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Levilactobacillus brevis MZ384011 and  Levilactobacillus brevis MW362779 can mitigate lead induced hepato-renal damage by regulating visceral dispersion and fecal excretion

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Abstract

Heavy metal pollution is a global issue. Current study provides evidence on Pb toxicity ameliorative potential and safe nature of Levilactobacillus brevis MZ384011 (S1) and Levilactobacillus brevis MW362779 (S2), isolated from carnivore gut and human milk, respectively. In a 60-days experiment, the rats were distributed into six groups. G-I, G-V and G-VI were kept on normal diet, while GII-IV were fed on lead nitrate (500 mg/kg) supplemented food, throughout experiment. After confirmation of Pb toxicity in GII-IV at 15th day, S1 was orally administered to G-III and G-V while S2 was given to G-IV and G-VI at a dose of 1 × 109 CFU/animal/day. On day 60 of experiment, positive control (G-II) displayed significant reduction in body weight, total protein, albumin, globulin, mineral profile, erythrocyte count, hemoglobin, hematocrit and hematological indices and elevation in leukocyte count, alanine aminotransferase, aspartate aminotransferase, bilirubin, uric acid and creatinine along with alterations in hepato-renal architecture. With reference to G-II, the G-III and G-IV displayed significant improvement in all aforementioned parameters, 40–60% reduction in tissue Pb levels (blood, liver, kidney and adipose tissue) and elevation in fecal Pb contents (p = 0.000). The groups V and VI did not show any sign of toxicity. The findings confirm that strains are safe for biological application and can reverse Pb toxicity by facilitating fecal Pb excretion and reducing its systemic dispersal. To best of our information this is the first report on Pb toxicity ameliorative role of Levilactobacillus brevis from human milk, the safest source.

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Acknowledgements

The authors are thankful to the authorities of Institute of Zoology and Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan, for facilitating with required resources to complete this study.

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Authors and Affiliations

  1. Institute of Zoology, University of the Punjab, Lahore, 54590, Pakistan

    Maria Mushtaq, Najma Arshad, Ghulam Ayesha Javed, Aneela Munir, Mamoona Hameed & Saman Javed

  2. Department of Zoology, Institute of Molecular Biology and Biotechnology (IMBB), Centre for Research in Molecular Medicine (CRIMM), The University of Lahore, Lahore, 54792, Pakistan

    Najma Arshad

  3. Institute of Microbiology and Molecular Genetics (MMG), University of the Punjab, Lahore, 54590, Pakistan

    Abdul Rehman

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  1. Maria Mushtaq
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Contributions

Maria Mushtaq: Engendering idea, investigation, acquisition of data, conceptualization, analysis and writing original manuscript. Najma Arshad: Data analysis and editing, supervision, validation of written manuscript and project administration. Abdul Rehman: Validation, formal analysis and visualization. Ghulam Ayesha Javed: Isolation and characterization of the strain Levilactobacillus brevis MZ384011. Aneela Munir: Isolation and characterization of the strain Levilactobacillus brevis MW362779. Mamoona Hameed: Interpretation of data. Saman Javed: Formal analysis and visualization.

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Correspondence to Najma Arshad.

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The Advance Board of Studies and Ethical Committee for Animal Usage, Institute of Zoology, University of the Punjab, Lahore, Pakistan granted the approval for animal experimentation (039/dfems dated January, 15, 2020) and all the tests were performed according to the guidelines of ARRIVE (Animal Research: Reporting of In Vivo Experiments) and ICLAS (International Council for Laboratory Animal Science).

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Mushtaq, M., Arshad, N., Rehman, A. et al. Levilactobacillus brevis MZ384011 and  Levilactobacillus brevis MW362779 can mitigate lead induced hepato-renal damage by regulating visceral dispersion and fecal excretion. World J Microbiol Biotechnol 40, 74 (2024). https://doi.org/10.1007/s11274-023-03818-7

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