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The combined effect of high-intensity interval training and intermittent fasting on lipid profile and peroxidation in Wistar rats under high-fat diet

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Abstract

Purpose

High-fat diet is considered as the main risk factor for the development of dyslipidemia and lipid peroxidation, which in turn results in serious health problems such as cardiovascular disease, type 2 diabetes, and some forms of cancers. Therefore, the purpose of this study was to investigate the combined effect of high-intensity interval training (HIIT) and intermittent fasting (IF) on lipid profile and peroxidation in Wistar rats under a high-fat diet.

Methods

A total of 28 male Wistar rats went through a high-fat diet for 12 weeks. Then, they were randomly divided into four groups: (1) HIIT (3 days week−1 for 6 weeks), (2) IF (3 days week−1 for 6 weeks), (3) combined HIIT and IF (received both treatment for 6 weeks), and (4) control (CON). All groups were under a high-fat diet until the end of the study.

Results

According to the results, LDL levels significantly decreased in the HIIT group compared to the CON group, and in the IF and HIIT + IF groups, a non-significant increase in HDL was observed compared to the CON group. In HIIT + IF and HIIT groups, LDL/HDL ratio decreased significantly compared to the CON group (P < 0.05). Moreover, in IF group, a significant decrease in TG occurred in comparison with HIIT and HIIT + IF groups. A significant increase in serum MDA levels was observed in IF and HIIT + IF groups but not in the HIIT group in comparison with the CON group.

Conclusion

Our results suggest that in subjects under a high-fat diet, both HIIT and IF may help to improve lipid profile, but their combination may not have any synergistic effect. Also, IF and HIIT + IF may increase lipid peroxidation in subjects under a high-fat diet.

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Abbreviations

HFD:

High-fat diet

HIIT:

High-intensity interval training

IF:

Intermittent fasting

LPO:

Lipid peroxidation

MDA:

Malondialdehyde

CVD:

Cardiovascular disease

LDL:

Low-density lipoprotein

HDL:

High-density lipoprotein

TG:

Triglycerides

TC:

Total cholesterol

ROS:

Reactive oxygen species

DNA:

Deoxyribonucleic acid

PUFAs:

Polyunsaturated fatty acids

DR:

Dietary restriction

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Authors and Affiliations

  1. MSc in Exercise Physiology, Shahed University, Tehran, Iran

    Bahman Abbasi

  2. Physical Education and Sport Sciences Department, Faculty of Humanities, Shahed University, Tehran, Iran

    Ali Samadi

  3. Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Behzad Bazgir

Authors
  1. Bahman Abbasi
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  2. Ali Samadi
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  3. Behzad Bazgir
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Correspondence to Ali Samadi.

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All steps of the study were performed in accordance with “Guiding Principles for the Care and Use of Research Animals” approved by the Ethical Committee of Baqiyatallah University of Medical Sciences with a code number of IR.BMSU.REC.1398.022.

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Abbasi, B., Samadi, A. & Bazgir, B. The combined effect of high-intensity interval training and intermittent fasting on lipid profile and peroxidation in Wistar rats under high-fat diet. Sport Sci Health 16, 645–652 (2020). https://doi.org/10.1007/s11332-020-00637-3

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