Skip to main content
SpringerLink
Log in

Characterization of H5N1 influenza A viruses isolated from domestic green-winged teal

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Two avian influenza virus strains, A/domestic green-winged teal/Hunan/67/2005 (H5N1) (D-GWT/67) and A/domestic green-winged teal/Hunan/79/2005 (H5N1) (D-GWT/79), were isolated from healthy domestic green-winged teals (Anas crecca) in Hunan Province, South China. Genomic analysis showed that both isolates were reassortants. The hemagglutinin (HA) genes of the two isolates were closely related to that of an H5N1 strain isolated from tree sparrow (A/tree sparrow/Henan/1/04). The neuraminidase (NA) genes and the internal protein genes of both isolates were closely related to those from A/chicken/Shantou/4231/2003-like (H5N1) viruses, with exception of the matrix (M) gene of D-GWT/79, which was closely related to that of the H7N3 strain A/mallard/Netherlands/12/2000 isolated from wild mallard duck. The virulence of the two isolates was examined in chickens, ducks, and mice. Both strains were found to be highly pathogenic in chickens and ducks, but showed low pathogenicity in mice. These findings contribute to the realization that domestic green-winged teals carrying the H5N1 virus may play an important role in transmitting the virus among birds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. M. Mase, K. Tsukamoto, T. Imada, K. Imai, N. Tanimura, K. Nakamura et al., Virology 332, 167–176 (2005). doi:https://doi.org/10.1016/j.virol.2004.11.016

    Article  CAS  Google Scholar 

  2. C.W. Lee, D.L. Suarez, T.M. Tumpey, H.W. Sung, Y.K. Kwon, Y.J. Lee et al., J. Virol. 79, 3692–3702 (2005). doi:https://doi.org/10.1128/JVI.79.6.3692-3702.2005

    Article  CAS  PubMed Central  Google Scholar 

  3. World Health Organization Global Influenza Program Surveillance Network, Emerg. Infect. Dis. 11, 1515–1521 (2005)

    Article  Google Scholar 

  4. World Health Organization, http://www.who.int/csr/disease/avian_influenza/country/cases_table_2007_10_08/en/index.html (2007)

  5. World Health Organization, http://www.who.int/csr/disease/avian_influenza/timeline_10_09_2007.pdf (2006)

  6. X. Xu, K. Subbarao, N.J. Cox, Y. Guo, Virology 261, 15–19 (1999). doi:https://doi.org/10.1006/viro.1999.9820

    Article  CAS  Google Scholar 

  7. Y. Guan, K.F. Shortridge, S. Krauss, R.G. Webster, Proc. Natl. Acad. Sci. USA 96, 9363–9367 (1999). doi:https://doi.org/10.1073/pnas.96.16.9363

    Article  CAS  Google Scholar 

  8. E. Hoffmann, J. Stech, I. Leneva, S. Krauss, C. Scholtissek, P.S. Chin et al., J. Virol. 74, 6309–6315 (2000). doi:https://doi.org/10.1128/JVI.74.14.6309-6315.2000

    Article  CAS  PubMed Central  Google Scholar 

  9. K.S. Li, Y. Guan, J. Wang, G.J. Smith, K.M. Xu, L. Duan et al., Nature 430, 209–213 (2004). doi:https://doi.org/10.1038/nature02746

    Article  CAS  Google Scholar 

  10. L.J. Reed, H. Muench, Am. J. Hyg. 27, 493–497 (1938)

    Google Scholar 

  11. S. Kumar, K. Tamura, M. Nei, Brief. Bioinform. 5, 150–163 (2004). doi:https://doi.org/10.1093/bib/5.2.150

    Article  CAS  Google Scholar 

  12. D.A. Senne, B. Panigrahy, Y. Kawaoka, J.E. Pearson, J. Suss, M. Lipkind et al., Avian Dis. 40, 425–437 (1996). doi:https://doi.org/10.2307/1592241

    Article  CAS  Google Scholar 

  13. J. Stevens, O. Blixt, T.M. Tumpey, J.K. Taubenberger, J.C. Paulson, I.A. Wilson, Science 312, 404–410 (2006). doi:https://doi.org/10.1126/science.1124513

    Article  CAS  Google Scholar 

  14. M.D. de Jong, T.T. Thanh, T.H. Khanh, V.M. Hien, G.J.D. Smith, N.V. Chau, B.V. Cam, P.T. Qui, D.Q. Ha, Y. Guan, J.S. Malik Peiris, T.T. Hien, J. Farrar, N. Engl. J. Med. 353, 2667–2672 (2005). doi:https://doi.org/10.1056/NEJMoa054512

    Article  Google Scholar 

  15. M. Hatta, P. Gao, P. Halfmann, Y. Kawaoka, Science 293, 1840–1842 (2001). doi:https://doi.org/10.1126/science.1062882

    Article  CAS  Google Scholar 

  16. S.H. Seo, E. Hoffmann, R.G. Webster, Nat. Med. 8, 950–954 (2002). doi:https://doi.org/10.1038/nm757

    Article  CAS  Google Scholar 

  17. A.S. Lipatov, S. Andreansky, R.J. Webby, D.J. Hulse, J.E. Rehg, S. Krauss et al., J. Gen. Virol. 86, 1121–1130 (2005). doi:https://doi.org/10.1099/vir.0.80663-0

    Article  CAS  Google Scholar 

  18. A.J. Hay, A.J. Wolstenholme, J.J. Skehel, M.H. Smith, EMBO J. 4, 3021–3024 (1985)

    Article  CAS  PubMed Central  Google Scholar 

  19. L.H. Pinto, L.J. Holsinger, R.A. Lamb, Cell 69, 517–528 (1992). doi:https://doi.org/10.1016/0092-8674(92)90452-I

    Article  CAS  Google Scholar 

  20. M. Mase, J.H. Kim, Y.J. Lee, K. Tsukamoto, T. Imada, K. Imai et al., Microbiol. Immunol. 49, 871–874 (2005)

    Article  CAS  Google Scholar 

  21. A.J. Buckler-White, C.W. Naeve, B.R. Murphy, J. Virol. 57, 697–700 (1986)

    CAS  PubMed  PubMed Central  Google Scholar 

  22. G. Neumann, Y. Kawaoka, Emerg. Infect. Dis. 12, 881–886 (2006)

    Article  PubMed Central  Google Scholar 

  23. J.Y. Zhou, H.G. Shen, H.X. Chen, G.Z. Tong, M. Liao, H.C. Yang et al., J. Gen. Virol. 87, 1823–1833 (2006). doi:https://doi.org/10.1099/vir.0.81800-0

    Article  CAS  Google Scholar 

  24. K.M. Sturm-Ramirez, T. Ellis, B. Bousfield, L. Bissett, K. Dyrting, J.E. Rehg et al., J. Virol. 78, 4892–4901 (2004). doi:https://doi.org/10.1128/JVI.78.9.4892-4901.2004

    Article  CAS  PubMed Central  Google Scholar 

  25. J.M. Katz, X. Lu, T.M. Tumpey, C.B. Smith, M.W. Shaw, K. Subbarao, J. Virol. 74, 10807–10810 (2000). doi:https://doi.org/10.1128/JVI.74.22.10807-10810.2000

    Article  CAS  PubMed Central  Google Scholar 

  26. J. Liu, H. Xiao, F. Lei, Q. Zhu, K. Qin, X.W. Zhang et al., Science 309, 1206 (2005). doi:https://doi.org/10.1126/science.1115273

    Article  CAS  Google Scholar 

  27. V.J. Munster, C. Baas, P. Lexmond, J. Waldenstrom, A. Wallensten, T. Fransson, et al., PLoS Pathog. (2007). doi:https://doi.org/10.1371/journal.ppat.0030061

    Article  PubMed Central  Google Scholar 

  28. B. Olsen, V.J. Munster, A. Wallensten, J. Waldenstrom, A.D. Osterhaus, R.A. Fouchier, Science 312, 384–388 (2006). doi:https://doi.org/10.1126/science.1122438

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the research funds from European Commission 6th Framework Program on Research, Technological Development and Demonstration (FP6-2005-SSP-5-B-INFLUENZA), National Key Technology R&D Program of China (2006BAD06A03), National 973 Project (2005CB523007, 2006CB0N1202), Chinese Academy of Sciences (KSCX1-YW-R-14), and Hunan Provincial Science and Technology Department (2006NK2003).

Author information

Authors and Affiliations

  1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China

    Jianjun Chen, Quanjiao Chen & Ze Chen

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Jianjun Chen

  3. Shanghai Institute of Biological Products, Shanghai, 200052, China

    Zhongdong Yang, Xueying Liu, Dongmei Li & Ze Chen

  4. College of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, China

    Fang Fang, Haiyan Chang & Ze Chen

Authors
  1. Jianjun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhongdong Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Quanjiao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xueying Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fang Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Haiyan Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dongmei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ze Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ze Chen.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, J., Yang, Z., Chen, Q. et al. Characterization of H5N1 influenza A viruses isolated from domestic green-winged teal. Virus Genes 38, 66–73 (2009). https://doi.org/10.1007/s11262-008-0289-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-008-0289-y

Keywords

Search

Navigation