Abstract
This study aimed to investigate sites for colonization and molecular epidemiology of antimicrobial-resistant Pseudomonas aeruginosa in a veterinary teaching hospital. Bacterial specimens from surface and liquid samples (n = 165) located in five rooms were collected three times every 2 months, and antimicrobial susceptibility was subsequently determined by minimum inhibitory concentrations. The genomes of resistant strains were further analyzed using whole-genome sequencing. Among 19 P. aeruginosa isolates (11.5%, 19/165), sinks were the most frequent colonization site (53.3%), followed by rubber tubes (44.4%), and anesthesia-breathing circuit (33.3%). The highest resistance to gentamicin (47.4%), followed by piperacillin/tazobactam (36.8%), levofloxacin (36.8%), and ciprofloxacin (36.8%), was observed from 19 P. aeruginosa isolates, of which 10 were resistant strains. Of these 10 antimicrobial-resistant isolates, five were multidrug-resistant isolates, including carbapenem. From the multilocus sequence typing (MLST) analysis, five sequence types (STs), including a high-risk clone of human ST235 (n = 3), and ST244 (n = 3), ST606 (n = 2), ST485 (n = 1), and ST3405 (n = 1) were identified in resistant strains. Multiresistant genes were identified consistent with STs, except ST235. The MLST approach and single nucleotide polymorphism analysis revealed a link between resistant strains from ward rooms and those from examination, wound care, and operating rooms. The improvement of routine cleaning, especially of sink environments, and the continued monitoring of antimicrobial resistance of P. aeruginosa in veterinary hospitals are necessary to prevent the spread of resistant clones and ensure infection control.
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Data Availability
All read data were deposited in the NCBI database under the BioProject ID PRJNA748228. This whole genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAHWUT000000000 (strain S11.3), JAHWUU000000000 (strain W1.3), JAHWUV000000000 (strain WA1.3), JAHWUW000000000 (strain O6.2), JAHWUX000000000 (strain WA10.2), JAHWUY000000000 (strain ER10.2), JAHWUZ000000000 (strain ER5.2), JAHWVA000000000 (strain WA1.2), JAHWVB000000000 (strain O1.1), and JAHWVC000000000 (strain WA1.1).
Code availability
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Acknowledgements
We thank the director of Prasu-Arthorn animal hospital, Faculty of Veterinary Science, Mahidol University for supporting the bacterial collection procedures.
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This research project was supported by Faculty of Veterinary Science, Mahidol University.
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Conceptualization: Jeerawat Soonthornsit, Nathita Phumthanakorn; Methodology: Jeerawat Soonthornsit, Nathita Phumthanakorn, Kotchawan Pimwaraluck, Noppakhun Kongmuang, Ploy Pratya; Formal analysis and investigation: Jeerawat Soonthornsit, Nathita Phumthanakorn; Writing—original draft preparation: Jeerawat Soonthornsit, Kotchawan Pimwaraluck, Noppakhun Kongmuang, Ploy Pratya, Nathita Phumthanakorn; Writing—review and editing: Nathita Phumthanakorn; Funding acquisition: Nathita Phumthanakorn.
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Soonthornsit, J., Pimwaraluck, K., Kongmuang, N. et al. Molecular epidemiology of antimicrobial-resistant Pseudomonas aeruginosa in a veterinary teaching hospital environment. Vet Res Commun 47, 73–86 (2023). https://doi.org/10.1007/s11259-022-09929-0
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DOI: https://doi.org/10.1007/s11259-022-09929-0