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Genome-wide identification and characterization of novel long non-coding RNA in Ruminal tissue affected with sub-acute Ruminal acidosis from Holstein cattle

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Abstract

Sub-acute ruminal acidosis is a type of metabolic disorder in which affected cattle show a considerable depression of rumen pH. This leads to a dramatic decline in productivity and consequent loss of income for many dairy farms. The objective of the present study is to identify and characterize novel long non-coding RNAs (lncRNAs) in Holstein cattle affected by sub-acute ruminal acidosis. Two replicates from six animals were sequenced that bioinformatically analyzed. Results showed 6679 novel lncRNAs among which 12 intergenic lncRNAs showed differential expression (p value ≤0.05). GO and KEGG analysis revealed that calcium signaling and G protein couple-receptor pathways may be involved in regulating metabolic processes during sub-acute ruminal acidosis. Furthermore, other biological processes including transmembrane transport, adult behavior, neuroactive ligand-receptor interaction, GABAergic synapse, cholinergic synapse were significantly enriched. The present data suggest that these differentially expressed lncRNAs may play regulatory roles in modulating biological processes associated with sub-acute ruminal acidosis in cattle rumen.

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Acknowledgements

Authors declare that there is no any conflict of interest. Authors would like to acknowledge a support given to this study by Agricultural Sciences and Natural Resources University of Khuzestan.

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Authors and Affiliations

  1. Department of Animal science, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran

    Bizhan Mahmoudi, Jamal Fayazi, Hedayatollah Roshanfekr & Mohsen Sari

  2. Department of Animal and poultry science, College of Aburaihan, University of Tehran, Tehran, Iran

    Mohammad Reza Bakhtiarizadeh

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  1. Bizhan Mahmoudi
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  2. Jamal Fayazi
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  3. Hedayatollah Roshanfekr
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  4. Mohsen Sari
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Correspondence to Jamal Fayazi.

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Mahmoudi, B., Fayazi, J., Roshanfekr, H. et al. Genome-wide identification and characterization of novel long non-coding RNA in Ruminal tissue affected with sub-acute Ruminal acidosis from Holstein cattle. Vet Res Commun 44, 19–27 (2020). https://doi.org/10.1007/s11259-020-09769-w

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