

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Abbreviations

SOD:

Superoxide dismutase

EDTA:

Ethylenediaminetetraacetic acid

NBT:

Nitroblue tetrazolium

TEMED:

N′,N′,N′,N′-tetramethyl ethylene diamine

BEZOS:

Bezostaja

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Author notes

  1. Füsun (Inci) Eyidoğan

    Present address: Education Faculty, Baskent University, Ankara, Turkey

Authors and Affiliations

  1. Department of Biology, Middle East Technical University, 06531, Ankara, Turkey

    Füsun (Inci) Eyidoğan, Hüseyin Avni Öktem & Meral Yücel

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  1. Füsun (Inci) Eyidoğan
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  2. Hüseyin Avni Öktem
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  3. Meral Yücel
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Correspondence to Hüseyin Avni Öktem.

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(Inci) Eyidoğan, F., Öktem, H.A. & Yücel, M. Superoxide dismutase activity in salt stressed wheat seedlings. Acta Physiol Plant 25, 263–269 (2003). https://doi.org/10.1007/s11738-003-0007-2

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  • DOI: https://doi.org/10.1007/s11738-003-0007-2

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