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Control of Fusarium and nematodes by entomopathogenic fungi for organic production of Zingiber officinale

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Abstract

Ginger (genus Zingiber) is widely used as a spice and a medicinal herb worldwide and is the major ingredient of traditional local drinks such as jamu in Southeast Asia. Because ginger is frequently consumed, there is an increasing interest in organic ginger production without the use of synthetic agrochemicals. Recent studies have reported that certain kinds of entomopathogenic fungi (EPF) can establish endophytic- or mycorrhiza-like relationships with plants, thereby promoting plant growth and health, in addition to their typical role in crop protection as biological control agents. In this study, we explored the possibility of non-entomopathogenic effects of EPF Beauveria bassiana and Cordyceps fumosorosea on ginger plants (Zingiber officinale) via antagonism with Fusarium oxysporum or the parasitic nematode Meloidogyne incognita. The two EPF negatively affected the growth of F. oxysporum and survival of M. incognita in vitro. The application of EPF did not have any negative effect on the growth of ginger plants. Soil chemical properties were not different between the plots with or without EPF application, while the diversity of soil bacteria was observed to increase on application of EPF. At least C. fumosorosea appeared to persist in soil during the period of ginger cultivation. Thus, these EPF are potentially useful tools for producing chemical-free ginger.

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taken from each container at the onset of ginger cultivation and after harvest and were used for DNA extraction in May and November. b Principal component analysis of the bacterial profiles in soils with or without fungal application. Bb 782: soil in the container treated with B. bassiana 782; Cf K3: soil treated with C. fumosorosea K3; chitin: soil treated with chitin; and control: soil without amendment

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Acknowledgements

The authors deeply thank Dr. Daigo Aiuchi (Obihiro University, Japan) for providing the recipe of oxgall medium and Junko Motokawa for helping with the analysis of the soil chemicals.

Funding

This work was supported by the Research Institute for Sustainable Humanosphere, Kyoto University (Grant number 2019 mission 1).

Author information

Author notes

  1. Minoru Kubo

    Present address: Center for Digital Green-innovation, Nara Institute of Science and Technology, Nara, 630-0192, Japan

  2. Chihiro Furumizu

    Present address: Graduate School of Medicine , Akita University, Akita, 010-8543, Japan

Authors and Affiliations

  1. Research Institute for Sustainable Humanosphere, Kyoto University, Uji, 611-0011, Japan

    Aya Yanagawa & Akifumi Sugiyama

  2. Research Center for Biomaterials, National Research and Innovation Agency (BRIN), Jakarta, 16911, Indonesia

    Ni Putu Ratna Ayu Krishanti

  3. Salsabila Library, Cipaku, Bogor, 16133, Indonesia

    Emiria Chrysanti

  4. Research Center for Biology, National Research and Innovation Agency - BRIN Cibinong Science Center, Cibinong, Bogor, 16911, Indonesia

    Safendrri Komara Ragamustari

  5. Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555, Japan

    Minoru Kubo, Chihiro Furumizu & Shinichiro Sawa

  6. University of California Cooperative Extension, San Luis Obispo, CA, 93401, USA

    Surendra K. Dara

  7. Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Masaru Kobayashi

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  1. Aya Yanagawa
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Correspondence to Masaru Kobayashi.

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Yanagawa, A., Krishanti, N.P.R.A., Sugiyama, A. et al. Control of Fusarium and nematodes by entomopathogenic fungi for organic production of Zingiber officinale. J Nat Med 76, 291–297 (2022). https://doi.org/10.1007/s11418-021-01572-4

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  • DOI: https://doi.org/10.1007/s11418-021-01572-4

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