Skip to main content

Advertisement

SpringerLink
Log in

The correlation of anti-β2-glycoprotein I antibody with complement activation in patients with idiopathic membranous nephropathy

  • Nephrology - Original Paper
  • Published:
International Urology and Nephrology Aims and scope Submit manuscript

Abstract

Purpose

This study evaluated the correlation of anti-β2-glycoprotein I (anti-β2-GPI) antibodies with complement activation in patients with idiopathic membranous nephropathy (IMN).

Methods

Thirty-two IMN patients with positive anti-β2-GPI antibody were enrolled, and 32 age- and sex-matched IMN patients with negative anti-β2-GPI antibody were randomly enrolled as controls. The frozen serum samples of these 64 patients were collected for detection of anti-phospholipase A2 receptor (PLA2R) antibody and the activity of three complement pathways. Paraffin specimens of the kidney tissues of these 64 patients were collected for immunohistochemical staining of C4d.

Results

IMN patients with positive anti-β2-GPI antibody had a significant decline of the residual complement activity of alternative pathway than those with negative anti-β2-GPI antibody (37.4 ± 21.2% vs 65.7 ± 50.5%, P = 0.021). The positive rate of kidney C4d staining in IMN patients with and without anti-β2-GPI was 65.6 and 81.2%, with no significant difference (P = 0.257). Patients with and without anti-β2-GPI had the same positive rate of anti-PLA2R antibody.

Conclusion

Anti-β2-GPI antibody was associated with alternative complement activation in patients with IMN.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Couser WG (2017) Primary membranous nephropathy. Clin J Am Soc Nephrol 12:983–997

    Article  CAS  Google Scholar 

  2. Cattran DC, Brenchley PE (2017) Membranous nephropathy: integrating basic science into improved clinical management. Kidney Int 91:566–574

    Article  CAS  Google Scholar 

  3. Beck LH, Bonegio RG, Lambeau G et al (2009) M-type phospholipase A2 receptor as target antigen in idiopathic membranous nephropathy. N Engl J Med 361:11–21

    Article  CAS  Google Scholar 

  4. Tomas NM, Beck LH, Meyer-Schwesinger C et al (2014) Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy. N Engl J Med 371:2277–2287

    Article  Google Scholar 

  5. Sethi S, Debiec H, Madden B et al (2020) Neural epidermal growth factor-like 1 protein (NELL-1) associated membranous nephropathy. Kidney Int 97:163–174

    Article  CAS  Google Scholar 

  6. Pozdzik A, Brochériou I, David C et al (2018) Membranous nephropathy and anti-podocytes antibodies: implications for the diagnostic workup and disease management. Biomed Res Int 2018:6281054

    Article  Google Scholar 

  7. Ma H, Sandor DG, Beck LH Jr (2013) The role of complement in membranous nephropathy. Semin Nephrol 33:531–542

    Article  CAS  Google Scholar 

  8. Francis JM, Beck LH Jr, Salant DJ (2016) Membranous nephropathy: a journey from bench to bedside. Am J Kidney Dis 68:138–147

    Article  CAS  Google Scholar 

  9. Salant DJ (2019) Unmet challenges in membranous nephropathy. Curr Opin Nephrol Hypertens 28:70–76

    Article  Google Scholar 

  10. Sciascia S, Radin M (2018) Thrombotic antiphospholipid syndrome. Lupus 27:21–27

    Article  CAS  Google Scholar 

  11. Levine JS, Branch DW, Rauch J (2002) The antiphospholipid syndrome. N Engl J Med 346:752–763

    Article  CAS  Google Scholar 

  12. Levy Y, Ziporen L, Gilburd B et al (1996) Membranous nephropathy in primary antiphospholipid syndrome: description of a case and induction of renal injury in SCID mice. Hum Antibodies Hybridomas 7:91–96

    Article  CAS  Google Scholar 

  13. Sinico RA, Cavazzana I, Nuzzo M et al (2010) Renal involvement in primary antiphospholipid syndrome: retrospective analysis of 160 patients. Clin J Am Soc Nephrol 5:1211–1217

    Article  CAS  Google Scholar 

  14. Zhang Q, Wang Y, Xu Y et al (2019) Antiphospholipid antibodies in patients with membranous nephropathy. Nephron 143:228–233

    Article  CAS  Google Scholar 

  15. Val-Bernal JF, Garijo MF, Val D et al (2011) C4d immunohistochemical staining is a sensitive method to confirm immunoreactant deposition in formalin-fixed paraffin-embedded tissue in membranous glomerulonephritis. Histol Histopathol 26:1391–1397

    CAS  PubMed  Google Scholar 

  16. Borza DB, Zhang JJ, Beck LH Jr et al (2013) Mouse models of membranous nephropathy: the road less travelled by. Am J Clin Exp Immunol 2:135–145

    PubMed  PubMed Central  Google Scholar 

  17. Jelezarova E, Lutz HU (2005) IgG naturally occurring antibodies stabilize and promote the generation of the alternative complement pathway C3 convertase. Mol Immunol 42:1393–1403

    Article  CAS  Google Scholar 

  18. Luo W, Olaru F, Miner JH et al (2018) Alternative pathway is essential for glomerular complement activation and proteinuria in a mouse model of membranous nephropathy. Front Immunol 9:1433

    Article  Google Scholar 

  19. Pierangeli SS, Vega-Ostertag M, Liu X et al (2005) Complement activation: a novel pathogenic mechanism in the antiphospholipid syndrome. Ann N Y Acad Sci 1051:413–420

    Article  CAS  Google Scholar 

  20. Ruiz-Irastorza G, Crowther M, Branch W et al (2010) Antiphospholipid syndrome. Lancet 376:1498–1509

    Article  CAS  Google Scholar 

  21. Oku K, Atsumi T, Bohgaki M et al (2009) Complement activation in patients with primary antiphospholipid syndrome. Ann Rheum Dis 68:1030–1035

    Article  CAS  Google Scholar 

  22. Zhou Y, Chen P, Li Y (2019) Association between antiphospholipid antibodies and factor Bb in lupus nephritis patients with glomerular microthrombosis. Int J Rheum Dis 22:2185–2190

    Article  CAS  Google Scholar 

  23. Lionaki S, Derebail VK, Hogan SL et al (2012) Venous thromboembolism in patients with membranous nephropathy. Clin J Am Soc Nephrol 7:43–51

    Article  CAS  Google Scholar 

  24. Barbour SJ, Greenwald A, Djurdjev O et al (2012) Disease-specific risk of venous thromboembolic events is increased in idiopathic glomerulonephritis. Kidney Int 81:190–195

    Article  Google Scholar 

Download references

Acknowledgement

This study was supported by the funds from Primary Research & Development plan of Zhejiang Province (2020C03034) to Fei Han, Zhejiang Medical and Health Science and Technology Project (2019RC036) to Lan Lan and Project of Natural Science Foundation of Zhejiang Province (Q19H050030) to Pingping Ren.

Author information

Author notes

  1. Qiankun Zhang and Yin Wang have contributed equally to this work.

Authors and Affiliations

  1. Kidney Disease Center, The First Affiliated Hospital, College of Medicine, Key Laboratory of Kidney Disease Prevention and Control Technology, Institute of Nephrology, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang Province, People’s Republic of China

    Qiankun Zhang, Yin Wang, Yanhong Ma, Lan Lan, Pingping Ren, Jianghua Chen & Fei Han

  2. Division of Nephrology, Lishui Central Hospital, Lishui Hospital of Zhejiang University, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000, People’s Republic of China

    Qiankun Zhang, Yin Wang & Lie Jin

Authors
  1. Qiankun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanhong Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lie Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lan Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pingping Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jianghua Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fei Han
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fei Han.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Q., Wang, Y., Ma, Y. et al. The correlation of anti-β2-glycoprotein I antibody with complement activation in patients with idiopathic membranous nephropathy. Int Urol Nephrol 53, 955–959 (2021). https://doi.org/10.1007/s11255-020-02698-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11255-020-02698-2

Keywords

Search

Navigation