Abstract
In the present study, turkey proventriculus (stomach) was mixed with 10% NaCl (1:2 w/v) and extracted by centrifugation to produce crude extract. The partial purification of the extract was carried out by using Sephadex G-75 resin in gel filtration column chromatography. Crude extract (CE) and fractions obtained by gel filtration were analysed for milk-clotting activity (MCA), protein content, proteolytic activity (PA), and SDS-PAGE electrophoresis. The first 7 fractions did not have any activities; the fraction of 9, 10 and 11 had the most milk-clotting and proteolytic activities. Electrophoretic patterns proved that further purification steps should be applied for better results.
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The authors thank Ondokuz Mayis University Research Fund to support this project.
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Temiz, H., Okumus, E., Aykut, U. et al. Partial purification of pepsin from turkey proventriculus. World J Microbiol Biotechnol 24, 1851–1855 (2008). https://doi.org/10.1007/s11274-008-9678-6
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DOI: https://doi.org/10.1007/s11274-008-9678-6