Skip to main content

Advertisement

SpringerLink
Log in

Xian-Ling-Gu-Bao protects osteoporosis through promoting osteoblast differentiation by targeting miR-100-5p/KDM6B/RUNX2 axis

  • Report
  • Published:
In Vitro Cellular & Developmental Biology - Animal Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.

References

  • Cai N, Li C (2019) Silencing of LncRNA-ANCR promotes the osteogenesis of osteoblast cells in postmenopausal osteoporosis via targeting EZH2 and RUNX2. Yonsei Med J 60:751–759

    Article  CAS  Google Scholar 

  • Charbord P, Livne E, Gross G, Haupl T, Neves NM, Marie P, Bianco P, Jorgensen C (2011) Human bone marrow mesenchymal stem cells: a systematic reappraisal via the genostem experience. Stem Cell Rev Rep 7:32–42

    Article  CAS  Google Scholar 

  • Chen P, Lin C, Quan J, Lai Y, He T, Zhou L, Pan X, Wu X, Wang Y, Ni L, Yang S, Wang T, Lai Y (2017) Oncogenic miR-100-5p is associated with cellular viability, migration and apoptosis in renal cell carcinoma. Mol Med Rep 16:5023–5030

    Article  CAS  Google Scholar 

  • Coughlan T, Dockery F (2014) Osteoporosis and fracture risk in older people. Clin Med 14:187–191

    Article  Google Scholar 

  • Ecke TH, Stier K, Weickmann S, Zhao Z, Buckendahl L, Stephan C, Kilic E, Jung K (2017) miR-199a-3p and miR-214-3p improve the overall survival prediction of muscle-invasive bladder cancer patients after radical cystectomy. Cancer Med 6:2252–2262

    Article  CAS  Google Scholar 

  • Ensrud KE, Crandall CJ (2017) Osteoporosis. Ann Intern Med 167:Itc17–itc32

    Article  Google Scholar 

  • Eriksen EF, Diez-Perez A, Boonen S (2014) Update on long-term treatment with bisphosphonates for postmenopausal osteoporosis: a systematic review. Bone 58:126–135

    Article  CAS  Google Scholar 

  • Feng L, Xia B, Tian BF, Lu GB (2019) MiR-152 influences osteoporosis through regulation of osteoblast differentiation by targeting RICTOR. Pharm Biol 57:586–594

    Article  CAS  Google Scholar 

  • Fu YC, Zhao SR, Zhu BH, Guo SS, Wang XX (2019) MiRNA-27a-3p promotes osteogenic differentiation of human mesenchymal stem cells through targeting ATF3. Eur Rev Med Pharmacol Sci 23:73–80

    PubMed  Google Scholar 

  • Gan D, Xu X, Chen D, Feng P, Xu Z (2019) Network pharmacology-based pharmacological mechanism of the Chinese medicine Rhizoma drynariae against osteoporosis. Med Sci Monit 25:5700–5716

    Article  CAS  Google Scholar 

  • Ha H, Ho J, Shin S, Kim H, Koo S, Kim IH, Kim C (2003) Effects of Eucommiae cortex on osteoblast-like cell proliferation and osteoclast inhibition. Arch Pharm Res 26:929–936

    Article  CAS  Google Scholar 

  • Han Y, Zhang K, Hong Y, Wang J, Liu Q, Zhang Z, Xia H, Tang Y, Li T, Li L, Xue Y, Hong W (2018) miR-342-3p promotes osteogenic differentiation via targeting ATF3. FEBS Lett 592:4051–4065

    Article  CAS  Google Scholar 

  • He QL, Qin SY, Tao L, Ning HJ, Jiang HX (2019) Prognostic value and prospective molecular mechanism of miR-100-5p in hepatocellular carcinoma: a comprehensive study based on 1,258 samples. Oncol Lett 18:6126–6142

    CAS  PubMed  PubMed Central  Google Scholar 

  • Jakob M, Mattes LM, Kuffer S, Unger K, Hess J, Bertlich M, Haubner F, Ihler F, Canis M, Weiss BG (2019) MicroRNA expression patterns in oral squamous cell carcinoma: hsa-mir-99b-3p and hsa-mir-100-5p as novel prognostic markers for oral cancer. Head Neck-J Sci Spec 41:3499–3515

    Article  Google Scholar 

  • Kelch S, Balmayor ER (2017) miRNAs in bone tissue correlate to bone mineral density and circulating miRNAs are gender independent in osteoporotic patients. Sci Rep 7:15861

    Article  Google Scholar 

  • Komori T (2017) Roles of Runx2 in skeletal development. Adv Exp Med Biol 962:83–93

    Article  CAS  Google Scholar 

  • Komori T (2019) Regulation of proliferation, differentiation and functions of osteoblasts by Runx2. Int J Mol Sci 20(7):1694

    Article  CAS  Google Scholar 

  • Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T (1997) Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89:755–764

    Article  CAS  Google Scholar 

  • Lai Y, Kacal M, Kanony M, Stukan I, Jatta K, Kis L, Norberg E, Vakifahmetoglu-Norberg H, Lewensohn R, Hydbring P, Ekman S (2019) miR-100-5p confers resistance to ALK tyrosine kinase inhibitors crizotinib and lorlatinib in EML4-ALK positive NSCLC. Biochem Biophys Res Commun 511:260–265

  • Lai Z, Cao Y (2019) Plasma miR-200c-3p, miR-100-5p, and miR-1826 serve as potential diagnostic biomarkers for knee osteoarthritis: randomized controlled trials. Medicine 98:e18110

    Article  CAS  Google Scholar 

  • Li D, Tian Y, Yin C, Huai Y, Zhao Y, Su P, Wang X, Pei J, Zhang K, Yang C, Dang K, Jiang S, Miao Z, Li M, Hao Q, Zhang G, Qian A (2019) Silencing of lncRNA AK045490 promotes osteoblast differentiation and bone formation via beta-catenin/TCF1/Runx2 signaling axis. International journal of molecular sciences. Int J Mol Sci 20:6229

  • Li WD, Yan CP, Wu Y, Weng ZB, Yin FZ, Yang GM, Cai BC, Chen ZP (2014) Osteoblasts proliferation and differentiation stimulating activities of the main components of Fructus Psoraleae corylifoliae. Phytomedicine 21:400–405

    Article  CAS  Google Scholar 

  • Li ZR, Cheng LM, Wang KZ, Yang NP, Yang SH, He W, Wang YS, Wang ZM, Yang P, Liu XZ, Luo YZ, Sun W, Wang HT, Zheng LZ, Wang XL, Qin L (2018) Herbal Fufang Xian Ling Gu Bao prevents corticosteroid-induced osteonecrosis of the femoral head-a first multicentre, randomised, double-blind, placebo-controlled clinical trial. J Orthop Translat 12:36–44

    Article  Google Scholar 

  • Lin X, Li L, Wu S, Tian J, Zheng W (2019) Activation of GPR30 promotes osteogenic differentiation of MC3T3-E1 cells: an implication in osteoporosis. IUBMB Life 71:1751–1759

    Article  CAS  Google Scholar 

  • Lindsay BR, Olufade T, Bauer J, Babrowicz J, Hahn R (2016) Patient-reported barriers to osteoporosis therapy. Arch Osteoporos 11:19

    Article  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods 25:402–408

    Article  CAS  Google Scholar 

  • Matsuoka K, Park KA, Ito M, Ikeda K, Takeshita S (2014) Osteoclast-derived complement component 3a stimulates osteoblast differentiation. J Bone Miner Res 29:1522–1530

    Article  CAS  Google Scholar 

  • Nabavi N, Saidy NRN, Venalainen E, Haegert A, Parolia A, Xue H, Wang Y, Wu R, Dong X, Collins C, Crea F, Wang Y (2017) miR-100-5p inhibition induces apoptosis in dormant prostate cancer cells and prevents the emergence of castration-resistant prostate cancer. Sci Rep:7, 4079

  • Otto F, Thornell AP, Crompton T, Denzel A, Gilmour KC, Rosewell IR, Stamp GW, Beddington RS, Mundlos S, Olsen BR, Selby PB, Owen MJ (1997) Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell 89:765–771

    Article  CAS  Google Scholar 

  • Tomasevic-Todorovic S, Vazic A, Issaka A, Hanna F (2018) Comparative assessment of fracture risk among osteoporosis and osteopenia patients: a cross-sectional study. Open Access Rheumatol 10:61–66

    Article  Google Scholar 

  • Wang C, Meng H, Wang X, Zhao C, Peng J, Wang Y (2016a) Differentiation of bone marrow mesenchymal stem cells in osteoblasts and adipocytes and its role in treatment of osteoporosis. Med Sci Monit 22:226–233

    Article  CAS  Google Scholar 

  • Wang L, Li Y, Guo Y, Ma R, Fu M, Niu J, Gao S, Zhang D (2016b) Herba Epimedii: an ancient Chinese herbal medicine in the prevention and treatment of osteoporosis. Curr Pharm Des 22:328–349

    Article  CAS  Google Scholar 

  • Wang Q, Li Y, Zhang Y, Ma L, Lin L, Meng J, Jiang L, Wang L, Zhou P, Zhang Y (2017) LncRNA MEG3 inhibited osteogenic differentiation of bone marrow mesenchymal stem cells from postmenopausal osteoporosis by targeting miR-133a-3p. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. Biomed Pharmacother 89:1178–1186

    Article  CAS  Google Scholar 

  • Wu H, Zhong Q, Wang J, Wang M, Fang F, Xia Z, Zhong R, Huang H, Ke Z, Wei Y, Feng L, Shi Z, Sun E, Song J, Jia X (2017) Beneficial effects and toxicity studies of Xian-ling-gu-bao on bone metabolism in ovariectomized rats. Front Pharmacol 8:273

    Article  Google Scholar 

  • Wu W, Wang T, Sun B, Liu D, Lin Z, Miao Y, Wang C, Geng X, Li B (2019) Xian-Ling-Gu-Bao induced inflammatory stress rat liver injury: inflammatory and oxidative stress playing important roles. J Ethnopharmacol 239:111910

    Article  CAS  Google Scholar 

  • Wu X, Li Z, Yang Z, Zheng C, Jing J, Chen Y, Ye X, Lian X, Qiu W, Yang F, Tang J, Xiao J, Liu M, Luo J (2012) Caffeic acid 3,4-dihydroxy-phenethyl ester suppresses receptor activator of NF-kappaB ligand-induced osteoclastogenesis and prevents ovariectomy-induced bone loss through inhibition of mitogen-activated protein kinase/activator protein 1 and Ca2+-nuclear factor of activated T-cells cytoplasmic 1 signaling pathways. J Bone Miner Res 27:1298–1308

    Article  CAS  Google Scholar 

  • Yang D, Okamura H, Nakashima Y, Haneji T (2013) Histone demethylase Jmjd3 regulates osteoblast differentiation via transcription factors Runx2 and osterix. J Biol Chem 288:33530–33541

    Article  CAS  Google Scholar 

  • Yasui T, Hirose J, Tsutsumi S, Nakamura K, Aburatani H, Tanaka S (2011) Epigenetic regulation of osteoclast differentiation: possible involvement of Jmjd3 in the histone demethylation of Nfatc1. J Bone Miner Res 26:2665–2671

    Article  CAS  Google Scholar 

  • Zhang ND, Han T, Huang BK, Rahman K, Jiang YP, Xu HT, Qin LP, Xin HL, Zhang QY, Li YM (2016) Traditional Chinese medicine formulas for the treatment of osteoporosis: implication for antiosteoporotic drug discovery. J Ethnopharmacol 189:61–80

    Article  Google Scholar 

  • Zhou L, Song HY, Gao LL, Yang LY, Mu S, Fu Q (2019) MicroRNA1005p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21. Int J Mol Med 43:727–738

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Natural Science Foundation of China (81774339), Traditional Chinese Medicine Bureau of Guangdong Province (20191066), and Major discipline research projects of Guangzhou University of Chinese Medicine in 2020 (XK2019012).

Author information

Authors and Affiliations

  1. Guangzhou University of Chinese Medicine, Guangzhou, 510006, China

    Liang Ai

  2. Department of Traditional Chinese Medicine, Sun Yat-Sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510000, China

    Liang Ai, Weimin Yi, Liudan Chen & Qihui Huang

  3. Department of Orthopedics, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

    Haibin Wang

Authors
  1. Liang Ai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Weimin Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liudan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Haibin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Qihui Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Haibin Wang or Qihui Huang.

Ethics declarations

Conflict of interest

We declare that we have no conflict of interest.

Additional information

Editor: Tetsuji Okamoto

Supplementary Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ai, L., Yi, W., Chen, L. et al. Xian-Ling-Gu-Bao protects osteoporosis through promoting osteoblast differentiation by targeting miR-100-5p/KDM6B/RUNX2 axis. In Vitro Cell.Dev.Biol.-Animal 57, 3–9 (2021). https://doi.org/10.1007/s11626-020-00530-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11626-020-00530-w

Search

Navigation