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Investigating the Antimicrobial, Antioxidant, and Anticancer Effects of Elettaria cardamomum Seed Extract Conjugated to Green Synthesized Silver Nanoparticles by Laser Ablation

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Abstract

To synthesize silver nanoparticles suspended in deionized water, Elettaria cardamomum (Car) seed extract and Car@Ag nanoparticles were prepared in three steps. The first used a hot aqueous extract of Elettaria cardamomum seed, and then, the second used pulsed laser ablation technique (PLAL) by Nd:YAG laser (λ = 1064 nm wavelength) at laser energy of 400 mJ, and the number of pulses was 500 pulses and third step for the synthesis of creating a non-conjugated Elettaria cardamomum extract. Ultraviolet visible (UV–Vis) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) were used to investigate the optical, structural, and morphological characterizations of synthesized Car@AgNPs. The nanoparticles were spherical in shape, and a size distribution exhibited a mean size of 21 nm, according to the TEM findings. AgNPs and Car@AgNPs have free radical scavenging activity in the DPPH assay. Additionally, Car@AgNPs were examined in vitro for zones of inhibition against pathogenic bacteria like Escherichia coli and Staphylococcus aureus. The inhibition rate of Escherichia coli was higher than that observed in Staphylococcus aureus. Finally, Car@AgNPs demonstrated a potential cytotoxicity agent breast cancer (MCF-7) cell line that was evaluated by MTT assay.

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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Yadav SK, Yadav RD, Tabassum H et al (2023) Recent Developments in nanotechnology-based biosensors for the diagnosis of coronavirus. Plasmonics 18:955–969. https://doi.org/10.1007/s11468-023-01822-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Mohammed HA et al (2023) Solid lipid nanoparticles for targeted natural and synthetic drugs delivery in high-incidence cancers, and other diseases: roles of preparation methods, lipid composition, transitional stability, and release profiles in nanocarriers’ development. Nanotechnol Rev 12(1):20220517. https://doi.org/10.1515/ntrev-2022-0517

  3. Khane Y, Benouis K, Albukhaty S, Sulaiman GM, Abomughaid MM, Al Ali A, Aouf D, Fenniche F, Khane S, Chaibi W et al (2013) Green synthesis of silver nanoparticles using aqueous Citrus limon zest extract: characterization and evaluation of their antioxidant and antimicrobial properties. Nanomaterials 2022:12. https://doi.org/10.3390/nano12122013

    Article  CAS  Google Scholar 

  4. Alyamani AA, Albukhaty S, Aloufi S, AlMalki FA, Al-Karagoly H, Sulaiman GM (2021) Green fabrication of zinc oxide nanoparticles using Phlomis leaf extract: characterization and in vitro evaluation of cytotoxicity and antibacterial properties. Molecules 26:6140. https://doi.org/10.3390/molecules26206140

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Al-Musawi S, Albukhaty S, Al-Karagoly H, Almalki F (2021) Design and synthesis of multi-functional superparamagnetic core-gold shell nanoparticles coated with chitosan and folate for targeted antitumor therapy. Nanomaterials 11:32. https://doi.org/10.3390/nano11010032

    Article  CAS  Google Scholar 

  6. Safat S, Buazar F, Albukhaty S et al (2021) Enhanced sunlight photocatalytic activity and biosafety of marine-driven synthesized cerium oxide nanoparticles. Sci Rep 11:14734. https://doi.org/10.1038/s41598-021-94327-w

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Albukhaty, S., Naderi-Manesh H, Tiraihi T, Sakhi Jabir M (2018) Poly-l-lysine-coated superparamagnetic nanoparticles: a novel method for the transfection of pro-BDNF into neural stem cells. Artif Cells Nanomed Biotechnol 46:S125–SS32. https://doi.org/10.1080/21691401.2018.1489272

  8. Mahmood RI, Kadhim AA, Ibraheem S et al (2022) Biosynthesis of copper oxide nanoparticles mediated Annona muricata as cytotoxic and apoptosis inducer factor in breast cancer cell lines. Sci Rep 12:16165. https://doi.org/10.1038/s41598-022-20360-y

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Xu L, Wang Y-Y, Huang J, Chen C-Y, Wang Z-X, Xie H (2020) Silver nanoparticles: synthesis, medical applications and biosafety. Theranostics 10:8996

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Saratale GD, Saratale RG, Benelli G, Kumar G, Pugazhendhi A, Kim D-S et al (2017) Anti-diabetic potential of silver nanoparticles synthesized with Argyreia nervosa leaf extract high synergistic antibacterial activity with standard antibiotics against foodborne bacteria. J Clust Sci 28:1709–1727

    Article  CAS  Google Scholar 

  11. Alzubaidi AK, Al-Kaabi WJ, Ali AA, Albukhaty S, Al-Karagoly H, Sulaiman GM, Asiri M, Khane Y (2023) Green synthesis and characterization of silver nanoparticles using flaxseed extract and evaluation of their antibacterial and antioxidant activities. Appl Sci 13:2182. https://doi.org/10.3390/app13042182

    Article  CAS  Google Scholar 

  12. Prasher P, Sharma M, Mudila H, Gupta G, Sharma AK, Kumar D, Bakshi HA, Negi P, Kapoor DN, Chellappan DK et al (2020) Emerging trends in clinical implications of bio-conjugated silver nanoparticles in drug delivery. Colloids Interface Sci Commun 35:100244

    Article  CAS  Google Scholar 

  13. Li G, Chen AJ, Chen XY, Li XL, Gao WW, Branch Y, Union P (2010) First report of Amomum villosum (cardamom) leaf lesion caused by Pyricularia costina in China. New Dis Rep 22:2

    Article  CAS  Google Scholar 

  14. Soshnikova V, Kim YJ, Singh P, Huo Y, Markus J, Ahn S, Castro-Aceituno V, Kang J, Chokkalingam M, Mathiyalagan R et al (2018) Cardamom fruits as a green resource for facile synthesis of gold and silver nanoparticles and their biological applications. Artif Cells Nanomed Biotechnol 46:108–117

    Article  CAS  PubMed  Google Scholar 

  15. Nurcholis W, Alfadzrin R, Izzati N, Arianti R, Vinnai BÁ, Sabri F, Kristóf E, Artika IM (2022) Effects of methods and durations of extraction on total flavonoid and phenolic contents and antioxidant activity of Java cardamom (Amomum compactum Soland Ex Maton) Fruit. Plants 11:2221. https://doi.org/10.3390/plants11172221

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Yahyazadeh R, Rahbardar MG, Razavi BM, Karimi G, Hosseinzadeh H (2021) The effect of Elettaria cardamomum (cardamom) on the metabolic syndrome: narrative review. Iran J Basic Med Sci 24(11):1462

    PubMed  PubMed Central  Google Scholar 

  17. Ashokumar K, Murugan M, Dhanya MK, Warkentin TD (2020) Botany, Traditional uses, phytochemistry and biological activities of cardamom [Elettaria Cardamomum (L.) Maton]—a critical review. J. Ethnopharmacol 246:112244

  18. Yugay Y, Rusapetova T, Mashtalyar D, Grigorchuk V, Vasyutkina E, Kudinova O, Zenkina K, Trifuntova I, Karabtsov A, Ivanov V (2021) Biomimetic synthesis of functional silver nanoparticles using hairy roots of Panax ginseng for wheat pathogenic fungi treatment. Colloids Surf B Biointerfaces 207:112031

    Article  CAS  PubMed  Google Scholar 

  19. Abbai R, Mathiyalagan R, Markus J, Kim YJ, Wang C, Singh P, Ahn S, Farh MEA, Yang DC (2016) Green synthesis of multifunctional silver and gold nanoparticles from the oriental herbal adaptogen: Siberian ginseng. Int J Nanomedicine 11:3131

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Adil M, Khan T, Aasim M, Khan AA, Ashraf M (2019) Evaluation of the antibacterial potential of silver nanoparticles synthesized through the interaction of antibiotic and aqueous callus extract of Fagonia indica. AMB Express 9(1):1–12

    Article  CAS  Google Scholar 

  21. Hussein HH, Maih RK, Younis AH, Hassoun BA (2017) Evaluation of antifungal activity of hot water extract on Elettaria cardamomum and Cinnamomum sp. against some opportunism fungi. Al-Kufa Univ J Biol 2:324–331

  22. Jawad KH, Hasson B (2021) Developing strategy for a successful antioxidant, anticancer activity via an improved method prepared to porous silicon nanoparticles. J Appl Sci Nanotechnol 3(11):1–11. https://doi.org/10.53293/jasn.2021.3890.1054

    Article  Google Scholar 

  23. Al-Anee RS, Sulaiman GM, Al-Sammarrae KW, Napolitano G, Bagnati R, Lania L, Passoni A, Majello B (2015) Chemical characterization, antioxidant and cytotoxic activities of the methanolic extract of Hymenocrater longiflorus grown in Iraq. Zeitschrift Für Naturforschung C 70(9–10):227–235

    Article  CAS  Google Scholar 

  24. Jawad KH, Hasoon BA, Ismail RA, Shaker SS (2022) Preparation of copper oxide nanosheets by pulsed laser ablation in liquid for anticancer, antioxidant, and antibacterial activities. J Indian Chem Soc 99(11):100773

    Article  CAS  Google Scholar 

  25. Alhujaily M, Albukhaty S, Yusuf M, Mohammed MKA, Sulaiman GM, Al-Karagoly H, Alyamani AA, Albaqami J, AlMalki FA (2022) Recent advances in plant-mediated zinc oxide nanoparticles with their significant biomedical properties. Bioengineering 9:541. https://doi.org/10.3390/bioengineering9100541

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Tran QH, Le AT (2013) Silver nanoparticles : synthesis, properties, toxicology, applications and perspectives. Advances in Natural Sciences: Nanoscience and Nanotechnology 4(3):033001

    CAS  Google Scholar 

  27. Soshnikova V, Kim YJ, Singh P, Huo Y, Markus J, Ahn S, Castro-Aceituno V, Kang J, Chokkalingam M, Mathiyalagan R, Yang DC (2018) Cardamom fruits as a green resource for facile synthesis of gold and silver nanoparticles and their biological applications. Artificial Cells, Nanomedicine, and Biotechnology 46(1):108–111

    Article  CAS  PubMed  Google Scholar 

  28. Fahmy HM, Mosleh AM, Abd Elghany A, Shams-Eldin E, Serea ESA, Alia SA, Shalan AE (2019) Coated silver nanoparticles: synthesis, cytotoxicity, and optical properties. RSC Adv 9:20118–20136

  29. Peng F, Jeong S, Ho A, Evans CL (2021) Recent progress in plasmonic nanoparticle-based biomarker detection and cytometry for the study of central nervous system disorders. Cytometry A 99(11):1067–1078

    Article  CAS  PubMed  Google Scholar 

  30. Kaur A, Kumar R (2019) Enhanced bactericidal efficacy of polymer stabilized silver nanoparticles in conjugation with different classes of antibiotics. RSC Adv 9:1095–1105

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Muzamil M, Khalid N, Aziz MD, Abbas SA (2014) Synthesis of silver nanoparticles by silver salt reduction and its characterization. In IOPConference Series: Materials Science and Engineering 60:012034

    CAS  Google Scholar 

  32. Konop M, Damps T, Misicka A, Rudnicka L (2016) Certain aspects of silver and silver nanoparticles in wound care: a minireview. J Nanomater 2016:1–10

    Article  Google Scholar 

  33. Al-Majedy YK, Ibraheem HH, Issa AA, Jabir MS, Hasoon BA, Al-Shmgani HS, Sulaiman GM (2023) Synthesis, biomedical activities, and molecular docking study of novel chromone derivatives. J Mol Struct 1295(1):136647

    Google Scholar 

  34. Jamil B, Abbasi R, Abbasi S, Imran M, Khan SU, Ihsan A, Javed S, Bokhari H, Imran M (2016) Encapsulation of cardamom essential oil in chitosan nano-composites: in vitro efficacy on antibiotic-resistant bacterial pathogens and cytotoxicity studies. Frontiers Microbiol 7(573518):1580. https://doi.org/10.3389/fmicb.2016.01580

    Article  Google Scholar 

  35. Lü JM, Lin PH, Yao Q, Chen C (2010) Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med 14:840–860

    Article  PubMed  Google Scholar 

  36. Priya RS, Geetha D, Ramesh P (2016) Antioxidant activity of chemically synthesized AgNPs and biosynthesized Pongamia pinnata leaf extract mediated AgNPs—a comparative study. Ecotoxicol Environ Saf 134:308–318

    Article  CAS  PubMed  Google Scholar 

  37. Choi CW, Kim SC, Hwang SS, Choi BK, Ahn HJ, Lee MY, Park SH, Kim SK (2002) Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci 163:1161–1168

    Article  CAS  Google Scholar 

  38. Rhyaf A, Naji H, Al-Karagoly H, Albukhaty S, Sulaiman GM, Alshammari AAA, Mohammed HA, Jabir M, Khan RA (2023) In vitro and in vivo functional viability, and biocompatibility evaluation of bovine serum albumin-ingrained microemulsion: a model based on sesame oil as the payload for developing an efficient drug delivery platform. Pharmaceuticals 16:582. https://doi.org/10.3390/ph16040582

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Al-Khedhairy AA, Wahab R (2022) Silver nanoparticles: an instantaneous solution for anticancer activity against human liver (HepG2) and breast (MCF-7) cancer cells. Metals 12:148. https://doi.org/10.3390/met12010148

    Article  CAS  Google Scholar 

  40. Rajivgandhi GN, Chackaravarthi G, Ramachandran G, Chelliah CK, Maruthupandy M, Alharbi MS, Alharbi NS, Khaled JM, Li WJ (2022) Morphological damage and increased ROS production of biosynthesized silver nanoparticle against MCF-7 breast cancer cells through in vitro approaches. Journal of King Saud University-Science 34(2):101795

    Article  Google Scholar 

  41. Selvi BCG, Madhavan J, Santhanam A (2016) Cytotoxic effect of silver nanoparticles synthesized from Padina tetrastromatica on breast cancer cell line. Advances in Natural Sciences: Nanoscience and Nanotechnology 7(3):035015

    Google Scholar 

  42. Sangour MH, Ali IM, Atwan ZW, Al Ali AAALA (2021) Effect of Ag nanoparticles on viability of MCF-7 and Vero cell lines and gene expression of apoptotic genes. Egypt J Med Human Gen 22:1–11

  43. Mukherjee SG, O’Claonadh N, Casey A, Chambers G (2012) Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines. Toxicol in Vitro 26:238–251

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The authors extend their appreciation to Ondokuz Mayis University, Turkey, the University of Technology, Baghdad, Iraq, and the University of Misan, Maysan, Iraq, for their technical support

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Authors and Affiliations

  1. Nanoscience and Nanotechnology, Ondokuz Mayis University, Samsun, Turkey

    Alya A. Mohammed & Sinem Çevik

  2. Department of Laser & Optoelectronics Engineering, University of Technology, Baghdad, Iraq

    Kareem H. Jawad

  3. Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, Iraq

    Ghassan M. Sulaiman

  4. Department of Chemistry, College of Science, University of Misan, Amarah, Maysan, 62001, Iraq

    Salim Albukhaty

  5. College of Medicine, University of Warith Al-Anbiyaa, Karbala, Iraq

    Salim Albukhaty

  6. Department of Physics, Saveetha School of Engineering, SIMATS, Thandalam-602 105, Chennai, 602 105, India

    P. Sasikumar

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Contributions

Conceptualization and methodology; Alya A. Mohammed, and Kareem H. Jawad.; Formal analysis, Sinem Çevik; Investigation and data curation Salim Albukhaty, and Ghassan M. Sulaiman.; validation P. Sasikumar; visualization Alya A. Mohammed; Original draft preparation Kareem H. Jawad; writing—review and editing, Sinem Çevik.; Supervision, Salim Albukhaty, and Ghassan M. Sulaiman. All authors gave approval to the final version of the manuscript.

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Correspondence to Ghassan M. Sulaiman or Salim Albukhaty.

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Mohammed, A.A., Jawad, K.H., Çevik, S. et al. Investigating the Antimicrobial, Antioxidant, and Anticancer Effects of Elettaria cardamomum Seed Extract Conjugated to Green Synthesized Silver Nanoparticles by Laser Ablation. Plasmonics (2023). https://doi.org/10.1007/s11468-023-02067-6

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