Abstract
Bovine tuberculosis (BTB) is a widespread zoonosis in developing countries but has received little attention in many sub-Saharan African countries including Sudan and particularly in some parts such as Darfur states. This study aimed to detect bovine tuberculosis among caseous materials of cattle slaughtered in abattoirs in South Darfur State, Sudan by using microscopic and PCR-based methods. The study was a cross-sectional abattoir-based study which examined a total of 6,680 bovine carcasses for caseous lesions in South Darfur State between 2007 and 2009. Collected specimens were examined for the presence of acid-fast bacilli (AFB) by using microscopic and culture techniques. Isolated mycobacteria were identified by selected conventional cultural and biochemical tests in comparison to a single tube multiplex PCR (m-PCR) assay which detect Mycobacterium bovis-specific 168-bp amplicons. Of the total 6,680 slaughtered cattle examined in South Darfur, 400 (6 %) showed caseations restricted to lymph nodes (86.8 %) or generalized (13.2 %). Bovine tuberculosis was diagnosed in 12 (0.18 %), bovine farcy in 59 (0.88 %), unidentified mycobacteria in 6 (0.09 %), and missed or contaminated cultures in 7 (0.1 %). Out of 18 cultures with nonbranching acid-fast rods, 12 amplified unique 168-bp sequence specific for M. bovis and subsequently confirmed as M. bovis. With the exception of the reference M. tuberculosis strains, none of the remaining AFB amplified the 337-bp amplicon specific for M. tuberculosis. It could be concluded that bovine tuberculosis is prevalent among cattle in South Darfur representing 4.5 % from all slaughtered cattle with caseous lesions. The study sustains microscopy as a useful and accessible technique for detecting AFB. m-PCR assay proved to be valuable for confirmation of BTB and its differentiation from other related mycobacteriosis, notably bovine farcy.
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References
Awad F. I., 1962. Studies on Type-Determination and Epidemiology of Tuberculosis among cattle in Sudan. Zentralblatt für Veterinärmedizin, 9, 890–898
Awad F. I., Karib A. A. and Fawi M. T., 1959. Some observations on tuberculosis among cattle in the Sudan. Zentralblatt für Veterinärmedizin, 6, 180–184
Ayele W. Y., Neill S. D., Zinsstag J., Weiss M. G., Pavlik I., 2004. Bovine tuberculosis: an old disease but a new threat to Africa. International Journal of Tuberculosis and Lung Diseases, 8, 924–937
Bakashi, C.S., Shah, D.H., Verma, R., Singh, R.K. and Malik, M., 2005. Rapid differentiation of Mycobacterium bovis and Mycobacterium tuberculosis based on a 12.7-kb fragment by a single tube multiplex-PCR. Veterinary Microbiology, 109; 211–216
Boukary, A.R., Thys, E., Abatih, E., Gamatié, D., Ango, I., Yenikoye, A., Saegerman, C., 2011. Bovine tuberculosis prevalence survey on cattle in the rural livestock system of Torodi (Niger). PLoS One, 6, e24629
Cook, V. J., C. Y. Turenne, J. Wolfe, R. Pauls, and A. Kabani., 2003. Conventional methods versus 16S ribosomal DNA sequencing for identification of nontuberculous mycobacteria: cost analysis. Journal of Clinical Microbiology, 41, 1010-1015
Corner, L.A., 1994. Post mortem diagnosis of Mycobacterium bovis infection in cattle. Veterinary Microbiology, 40, 53-63
Cosivi. O., Grange, J.M., Daborn, C.J., Raviglione, M.C., Fujikura, T., et al., 1998. Zoonotic tuberculosis due to Mycobacterium bovis in developing countries. Emerging Infectious Diseases, 4, 59–70
Cousins, D.V., 2001. Mycobacterium bovis infection and control in domestic livestock. Revue Scientifique et Technique, 20, 71-85
Diguimbaye-Djaibé, C., Hilty, M., Ngandolo, R., Mahamat, H.H., Pfyffer, G.E., Baggi, F., Hewinson, G., Tanner, M., Zinsstag, J., Schelling, E., 2006. Mycobacterium bovis isolates from tuberculous lesions in Chadian zebu carcasses. Emerging Infectious Diseases, 12, 769-771
Gumi, B., Schelling, E., Firdessa, R., Aseffa, A., Tschopp, R., Yamuah, L., Young, D., Zinsstag, J.2011. Prevalence of bovine tuberculosis in pastoral cattle herds in the Oromia region, southern Ethiopia. Tropical Animal Health and Production, 43, 1081-1087
Haddad, N., Masselot, M., Durand, B., 2004. Molecular differentiation of Mycobacterium bovis isolates. Review of main techniques and applications. Research in Veterinary Science, 76, 1-18
Hamid, M.E., 2012. Epidemiology, pathology, immunology and diagnosis of bovine farcy: a review. Preventive Veterinary Medicine, 105, 1-9
Isenberg, H.D., ed. 1998. Essentials Procedures for Clinical Microbiology. Washington DC: ASM Press, pp.171–359
Kent, P.T. and Kubica, G.P., 1985. Public health Mycobacteriology: Guide for the Level III Laboratory. Centers for Disease Control, US Department of Health and Human Services, Atlanta, GA. Pp. 31–70
Müller, B., Vounatsou, P., Ngandolo, B.N., Diguimbaye-Djaïbe, C., Schiller, I., Marg-Haufe, B., Oesch, B., Schelling, E., Zinsstag, J., 2009. Bayesian receiver operating characteristic estimation of multiple tests for diagnosis of bovine tuberculosis in Chadian cattle. PLoS One, 4, e8215
Office de International Epidemiologues (OIE)., 2009.Terrestrial Manual of Bovine tuberculosis. Pp. 1–16
Osman, A.B., 2007. Caseation in lymph nodes of slaughtered cattle with a special reference to bovine tuberculosis. Veterinary Medical Journal, Giza, 55, 1101-1109
Rasolofo Razanamparany, V., Quirin, R., Rapaoliarijaona, A., Rakotoaritahina, H., Vololonirina, E.J., Rasolonavalona, T., Ferdinand, S., Sola, C., Rastogi, N., Ramarokoto, H., Chanteau, S., 2006. Usefulness of restriction fragment length polymorphism and spoligotyping for epidemiological studies of Mycobacterium bovis in Madagascar: description of new genotypes. Veterinary Microbiology, 114, 115-122
Sulieman, M.S., Hamid, M.E., 2002. Identification of acid fast bacteria from caseous infections among slaughtered cattle in Sudan. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health. 49, 415-418
Tschopp, R., Aseffa, A., Schelling, E., Berg, S., Hailu, E., Gadisa, E., Habtamu, M., Argaw, K., Zinsstag, J., 2010. Bovine tuberculosis at the wildlife-livestock-human interface in Hamer Woreda, South Omo, Southern Ethiopia. PLoS One, 5, e12205
Vincent, V., Brown-Elliot. B., Jost. K.C., and Wallace, R.J., 2009. Mycobacterium: Phenotypic and genotypic identification. Manual of Clinical Microbiology, 8th edition, pp. 560–584
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The authors thank Dr. Manal Hassan Ali of the Central Veterinary Research Laboratory, Soba, Sudan; and the staff of the Tuberculosis Unit, National Health Laboratory, Khartoum, Sudan for their help.
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Asil, E.T.A., El Sanousi, S.M., Gameel, A. et al. Bovine tuberculosis in South Darfur State, Sudan: an abattoir study based on microscopy and molecular detection methods. Trop Anim Health Prod 45, 469–472 (2013). https://doi.org/10.1007/s11250-012-0241-y
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DOI: https://doi.org/10.1007/s11250-012-0241-y