Abstract
Since ancient time, plants and there parts have been used widely against dreadful pathogens due to ability of killing microbes. Waste from fruits and vegetables are pulling in more interest in exploration due to their therapeutic properties such as anti-pathogenic activity. In the present study antimicrobial and cytotoxicity properties of herbal combination prepared from peels of Allium cepa, Cucumis sativus, Citrus reticulata, and Mangifera indica were investigated. The herbal combination was tested for broad spectrum antimicrobial activity against Streptococcus mutans, Bacillus licheniformis, Lactobacillus plantarum, Escherichia coli, Pseudomonas aeruginosa, and Citrobacter freundii. The presence of phytochemical markers such as phenolics and flavanoids were also investigated. The results revealed that the herbal combination exhibited antimicrobial activity against S. mutans, B. licheniformis, L. plantarum, E. coli and moderate against P. aeruginosa and C. freundii. The presence of phenolics (798 ± 1.52 µg/g) and flavanoids (355.3 ± 2.081 µg/g) was also detected. Also, the herbal combination contains flavanoids such as quercetin and rutin was confirmed with the help of column chromatography, high-performance liquid chromatography (HPLC), fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR) analysis. The herbal combination helps in the proliferation of L929 cells without affecting their viability was confirmed by MTT assay. The results of the present research suggests the possibility to use herbal combination as source for plausible antibacterial agent which could be isolated and used as a lead candidate for the development of antibacterial drug that help to limit or stop infectious illnesses caused by different pathogenic microbes.
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Acknowledgements
The authors are thankful to Prof. Ina Aditya Shastri Vice-Chancellor, Banasthali Vidyapith and Prof. Dipjyoti Chakraborty, Head of the Department, Bioscience and Biotechnology, Banasthali Vidyapith for their encouragement and providing scientific platform to carry out present work. We are also thankful to DBT supported Bioinformatics Center, for providing computational assistance and Dr Swati Paliwal, Associate professor, Department of Bioscience and Biotechnology, Banasthali Vidyapith, for providing guidance during cell culture work.
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JM and NS conceived the present idea of writing the research and designed the content. JM and NS encouraged to investigate and supervised the findings of this work. AS developed the theory and performed the experiments. AS contributed to the analysis of the results and writing of the manuscript.
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Anjani, Mathur, J. & Srivastava, N. Value added bioactive compounds from fruits & vegetables waste for assessing their antimicrobial activity. World J Microbiol Biotechnol 39, 71 (2023). https://doi.org/10.1007/s11274-022-03488-x
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DOI: https://doi.org/10.1007/s11274-022-03488-x