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Obtaining autotetraploids in vitro at a high frequency in Citrus sinensis

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Abstract

Tetraploid plants are essential for interploid hybridization to create triploid seedless citrus. Here we report a simple and efficient in vitro method for generating autotetraploids for sweet orange (Citrus sinensis). Cell-division activity in ‘Anliucheng’ sweet orange callus was analyzed using flow cytometry to determine the peak frequency of cell division at which time callus in a liquid media and solid media was treated with 1000 mg l−1 colchicine. The percentage of the DNA-content-varied cells in the callus increased markedly from 11.0% to 44.4% and to 59.0% for liquid and solid media respectively. A total of 20 tetraploid plantlets were recovered via embryogenesis from 47 plantlets regenerated from the treated callus. All the autotetraploids were derived from different embryoids. Autotetraploids will be useful parents for interploid hybridization to generate commercially valuable seedless triploid citrus cultivars.

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Abbreviations

BA:

6-Benzylaminopurine

KT:

Kinetin

MT:

Murashige and Tucker (1969)

NAA:

α−Naphthalene acetic acid

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Acknowledgements

We thank our colleagues from the National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University for their support and suggestions. This work was supported by the National Natural Science Foundation of China (No. 30570973) and IRT project of MOE of China (No. 0548). We appreciated the critical review of the manuscript by Dr. Joseph L. Smilanick of USDA in Fresno CA.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, Hubei, China

    June Zhang, Min Zhang & Xiuxin Deng

  2. Key Laboratory of Ministry of Education for Plant Development Biology, College of Life Science, Wuhan University, 430072, Wuhan, Hubei, China

    June Zhang

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  1. June Zhang
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  2. Min Zhang
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  3. Xiuxin Deng
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Correspondence to Xiuxin Deng.

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Zhang, J., Zhang, M. & Deng, X. Obtaining autotetraploids in vitro at a high frequency in Citrus sinensis . Plant Cell Tiss Organ Cult 89, 211–216 (2007). https://doi.org/10.1007/s11240-007-9240-5

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  • DOI: https://doi.org/10.1007/s11240-007-9240-5

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