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Do sex-related differences and time of intervals affect the skeletal muscle glycolytic response to high-intensity interval exercise?‏

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Abstract

Purpose

It may be considered that the duration of intervals of high-intensity interval exercise (HIIE) can affect skeletal muscle biochemical and physiological responses. Also, sex-based differences in muscle biochemistry and physiology play a role in different responses to exercise. The aim of this study was to investigate the effects of sex differences on biochemical parameters involved in muscle glycolysis in response to HIIE protocols with short and long-term intervals.

Methods

Forty male and female Wistar rats (aged 8 weeks, mean weighting 270 g and 225 g, respectively) were divided into HIIE with short-term interval (HIIESh), HIIE with long-term interval (HIIEL), and control groups. The phosphofructokinase (PFK), glycogen synthase 1 (GYS1), monocarboxylate transporter 4 (MCT4), and lactate dehydrogenase (LDH) levels were evaluated in gastrocnemius muscle after a single bout of exercise.

Results

The PFK, GYS1, and MCT4 levels were lower in HIIESh and HIIEL groups compared to the control group. However, there was no significant difference between sexes (P < 0.05). Moreover, the LDH activity was higher in both male and female HIIE rats compared to the control group.

Conclusion

It is likely that HIIE with short- and long-term intervals represents similar effects on the glycolytic pathway in both male and female rats.

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Acknowledgments

We would like to thank Dr. Mehdi Hedayati, the Head of Cellular and Molecular Endocrine Research Center at Shahid Beheshti University of Medical Sciences for his sincere cooperation in conducting laboratory tests.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Exercise Physiology, Faculty of Sport Sciences, University of Birjand, Birjand, Iran

    Marziyeh Saghebjoo, Iman Saffari, Saber Sadeghi-Tabas & Fereshteh Ahmadabadi

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  1. Marziyeh Saghebjoo
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  2. Iman Saffari
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  3. Saber Sadeghi-Tabas
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  4. Fereshteh Ahmadabadi
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Correspondence to Marziyeh Saghebjoo.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures were approved by the Ethics Committee of the Birjand University of Medical Sciences (IR.BUMS.REC.1396.55).

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Saghebjoo, M., Saffari, I., Sadeghi-Tabas, S. et al. Do sex-related differences and time of intervals affect the skeletal muscle glycolytic response to high-intensity interval exercise?‏. Sport Sci Health 16, 473–478 (2020). https://doi.org/10.1007/s11332-020-00627-5

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