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Application of a proton quantitative nuclear magnetic resonance spectroscopy method for the determination of actinodaphnine in Illigera aromatica and Illigera henryi

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Abstract

Illigera aromatica S. Z. Huang et S. L. Mo and Illigera henryi W. W. Sm., belonging to the genus Illigera (Hernandiaceae), are used as herbal medicines for promoting blood circulation and treating tuberculosis. Actinodaphnine, the major bioactive alkaloid, plays an important role in the quality controls of the herbs. In the present study, a rapid, simple, accurate, and precise proton quantitative nuclear magnetic resonance (1H-qNMR) method was developed to determine the content of actinodaphnine in I. aromatica and I. henryi. DMSO-d6 enabled satisfactory separation of the signals to be integrated in 1H NMR spectrum. 1,4-Dinitrobenzene was selected as an internal standard. The limits of determination and quantitation were 0.005 and 0.038 mg/mL, respectively. This work implied that 1H-qNMR represents a feasible alternative to HPLC-based methods for quantitation of actinodaphnine in I. aromatica and I. henryi and is suitable for the quality control of I. aromatica and I. henryi.

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Acknowledgements

This work was financially supported by a Yunnan Local Colleges Applied Basic Research Project (No. 2017FH001-092), a grant from the Science and Technology Project of Yunnan Provincial Department of Science and Technology (No. 2018FD081), a grant from the Shanghai Key Laboratory of Rare Earth Functional Materials, and a grant from the Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province.

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Authors and Affiliations

  1. College of Chemistry and Environmental Science, Qujing Normal University, Qujing, 655011, People’s Republic of China

    Jian-Wei Dong, Xue-Jiao Li, Jun-You Shi & Kai-Quan Liu

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  1. Jian-Wei Dong
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  2. Xue-Jiao Li
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  4. Kai-Quan Liu
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Correspondence to Jian-Wei Dong.

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Dong, JW., Li, XJ., Shi, JY. et al. Application of a proton quantitative nuclear magnetic resonance spectroscopy method for the determination of actinodaphnine in Illigera aromatica and Illigera henryi. J Nat Med 73, 312–317 (2019). https://doi.org/10.1007/s11418-018-1264-0

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