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Experimental simulation of somatic and germ cell genotoxicity in male Mus musculus fed extracts of lead contaminated Pleurotus ostreatus (white rot fungi)

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Abstract

Exposure to lead (Pb) is a major risk factor in reproductive toxicity, somatic, and germ cell genotoxicity. Exposure via deteriorating Pb paints and contaminated air, soil, and water had been the primary routes. However, with increasing reports of Pb accumulation in mushrooms and other food items may increase the etiology of Pb poisoning. The study herein investigated somatic genotoxicity and reproductive abnormalities in mice fed extracts of Pb-contaminated Pleurotus ostreatus. Male mice were fed aqueous extracts of P. ostreatus cultivated in 0, 10, 20, 100, 200, 500, and 1000 mg/L of Pb-contaminated rice straw for 35 days. Testes were analyzed for Pb accumulation, histopathology and relative weight gain, caudal epididymis for abnormal sperm morphology, and bone marrow for micronucleus test. Concentration-related significant increase in Pb accumulation was observed in P. ostreatus and testes of exposed mice. Decrease testicular weight, congestion of blood vessels, necrosis, and disorganization of the seminiferous tubules were observed in treated mice. In addition, fold increase of 2.78, 3.39, 6.67, 7.21, 9.63, and 9.70 in abnormal sperm morphology in accordance with the Pb concentrations respectively, confirmed reproductive toxicity. Significant increase in micronucleated polychromatic (PCE) and normochromatic (NCE) erythrocytes and concentration-related decrease PCE-NCE ratio in the bone marrow of treated mice suggest genome instability. Pb-contaminated P. ostreatus increased somatic and germ cell genotoxicity in mice. This may predispose the mice to genetic related syndromes and reproductive syndromes. It further suggests caution in the consumption of metal laden wild mushrooms and crop plants.

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Authors and Affiliations

  1. Department of Zoology, Ecology and Environmental Biology Unit, University of Ibadan, Ibadan, Nigeria

    Adetola M. Olatunji-Ojo

  2. Department of Animal and Environmental Biology, Faculty of Science, Environmental Biology Unit, Adekunle Ajasin University, Akungba, Nigeria

    Adetola M. Olatunji-Ojo

  3. Department of Zoology, Cell Biology and Genetics Unit, University of Ibadan, Ibadan, Nigeria

    Chibuisi G. Alimba & Adekunle A. Bakare

  4. Leibniz Research Centre for Working Environment and Human Factors (IfADo), Technical University of Dortmund, 44139, Dortmund, Germany

    Chibuisi G. Alimba

  5. Department of Botany, Mushroom Biotechnology Unit, University of Ibadan, Ibadan, Nigeria

    Clementina O. Adenipekun

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  1. Adetola M. Olatunji-Ojo
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  2. Chibuisi G. Alimba
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  3. Clementina O. Adenipekun
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  4. Adekunle A. Bakare
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Correspondence to Chibuisi G. Alimba.

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Guide for care and use of laboratory animals in accordance with the US National Institute of Health (NIH Publication No. 85-23, revised in 1996) (Gad 2007) was strictly adhered.

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Olatunji-Ojo, A.M., Alimba, C.G., Adenipekun, C.O. et al. Experimental simulation of somatic and germ cell genotoxicity in male Mus musculus fed extracts of lead contaminated Pleurotus ostreatus (white rot fungi). Environ Sci Pollut Res 27, 19754–19763 (2020). https://doi.org/10.1007/s11356-020-08494-w

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  • DOI: https://doi.org/10.1007/s11356-020-08494-w

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