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Molecular characterization and function of JAK/STAT pathway in IPEC-J2 cells during Clostridium perfringens beta2 toxin stimulation

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Abstract

Intestinal infection with C. perfringens is responsible for outbreaks of diarrhea in piglets. Janus kinase / signal transducer and activator of transcription (JAK/STAT) is a vital signaling pathway that regulates cellular activity and inflammatory response, closely correlated with multiple diseases development and advances. Currently, the potential effect of JAK/STAT on C. perfringens beta2 (CPB2) treatment on porcine intestinal epithelial (IPEC-J2) cells has not been explored. The expression of JAK/STAT genes or proteins in IPEC-J2 cells induced by CPB2 were observed by qRT-PCR and Western blot, and further used WP1066 to explore the effect of JAK2/STAT3 on mechanism employed by CPB2 on apoptosis, cytotoxicity, oxidative stress and inflammatory cytokines of IPEC-J2 cells. JAK2, JAK3, STAT1, STAT3, STAT5A and STAT6 were highly expressed in CPB2-induced IPEC-J2 cells, among which STAT3 had the highest expression. Moreover, apoptosis, cytotoxicity and oxidative stress were attenuated via blocking the activation of JAK2/STAT3 by using WP1066 in CPB2-treated IPEC-J2 cells. Furthermore, WP1066 significantly suppressed the secretion of interleukin (IL)-6, IL-1β and TNF-α induced by CPB2 in IPEC-J2 cells.Our findings provide some insights into the functional roles of JAK2/STAT3 in piglets against to C. perfringens infection.

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Funding

This research was supported by Scientific Research Start-Up Funds for Openly-Recruited Doctors of Gansu Agricultural University (GAU-KYQD-2021-04).

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Authors and Affiliations

  1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China

    Xiaoli Gao, Pengfei Wang, Zunqiang Yan, Qiaoli Yang, Xiaoyu Huang & Shuangbao Gun

  2. Farmer Education and Training Work Station of Gansu province, Lanzhou, 730070, China

    Shengwei Zhang

  3. Gansu Research Center for Swine Production Engineering and Technology, Lanzhou, 730070, China

    Shuangbao Gun

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  1. Xiaoli Gao
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  2. Pengfei Wang
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  5. Xiaoyu Huang
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  6. Shengwei Zhang
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  7. Shuangbao Gun
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Contributions

XG and SG conceptualized the study. XG performed the experiments. ZY and XH analyzed the data. PW illustrated the figures. SZ and QY performed supervision of the study. XG wrote the original draft. SG reviewed and edited the manuscript.

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Correspondence to Shuangbao Gun.

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Gao, X., Wang, P., Yan, Z. et al. Molecular characterization and function of JAK/STAT pathway in IPEC-J2 cells during Clostridium perfringens beta2 toxin stimulation. Vet Res Commun 47, 1177–1184 (2023). https://doi.org/10.1007/s11259-023-10118-w

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