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Saccharomyces cerevisiae strains associated with the production of cachaça: identification and characterization by traditional and molecular methods (PCR, PFGE and mtDNA-RFLP)

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Abstract

A study of the genetic diversity of populations of Saccharomyces cerevisiae was conducted in ten different cachaça producers (alambiques) in the southern state of Minas Gerais, Brazil. A total of 106 isolates were identified by PCR using the primer SCREC114, specific to S. cerevisiae, by pulsed-field gel electrophoresis (PFGE) and by restriction fragment polymorphism of mitochondrial DNA analysis (RFLP-mtDNA). PCR showed a product of amplification to 61 isolates, enabling a rapid identification of S. cerevisiae in different alambiques. Nine different profiles were found by PFGE; all the yeasts identified as S. cerevisiae by PCR had profiles similar to that of the marker S. cerevisiae, highlighting the specificity of primer SCREC114. RFLP-mtDNA, using four different enzymes, enabled the grouping of strains of S. cerevisiae, with 80%–100% similarity. Some alambiques that had a higher frequency of S. cerevisiae characterized by PCR and PFGE, had a lower level of genetic diversity determined by RFLP-mtDNA, indicating the ability of these strains to lead the fermentative process.

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Acknowledgments

The authors thank Fundação de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig) for financial support. TLB and GVMP thank Conselho Nacional de Desenvolvimento Científico e Tecnológico do Brasil (CNPq) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for scholarships.

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Authors and Affiliations

  1. Biology Department, Federal University of Lavras, 37.200-000, Lavras, MG, Brazil

    Taís Letícia Bernardi, Gilberto Vinícius de Melo Pereira, Patricia Gomes Cardoso, Eustáquio Souza Dias & R. F. Schwan

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  1. Taís Letícia Bernardi
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  2. Gilberto Vinícius de Melo Pereira
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  3. Patricia Gomes Cardoso
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  4. Eustáquio Souza Dias
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  5. R. F. Schwan
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Correspondence to R. F. Schwan.

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Bernardi, T.L., de Melo Pereira, G.V., Cardoso, P.G. et al. Saccharomyces cerevisiae strains associated with the production of cachaça: identification and characterization by traditional and molecular methods (PCR, PFGE and mtDNA-RFLP). World J Microbiol Biotechnol 24, 2705–2712 (2008). https://doi.org/10.1007/s11274-008-9799-y

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