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The protective effects of Nile tilapia (Oreochromis niloticus) scale collagen hydrolysate against oxidative stress induced by tributyltin in HepG2 cells

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Abstract

Oxidative stress is regarded as one of the most important factors associated with many diseases, such as atherosclerosis, cancer, and diabetes. Various chemicals are released into the environment, causing environmental pollution. Importantly, many of them may cause damage to organisms through oxidative stress. In this work, we investigated the possible protective effects of Nile tilapia (Oreochromis niloticus) scale collagen hydrolysate (TSCH) (molecular weight approximately 4 kDa) against tributyltin (TBT)-induced oxidative stress in vitro. The results showed that pretreatment with TSCH protected against decreases in cell viability and changes in cell morphology in HepG2 cells exposed to TBT. Treatment with TSCH reduced the TBT-induced elevation in malondialdehyde (MDA) levels in HepG2 cells in a dose-dependent manner. Pretreatment with TSCH increased glutathione reductase (GR) and superoxide dismutase (SOD) activity. Moreover, TSCH decreased the expression of the proapoptotic protein Bax, reducing apoptosis. These results suggest that the protective mechanism of TSCH may be associated with its ability to scavenge MDA, increase antioxidant enzyme activity and downregulate the expression of Bax.

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Funding

This work was supported by the Ocean Public Welfare Scientific Research Special Appropriation Project (201405017), the Major State Basic Research Development Program of China (Grant Nos. 2017YFA0205201), and the Natural Science Foundation of Fujian Province of China (No. 2018 J01067).

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Author notes

  1. Jinpeng Ruan and Junde Chen contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, 361102, Fujian, China

    Jinpeng Ruan, Jie Zeng, Zhenggang Yang, Chonggang Wang & Zhenghong Zuo

  2. Engineering Research Center of Marine Biological Resource Comprehensive Utilization, Third Institute of Oceanography, State Oceanic Administration, 184 Daxue Road, Xiamen, 361005, Fujian, China

    Junde Chen & Zhuan Hong

  3. Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, Fujian, China

    Junde Chen & Zhuan Hong

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  1. Jinpeng Ruan
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Correspondence to Zhuan Hong or Zhenghong Zuo.

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Responsible editor: Philippe Garrigues

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Ruan, J., Chen, J., Zeng, J. et al. The protective effects of Nile tilapia (Oreochromis niloticus) scale collagen hydrolysate against oxidative stress induced by tributyltin in HepG2 cells. Environ Sci Pollut Res 26, 3612–3620 (2019). https://doi.org/10.1007/s11356-018-3729-9

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  • DOI: https://doi.org/10.1007/s11356-018-3729-9

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