Abstract
Background
To understand the role of ghrelin in the mechanism of action of laparoscopic sleeve gastrectomy (LSG), a prospective cohort case-control study to assess the expression of ghrelin-producing cells (GPC) in two groups of patients was designed.
Methods
Specimens of resected stomach from 26 obese patients who underwent LSG (group A), were compared by immunohistochemistry to control stomach samples from 26 non-obese patients (group B) resected for other pathologies or during autopsy; (GIST: 6 cases, inflammatory diseases: 4 cases, post-mortem autopsy cases with stomachs from healthy persons victims of traumatic accidents: 16 cases). Immunohistochemistry investigation was performed with the use of Ventana Benchmark ultra, anti-ghrelin antibody NOVUS, mouse monoclonal 2F4, diluted at 1:100.
Results
No significant difference in the expression of GPC number between group A and B was found (p = 0.87). No significant correlation between patients presenting a GPC number above (subgroup 1) or below (subgroup 2) the average, and EWL% changes, both at 1 and 6 years of follow-up, was recorded.
Conclusions
Our study has shown that the expression of GPC is similar in the stomach of obese and non-obese controls, being mostly influenced by the inflammatory status of the gastric mucosa. A variation in the preoperative number of GPC has not influenced the weight loss in patients who underwent LSG.
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Acknowledgments
The authors would like to acknowledge Prof. Maurizio Taglialatela for his precious help in drafting this manuscript.
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Contributions
Authors MM, MDA, GG, GP, and FPDA gave their substantial contributions to the conception or design of the work, while authors FDC, NV, AB, KDL, and SC dedicated to the acquisition, analysis, or interpretation of data for the work.
All authors:
• Were involved in drafting the work or revising it critically for important intellectual content;
• Gave final approval of the version to be published;
• Agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
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All LSG procedures performed by authors were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Ethical approval to this study was obtained from “Federico II” University institutional review board.
Conflict of Interest
The authors declare that they have no conflicts of interest.
Informed Consent
An informed consent to surgery was obtained from all patients who underwent a LSG procedure performed by anyone of the authors of this study during these years.
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Musella, M., Di Capua, F., D’Armiento, M. et al. No Difference in Ghrelin-Producing Cell Expression in Obese Versus Non-obese Stomach: a Prospective Histopathological Case-Control Study. OBES SURG 28, 3604–3610 (2018). https://doi.org/10.1007/s11695-018-3401-y
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DOI: https://doi.org/10.1007/s11695-018-3401-y