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3H-117, a structural protein of Heliothis virescens ascovirus 3h (HvAV-3h)

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Abstract

The open reading frame 117 (3h-117) of Heliothis virescens ascovirus 3h (HvAV-3h), which is a conserved coding region present in all completely sequenced ascovirus members, was characterized in this study. By RT-PCR detection, 3h-117 transcription began at 6-h post-infection (hpi) and remained stable until 168 hpi in HvAV-3h-infected Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) larvae. In addition, 3h-117 putatively encodes a 21.5-kDa protein (3H-117) predicted to be a CTD-like phosphatase. Western blot analysis using a prepared rabbit polyclonal antibody specific to 3H-117 showed that the product could be detected at 24 hpi, which remained stably detectable until 168 hpi. The same analysis also demonstrated that the 3H-117 protein localized in the virions of HvAV-3h. Immunofluorescence analysis showed that at 24 hpi, 3H-117 was mainly located in the nuclei of H. armigera larval fat body cells and later spread into the cytoplasm. In summary, our results indicate that 3H-117 is a structural protein of HvAV-3h.

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Acknowledgements

The authors would like to thank Mrs. Youlin Zhu (Wuhan Institute of Virology, Wuhan, China) for her help during antibody preparation and Dr. Cecil L. Smith (University of Georgia, USA) for editing the English language. This study was supported partly by the National Natural Science Foundation of China (31872027).

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Authors and Affiliations

  1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Changsha, 410128, China

    Ying Zhao, Huan Yu, Lei He, Ni Li & Guo-Hua Huang

  2. College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China

    Ying Zhao, Huan Yu, Lei He, Ni Li & Guo-Hua Huang

Authors
  1. Ying Zhao
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  2. Huan Yu
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  3. Lei He
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  4. Ni Li
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  5. Guo-Hua Huang
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Contributions

YZ, HY, and GHH conceived and designed the experiments. YZ, NL, and LH performed the experiments. YZ, GHH wrote the manuscript. All the authors discussed and commented on the manuscript.

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Correspondence to Guo-Hua Huang.

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Edited by Seung-Kook Choi.

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Zhao, Y., Yu, H., He, L. et al. 3H-117, a structural protein of Heliothis virescens ascovirus 3h (HvAV-3h). Virus Genes 55, 688–695 (2019). https://doi.org/10.1007/s11262-019-01679-7

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  • DOI: https://doi.org/10.1007/s11262-019-01679-7

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