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Antimicrobial and antibiofilm properties of selenium-chitosan-loaded salicylic acid nanoparticles for the removal of emerging contaminants from bacterial pathogens

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Abstract

In this study salicylic acid loaded containing selenium nanoparticles was synthesized and called SA@CS-Se NPs. the chitosan was used as a natural stabilizer during the synthesis process. Fourier transforms infrared spectroscopy (FTIR), Powder X-ray diffraction (XRD), field emission electron microscopy (FESEM), and transmission electron microscopy (TEM) were used to describe the physicochemical characteristics of the SA@CS-Se NPs. The PXRD examination revealed that the grain size was around 31.9 nm. TEM and FESEM techniques showed the spherical shape of SA@CS-Se NPs. Additionally, the analysis of experiments showed that SA@CS-Se NPs have antibacterial properties against 4 ATCC bacteria; So that with concentrations of 75, 125, 150, and 100 µg/ml, it inhibited the biofilm formation of Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus respectively. Also, at the concentration of 300 µg/ml, it removed 22.76, 23.2, 10.62, and 18.08% biofilm caused by E. coli, P. aeruginosa, B. subtilis, and S. aureus respectively. The synthesized SA@CS-Se NPs may find an application to reduce the unsafe influence of pathogenic microbes and, hence, eliminate microbial contamination.

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Data availability

The datasets generated during and/or analyzed during the current work are available from the corresponding author on reasonable request. Data will be made available on request.

Abbreviations

DDSs:

drug delivery system

SA:

salicylic acid

CS:

chitosan

XRD:

X-ray diffraction

FESEM:

field emission electron microscopy

TEM:

transmission electron microscopy

SA@CS-Se NPs:

selenium-chitosan-loaded salicylic acid nanoparticles

MIC:

minimum growth inhibitory concentration

MBC:

minimum bactericidal concentration

TSB:

tryptone soy broth

FTIR:

fourier transforms infrared spectroscopy

OD:

optical density

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Authors and Affiliations

  1. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

    Mahla Sadat Bagherian, Parisa Zargham, Hoda Zarharan, Maleknaz Bakhtiari, Negin Mortezaee Ghariyeh Ali & Ali Es-haghi

  2. Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

    Ehsan Yousefi

  3. Department of Pharmacology, Medicinal Plants Pharmacological Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Ehsan Taghavizadeh Yazdi

  4. Applied Biomedical Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Ehsan Taghavizadeh Yazdi

Authors
  1. Mahla Sadat Bagherian
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  2. Parisa Zargham
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  3. Hoda Zarharan
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  7. Ali Es-haghi
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  8. Mohammad Ehsan Taghavizadeh Yazdi
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Contributions

MSB, PZ, HZ, MB, NMGA, and EY: Conceptualization, Methodology, Software; EY, AE, and METY: Data curation, Writing- Original draft preparation; MSA, PZ, HZ, MB, NMGA: Visualization, Investigation; AE, METY: Supervision, Validation, Writing- Reviewing and Editing; All authors discussed the results and contributed to the final manuscript.

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Correspondence to Ali Es-haghi or Mohammad Ehsan Taghavizadeh Yazdi.

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This investigation conformed to the Islamic Azad University and it was approved by the Research Ethics Committee of Islamic Azad University. Consent to participate/consent to participate statements are not applicable.

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Bagherian, M.S., Zargham, P., Zarharan, H. et al. Antimicrobial and antibiofilm properties of selenium-chitosan-loaded salicylic acid nanoparticles for the removal of emerging contaminants from bacterial pathogens. World J Microbiol Biotechnol 40, 86 (2024). https://doi.org/10.1007/s11274-024-03917-z

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