Abstract
Introduction
Myasthenia gravis (MG) and rheumatoid arthritis (RA) are examples of antibody-mediated chronic, progressive autoimmune diseases. Phenotypically dissimilar, MG and RA share common immunological features. However, the immunometabolomic features common to humoral autoimmune diseases remain largely unexplored.
Objectives
The aim of this study was to reveal and illustrate the metabolomic profile overlap found between these two diseases and describe the immunometabolomic significance.
Methods
Metabolic analyses using acid- and dansyl-labelled was performed on serum from adult patients with seropositive MG (n = 46), RA (n = 23) and healthy controls (n = 49) presenting to the University of Alberta Hospital specialty clinics. Chemical isotope labelling liquid chromatography mass spectrometry (CIL LC–MS) methods were utilized to assess the serum metabolome in patients; 12C/13C-dansyl chloride (DnsCl) was used to label amine/phenol metabolites and 12C/13C-p-dimethylaminophenacyl bromide (DmPA) was used for carboxylic acids. Metabolites matching our criteria for significance were selected if they were present in both groups. Multivariate statistical analysis [including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA)] and biochemical pathway analysis was then conducted to gain understanding of the principal pathways involved in antibody-mediated pathogenesis.
Results
We found 20 metabolites dysregulated in both MG and RA when compared to healthy controls. Most prominently, observed changes were related to pathways associated with phenylalanine metabolism, tyrosine metabolism, ubiquinone and other terpenoid-quinone biosynthesis, and pyruvate metabolism.
Conclusion
From these results it is evident that many metabolites are common to humoral disease and exhibit significant immunometabolomic properties. This observation may lead to an enhanced understanding of the metabolic underpinnings common to antibody-mediated autoimmune disease. Further, contextualizing these findings within a larger clinical and systems biology context could provide new insights into the pathogenesis and management of these diseases.
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D.B. developed the concept and designed the experiment, acquired all blood samples but those from rheumatoid arthritis patients, conducted all databasing, bioinformatics analyses and metabolite-database matching. Further, D.B. prepared all charts, images and tables and wrote the paper. L.L. developed the chemical labelling process and several chemometric analysis tools used in this study as well as the chemical identification libraries used in the positive identification of observed metabolites. L.L. provided oversight in the choice of chemical and statistical techniques used to characterize the metabolome. L.L also offered conceptual advice, supervised project analysis and edited the manuscript. N.W. performed the sample preparation, chemical labelling and mass spectrometric analysis. W.M provided the rheumatoid arthritis samples for the disease control cohort. E.Y. contributed to study planning and design, procurement of rheumatoid samples and providing periodic guidance. Z.S. developed the concept and provided project oversight as the clinical expert in myasthenia gravis and enrolled study patients. Z.S. also offered conceptual advice, supervised project analysis and edited the manuscript. All authors reviewed the manuscript.
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This work received ethics approval from the University of Alberta Research Ethics Board and is in compliance with the ethical standards of this institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Informed consent was obtained from all individual participants included in the study.
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Blackmore, D., Li, L., Wang, N. et al. Metabolomic profile overlap in prototypical autoimmune humoral disease: a comparison of myasthenia gravis and rheumatoid arthritis. Metabolomics 16, 10 (2020). https://doi.org/10.1007/s11306-019-1625-z
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DOI: https://doi.org/10.1007/s11306-019-1625-z