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miR-21 is involved in skeletal deficiencies of zebrafish embryos exposed to polychlorinated biphenyls

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Abstract

Polychlorinated biphenyl (PCB) exposure increases the incidence and severity of skeletal diseases, but little is known about the mechanisms that mediate this relationship. We exposed zebrafish embryos to PCB1254 and assessed the changes in bone morphology protein receptor II (BMPRII), which is involved in bone formation and embryonic development, miRNA-21, for which BMPRII is a known target, and calcium metabolism. PCB1254 upregulated the expression of miR-21 and suppressed BMPRII expression. The inhibition of miR-21 reversed the downregulation of BMPRII and alleviated the PCB1254-induced loss of calcium. These findings suggest new biomarkers of developmental defects of the skeleton caused by PCBs.

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Acknowledgments

This study was jointly supported by the Science and Technology Development Funds of Nanjing Medical University (2012NJMU061 & 2013NJMU095).

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Authors and Affiliations

  1. Department of Pediatric Orthopaedics, Children’s Hospital of Nanjing Medical University, 72 Guang Zhou Road, Nanjing, 210008, People’s Republic of China

    Li Ju, Zhiwen Zhou, Bo Jiang, Yue Lou & Zhiqun Zhang

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  1. Li Ju
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  2. Zhiwen Zhou
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  3. Bo Jiang
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  4. Yue Lou
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  5. Zhiqun Zhang
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Correspondence to Zhiqun Zhang.

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All animal care and experimental procedures were approved by the Institutional Animal Care and Use Committee, Nanjing Medical University.

Funding

This study was funded by the Science and Technology Development Funds of Nanjing Medical University (2012NJMU061 & 2013NJMU095).

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The authors declare that they have no conflict of interest.

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Responsible editor: Henner Hollert

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Ju, L., Zhou, Z., Jiang, B. et al. miR-21 is involved in skeletal deficiencies of zebrafish embryos exposed to polychlorinated biphenyls. Environ Sci Pollut Res 24, 886–891 (2017). https://doi.org/10.1007/s11356-016-7874-8

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  • DOI: https://doi.org/10.1007/s11356-016-7874-8

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