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Cell line derived from muscle of Gymnocypris przewalskii, a species of Schizothoracinae in Qinghai Lake, Qinghai–Tibet Plateau

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Abstract

Gymnocypris przewalskii (naked carp), a native teleost, plays an important role in the ecosystem of Qinghai Lake (altitude, 3.2 km) on the Qinghai–Tibet Plateau in China. We developed a new cell line from the muscle of G. przewalskii using the explant technique and named the cell line GPM. This cell line was maintained in DMEM medium (high glucose) supplemented with 15% fetal bovine serum (FBS). The cell line was successfully subcultured up to 32 passages and was authenticated by immunofluorescence assay, sequencing the mitochondrial cytochrome C oxidase subunit I (COI) and 16S rRNA genes, and by chromosome analysis. In the medium containing 15% FBS, the cell line could be passaged stably at 25 °C. The GPM cell line could express green fluorescent protein (GFP) with a CMV promoter with about 5% transfection efficiency. MTT tests showed that Clostridium botulinum toxin (BTX) was toxic to the cell line. The cell line could be successfully cryopreserved in liquid nitrogen with a revival efficiency of over 70%. This study demonstrated that the GPM cell line can be used as an important tool for understanding the physiological characteristics of G. przewalskii, and it can provide a resource for studying gene function and toxicological reactions in vitro.

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Acknowledgements

This work was financially supported by the National Natural Fund of China (31660745 and 31960741), The Central Guidance on Local Science and Technology Development Fund of Qinghai Province (2022ZY001), and the Independent project of State Key Laboratory of Plateau Ecology and Agriculture (2020-zz-01), Qinghai University. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

This work was financially supported by the National Natural Fund of China (31660745 and 31960741), The Central Guidance on Local Science and Technology Development Fund of Qinghai Province (2022ZY001), and the Independent project of State Key Laboratory of Plateau Ecology and Agriculture (2020-zz-01), Qinghai University.

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Authors and Affiliations

  1. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, 251 Ningda Road, Xining, 810016, People’s Republic of China

    Fulei Wei, Jian Liang, Miao Yue & Dingfan Xu

  2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People’s Republic of China

    Fulei Wei & Qiang Hua

  3. The Rescue and Rehabilitation Center of Naked Carps in Lake Qinghai, 83 Ningzhang Road, Xining, 810016, People’s Republic of China

    Wengen Tian, Luxian Yu & Zhaohui Feng

Authors
  1. Fulei Wei
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  2. Qiang Hua
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  3. Jian Liang
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  4. Miao Yue
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  5. Dingfan Xu
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  7. Luxian Yu
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  8. Zhaohui Feng
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Contributions

Fulei Wei: conceptualization, methodology, software, writing—original draft preparation, writing—reviewing and editing; Jian Liang: data curation, methodology, software; Miao Yue, Dingfan Xu: experimental assistance; Wengen Tian, Luxian Yu, Zhaohui Feng: artificial breeding and cultivation of experimental animals.

Corresponding author

Correspondence to Jian Liang.

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Ethics statement

All of the procedures described in this study complied with the relevant guidelines and regulations imposed by the Administration of Affairs Concerning Experimental Animals approved by the State Science and Technology Commission on October 31, 1988.

Competing interests

The authors declare no competing interests.

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Wei, F., Hua, Q., Liang, J. et al. Cell line derived from muscle of Gymnocypris przewalskii, a species of Schizothoracinae in Qinghai Lake, Qinghai–Tibet Plateau. In Vitro Cell.Dev.Biol.-Animal 58, 970–978 (2022). https://doi.org/10.1007/s11626-022-00729-z

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