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Lower cadmium accumulation and higher antioxidative capacity in edible parts of Brassica campestris L. seedlings applied with glutathione under cadmium toxicity

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Abstract

Glutathione (GSH) is involved in not only plant developmental processes but also plant responses to abiotic stresses. A hydroponic experiment was performed to explore the protective roles of exogenous GSH in mitigating cadmium (Cd) stress in Brassica campestris L. seedlings by analyzing the morphological and physiological parameters. Results showed that Cd caused severe growth inhibition and Cd accumulation. However, application of GSH significantly mitigated toxic symptoms induced by Cd, including the improvement of the photosynthesis-, plant growth-, and root morphology-related parameters in seedlings under Cd stress. These responses were associated with a striking increase in activities of representative antioxidative enzymes and contents of corresponding non-enzymatic antioxidants. In vivo imaging of O2.- and H2O2, and the detection of lipid peroxidation further demonstrated that increased ability by GSH for Brassica campestris L. seedlings to endure Cd stress was consistent with a striking elevation of ratios of reduced to oxidized glutathione (GSH/GSSG) and ascorbic acid to dehydroascorbic acid (AsA/DHA). Additionally, GSH application increased Cd retained in roots, thus significantly decreased its translocation from root to shoot, ultimately decreased Cd accumulation in shoots. Taken together, our results proved evidence for GSH in ameliorating Cd toxicity via reducing Cd accumulation in shoots and increasing oxidation resistance. Accordingly, application of GSH could be a high-efficiency and promising strategy to decrease Cd concentration in edible parts of Brassica campestris L. in agricultural production.

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Funding

This work was financially supported by the National Key Research and Development Program (2016YFD0800300) and China Agriculture Research System (CARS-23-A03).

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  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, China

    Yifan Huang, Zhengbo Zhu, Xue Wu, Zili Liu, Yahua Chen, Nana Su & Jin Cui

  2. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China

    Jianwen Zou

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  1. Yifan Huang
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  2. Zhengbo Zhu
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Huang, Y., Zhu, Z., Wu, X. et al. Lower cadmium accumulation and higher antioxidative capacity in edible parts of Brassica campestris L. seedlings applied with glutathione under cadmium toxicity. Environ Sci Pollut Res 26, 13235–13245 (2019). https://doi.org/10.1007/s11356-019-04745-7

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