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Comparative therapeutic efficacy of oral administration of gluconeogenic precursors with nicotinamide and intravenous hypertonic dextrose solution for management of subclinical ketosis in Chilika buffaloes

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Abstract

Ketosis in dairy animals originates from negative energy status, associated increased absorption, and production of ketone bodies exceeding their use by the ruminants as an energy source. The present therapeutic experiment was carried out in 29 herds of Chilika buffaloes in 16 villages of three adjoining districts of Chilika Lake, Puri, Khurda, and Ganjam. Twenty Chilika buffaloes, detected positive for subclinical ketosis, were randomly selected for the study and divided into 2 groups, groups II and III, and were treated with hypertonic dextrose solution intravenously or gluconeogenic precursors along with nicotinamide orally, along with other supportive drugs in both the groups. Ten lactating Chilika buffaloes with no signs of ketosis and detected negative on Rothera test were included in the study as healthy controls (group I). Blood and milk samples were collected from all the 30 recruited buffaloes on days 0 (pre-treatment), 7, 14, and 28 for haematological and biochemical analysis. The subclinical ketosis in Chilika buffaloes did not have overt clinical signs. However, close examination revealed gradual drop in milk yield (100%), inappetence (59%), debility (46%), and uncoordinated gait (10%) without excitatory nervous signs. On day 7 following treatment, blood glucose concentration increased significantly. The mean serum triglyceride concentration of group III, treated with gluconeogenic precursors with nicotinamide, continued to decline significantly on subsequent observations. The serum enzyme activity, indicating status of liver function, declined following treatment in both the therapeutic groups. The intravenous administration of hypertonic dextrose solution compared to use of oral gluconeogenic precursors along with nicotinamide efficiently restored recovery from the subclinical ketosis in Chilika buffaloes.

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Data generated from the present study has been used in this manuscript.

References

  • Adler, J.H., Roberts, S.J. and Steel, R.G.D., 1957. The relation between reactions to Ross test on milk and urine and degree of ketonemia in dairy cows, Cornell Veterinarian, 47, 101-111

    CAS  PubMed  Google Scholar 

  • Constable, P.D., Hinchcliff, K.W., Done, S.H. and Grunberg, W., 2017. Metabolic diseases of Ruminants, In: Veterinary Medicine, 11thEdn Elsevier Ltd., St Louis, Missouri, pp 1662–1726

  • Dann, H. M., Drackley, J. K., Douglas, G. N., Janovick Guretzky, N. A., Litherland, N. B., Underwood, J. P., Loor, J. J., 2005. Physiological and pathological adaptations in dairy cows that may increase susceptibility to periparturient diseases and disorders. Italian Journal of Animal Science, 4, 323–344.

    Article  Google Scholar 

  • Dar, K.H., Ansari, M.M., Tantary, H.A., Baba, M.A. and Ashraf, I., 2014. Studies on efficacy of therapeutic protocols for ketosis in dairy cows, International Journal of Veterinary Science, 3 (3), 147-150

    Google Scholar 

  • Djokovic R., Ilic Z., Kurcubic V., Petrovic M., Cincovic M., Petrovic P.M., Caro-Petrovic V. (2019): Diagnosis of subclinical ketosis in dairy cows. Biotechnology in Animal Husbandry, 35 (2), 111-125. https://doi.org/10.2298/BAH1901001P

    Article  Google Scholar 

  • Doxy, D. L., 1983. Metabolic disorders: In Clinical Pathology and Diagnostic Procedures, 2nd Ed, Bailliiere Tindall, London, pp195-210.

    Google Scholar 

  • Fox, F. H., 1971. Clinical diagnosis and treatment of ketosis. Journal of Dairy Science 54, (6), 974-978

    Article  CAS  PubMed  Google Scholar 

  • Ghanem, M. M. and El-Deeb, W. M., 2010. Lecithin cholesterol acyl transferase (LCAT) activity as a predictor for ketosis and parturient haemoglobinuria in Egyptian water buffaloes. Research in Veterinary Science, 88, 20–25.

    Article  CAS  PubMed  Google Scholar 

  • Grohn, Y., Lindberg L. A, Bruss, M.L. and Farver, T. D., 1983. Fatty infiltration of liver in spontaneously ketotic dairy cows, Journal of Dairy Science, 66, 2320-2328

    Article  CAS  PubMed  Google Scholar 

  • Herdt, T. H., 2000. Ruminant adaptation to negative energy balance - influences on the etiology of ketosis and fatty liver. Veterinary Clinics North American Food Animal Practice, 16, 215–230

    Article  CAS  Google Scholar 

  • Kumar, A., Sindhu, N., Kumar, P., Kumar, T., Charaya, G. S., Jain, V., Sridhar, K., 2015. Incidence and clinical vital parameters in primary ketosis of Murrah buffaloes, Veterinary World 8(9), 1083-1087.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lean, I.J., Bruss, M.L., Troutt, H.F., Galland, J. C., Farver, T.B., Rostami, J., Holmberg, C.A. and Weaver, L.D., 1994. Bovine ketosis and somatotropin: Risk factors for ketosis and effects of ketosis on health and production. Research in. Veterinary Science, 57, 200-209

    Article  CAS  PubMed  Google Scholar 

  • Marutsova, V., Binev, R. and Marutsov, P., 2015. Comparative clinical and haematological investigations in lactating cows with subclinical and clinical ketosis, Mac Vet Rev, 38(2), 1-7

    Article  Google Scholar 

  • Mohamed, A. Youssef, Sabry Ahmed El-Khodery, Wael M. El-deeb, Waleed, E. E., and Abou, El-Amaiem (2010) Ketosis in buffalo (Bubalus bubalis) clinical findings and the associated oxidative stress level. Tropical Animal Health and Production 42,1771–1777

  • Patel, P. A., Patel, Y. G., Modi, R. J., and Trivedi, M. M., 2019. Study on housing practices followed by dairy animal owners in Anand district of middle Gujurat Region. Indian Journal of Veterinary Science and Biotechology 15 (2), 6-9

    Article  Google Scholar 

  • Rautmare, S.S. and Anantwar, L.G., 1993. Prevalence, biochemistry and treatment of clinical ketosis in buffalo (Bubalus bubails), Indian Journal of Veterinary Medicine, 12(9), 28

    Google Scholar 

  • Reddy, Y.R. and Chakravarthi, M. K., 2015. Housing of dairy animals under Indian conditions. Dairy year book 2014-15, 117-121

    Google Scholar 

  • Sahoo, S.S., Patra, R. C., Behera, P. C. and Swarup, D., 2009. Oxidative stress indices in the erythrocytes from lactating cows after treatment for subclinical ketosis with antioxidant incorporated in the therapeutic regime. Veterinary Research Communication, 33, 281-290

    Article  CAS  Google Scholar 

  • Schlumbohm, C. and Harmeyer, J., 2003. Hypocalcemia reduces endogenous glucose production in hyperketonemic sheep, Journal of Dairy Science, 86(6), 1953-1962.

    Article  CAS  PubMed  Google Scholar 

  • Sharma, N., 2010. Economically important production diseases of dairy animals, SMVS’ Dairy year book, 47–65.

  • Siddiqui, M.F., Waghmare, S.P., Mode, S.G., Pajai, K.S., Adarsh, M. S., Ravikanth, K., 2014. An herbal tool- AV/KPC/10 to check the sub clinical ketosis in cows, The Journal of Veterinary Science Photon, 116, 457-464

    Google Scholar 

  • Taylor, E.J., 1994. Dorland’s Illustrated Medical Dictionary, 28th ed. W.B. Saunders, Philadelphia, USA.

    Google Scholar 

  • Teli, S.A. and Ali, S.L, 2007. Plasma glucose and insulin profiles in ketotic buffaloes, Online Veterinary Scan, 2(1), 19

    Google Scholar 

  • Thammacharoen, S., Semsirmboon, S., Chanpongsang, S., Chaiyabutr, N., Panyasomboonying, P., Khundamrongkul, P., Puchongmart, P., Wichachai, W. (2021) Seasonal effect of milk yield and blood metabolites in relation to ketosis of dairy cows fed under a high ambient temperature, Veterinary World, 14(9), 2392-2396.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Turk, R., Juretić, D., Geres, D., Svetina, A., Turk, N., Flegar-Mestrić, Z. (2008) Influence of oxidative stress and metabolic adaptation on PON1 activity and MDA level in transition dairy cows. Animal Reproduction Science, 108, 98 –106

    Article  CAS  PubMed  Google Scholar 

  • Vaja, V.B., Manvar, N.B., Dodiya, P.G., Patel, J.S., Joseph, J.P. and Patel, B.R., 2016. Clinico-therapeutic management of ketosis in Gir cattle, International Journal of Science, 5(5), 2278-2287

    Google Scholar 

  • Weiss, D.J. and Wardrop, K.J., 2010. Schalm’s Veterinary Hematology, 6th Edn., Wiley Blackwell, State Avenue, Ames, Iowa, USA

    Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, Odisha, India

    Ashish Kumar Dora, S. K. Senapati, R. C. Patra & G. R. Jena

  2. Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751003, India

    P. K. Rath

  3. Kamala Nehru Women’s College, Rama Devi Women’s University, Bhubaneswar, 751003, India

    Rajasri Sahoo

  4. Department of Preventive Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar, 751009, India

    Sangram Biswal

Authors
  1. Ashish Kumar Dora
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  2. S. K. Senapati
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  3. R. C. Patra
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  4. P. K. Rath
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  5. Rajasri Sahoo
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  6. Sangram Biswal
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  7. G. R. Jena
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Contributions

Author contributions

1 Ashish Kumar Dora Conducted the experiments, analysed the data, 2 S.K Senapati, Planned and designed the experiments,3 R.C Patra Supervised the experiments,4 P. K Rath Co-supervised the experiments, 5 Sahoo, Rajasri Wrote the manuscript,6 Sangram Biswal Co-supervised the experiments, 7 Jena, G.R Co-supervised the experiments

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Correspondence to R. C. Patra.

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Dora, A.K., Senapati, S.K., Patra, R.C. et al. Comparative therapeutic efficacy of oral administration of gluconeogenic precursors with nicotinamide and intravenous hypertonic dextrose solution for management of subclinical ketosis in Chilika buffaloes. Trop Anim Health Prod 55, 326 (2023). https://doi.org/10.1007/s11250-023-03738-6

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