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Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm

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Abstract

The CDKA gene is linked to the cellular control. This gene was isolated from coconut palm (Cocos nucifera L) and a detailed expression analysis was done during somatic embryogenesis. Analysis of the deduced amino acid sequence showed the most important residues to be conserved. The highest homology was with Picea abies (96% similarity). Expression of the putative CnCDKA gene steadily increased during embryogenic callus formation phase when embryogenic competence is attained. In situ hybridization specified the localization of the transcripts as being mainly in a few cell layers within the meristematic centres in embryogenic calli at 90 day cultures. Analysis of CnCDKA expression at different somatic embryo formation stages showed that the expression was decreased progressively with the lowest expression in germinated somatic embryos.

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Acknowledgments

L. Saenz received a postdoctoral fellowship from the Université Montpellier 2 (France) and continued support from the Centro de Investigación Científica de Yucatán (CICY). The authors also thank J-L Chan for assistance with the in vitro culture. This research was partially funded by CONACYT, México (Grant no. 43834-Z) and a scholarship awarded M. Montero (no. 183253).

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Authors and Affiliations

  1. Biotechnology Unit, Centro de Investigación Científica de Yucatán, Calle 43 No. 130. Colonia Chuburna de Hidalgo, 97200, Mérida, Yucatán, México

    Mayra Montero-Cortés, Francisco Rodríguez-Paredes, Caroline Burgeff, Teresa Pérez-Nuñez, Iván Córdova, Carlos Oropeza & Luis Sáenz

  2. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier Cedex 5, France

    Jean-Luc Verdeil

  3. Institut de Recherche pour le Développement, Montpellier Cedex, France

    Jean-Luc Verdeil

Authors
  1. Mayra Montero-Cortés
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  2. Francisco Rodríguez-Paredes
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  3. Caroline Burgeff
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  4. Teresa Pérez-Nuñez
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  5. Iván Córdova
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  6. Carlos Oropeza
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  7. Jean-Luc Verdeil
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  8. Luis Sáenz
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Correspondence to Luis Sáenz.

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Montero-Cortés, M., Rodríguez-Paredes, F., Burgeff, C. et al. Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm. Plant Cell Tiss Organ Cult 102, 251–258 (2010). https://doi.org/10.1007/s11240-010-9714-8

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  • DOI: https://doi.org/10.1007/s11240-010-9714-8

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