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The effect of thermochemotherapy with mitomycin C on normal bladder urothelium, an experimental study

  • Urology - Original Paper
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Abstract

Purpose

To investigate the effects of thermochemotherapy with mitomycin C (MMC) on normal rabbit bladder urothelium and to compare it with standard intravesical MMC and hyperthermia with normal saline.

Methods

Twenty-four male New Zealand rabbits, with a mean weight of 2.7 kg (in weight of 2.1–4.3 kg), were divided into three groups, each containing eight rabbits. Thermotherapy with only normal saline was performed in the first group, standard intravesical MMC was performed in the second group, and thermotherapy with MMC was performed in the last group. A week after the primary procedure, total cystectomy was performed and tissue samples were evaluated.

Results

The presence of epithelial vacuolar degeneration (p = 0.001), epithelial hyperplasia (p = 0.000), subepithelial fibrosis (p = 0.001) and hemorrhagic areas in the connective tissue (p = 0.002) was observed statistically significantly higher in the standard MMC group than in thermotherapy with normal saline group. There was almost a significant difference among standard MMC and normal saline group in terms of vascular congestion in the connective tissue (p = 0.08). Presence of epithelial vacuolar degeneration (p = 0.002), epithelial hyperplasia (p = 0.002), subepithelial fibrosis (p = 0.030), hemorrhagic areas (p = 0.011) and vascular congestion (p = 0.36) in the connective tissue was observed statistically significantly higher in the thermochemotherapy with MMC group than in standard intravesical MMC group. Polymorphonuclear cell infiltration was not considerable in any of the groups, and there was no significant difference between each groups (p = 0.140).

Conclusion

Administration of intravesical MMC causes a toxic effect on the normal urothelium of the bladder rather than an inflammatory reaction. Heating MMC significantly increased this effect.

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Acknowledgments

This project was supported by the Academic Research Project unit of the Süleyman Demirel University.

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Authors and Affiliations

  1. Department of Urology, Faculty of Medicine, Süleyman Demirel University, 32260, Isparta, Turkey

    Murat Uçar, Muammer Altok, Mehmet Umul, Mustafa Güneş, Tahsin Çapkin & Sedat Soyupek

  2. Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, 32260, Isparta, Turkey

    Dilek Bayram & İlkay Armağan

Authors
  1. Murat Uçar
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Correspondence to Muammer Altok.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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This article does not contain any studies with human participants. Informed consent was not obtained.

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Uçar, M., Altok, M., Umul, M. et al. The effect of thermochemotherapy with mitomycin C on normal bladder urothelium, an experimental study. Int Urol Nephrol 48, 79–84 (2016). https://doi.org/10.1007/s11255-015-1139-1

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  • DOI: https://doi.org/10.1007/s11255-015-1139-1

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