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l-Carnitine and vitamin E ameliorate cardiotoxicity induced by tilmicosin in rats

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Abstract

The present study aimed to investigate the possible mitigating effect of l-carnitine (LC) and/or α-tocopherol (Vit. E) administration against tilmicosin (TIL)-induced cardiotoxicity in rats. Fifty-six male albino rats were divided into seven groups according to LC, Vit. E, and/or TIL administration. Control, LC, and Vit. E groups were given saline, 150 mg LC/kg body weight (BW)/day and 100 mg Vit. E/kg BW/day, respectively, orally once daily for 15 days. The TIL group was administered saline orally once daily for 15 days and a single dose of TIL (75 mg/kg BW) subcutaneously (SC) on day 14 from the starting of the experimental period (15 days). The TIL-LC, TIL-Vit. E, and TIL-LC-Vit. E groups received 150 mg LC/kg BW/day, 100 mg Vit. E/kg BW/day, and 150 mg LC/kg BW pulse 100 mg Vit. E/kg BW, respectively, orally once daily for 15 days with TIL as described above. The results revealed that the administration of TIL significantly (P ≤ 0.05) raised serum activities of heart injury indicators, lactate dehydrogenase (LDH), creatine kinase (CK), and CK-MB with substantial increase (P ≤ 0.05) in the cardiac contents of malondialdehyde (MDA) and decreased in antioxidants. The pathological changes appeared in the form of necrotic muscle fibers and massive inflammatory cellular infiltrations in the cardiac muscle and increased the caspase-3 immunohistochemical expression in the heart tissues as well. These changes were ameliorated by LC and/or Vit. E administration. In conclusion, supplementation of LC and/or Vit. E ameliorated the cardiotoxicity of the TIL SC injection in the rat.

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Acknowledgments

We would like to thank Dr. Amr El Kelish, Dep. of Biology, Faculty of Science, Suez Canal University, Egypt, for her valuable support.

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Authors and Affiliations

  1. Pharmacology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Qalioubeya, 13736, Egypt

    Mohamed Aboubakr & Faten Elsayd

  2. Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Ahmed Soliman

  3. Biochemistry Department, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt

    Sabreen Ezzat Fadl

  4. Anatomy Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Qalioubeya, 13736, Egypt

    Anwar El-Shafey

  5. Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt

    Ehab Yahya Abdelhiee

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  1. Mohamed Aboubakr
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Correspondence to Ehab Yahya Abdelhiee.

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Rats were treated in accordance with the Guidelines for Animal Experimentation of the Ethics Review Committee of the Faculty of Veterinary Medicine, Benha University, Egypt (Approval No. 201912).

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Aboubakr, M., Elsayd, F., Soliman, A. et al. l-Carnitine and vitamin E ameliorate cardiotoxicity induced by tilmicosin in rats. Environ Sci Pollut Res 27, 23026–23034 (2020). https://doi.org/10.1007/s11356-020-08919-6

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