Skip to main content
SpringerLink
Log in

In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays)

  • Research note
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Haploid induction has potential application for maize breeding. This paper reports that maize haploid plants have been induced by in vitro culture of pollinated ovaries. From a total of 26,400 cultured ovaries, 24 haploid plants were obtained and two of them were doubled after colchicine treatment. The maximum frequency of gynogenesis was 0.17% at 19.5 h post-pollination (HPP). The results showed that HPP was an important factor affecting plant induction from ovaries. Regenerated diploid R0 plants were then subjected to genetic analysis using SSR molecular markers. One R0 plant, whose progeny revealed a high level of homogeneity for several agro-morphological traits, was homozygous at 20 loci tested, with 11 showing paternal and 9 maternal banding pattern. This demonstrates that it is feasible to induce maize haploid plants by in vitro culture of pollinated ovaries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CTAB:

cetyltrimethyl ammonium bromide

DMSO:

dimethyl sulphoxide

2.4-D :

2,4-dichlorophenoxyacetic acid

HPP:

the number of hours post pollination

Kin:

kinetin

NAA:

α-naphthaleneacetic acid

SSR:

simple sequence repeats

Reference

  • Ao GM, Zhao ShX, Li GH, (1982) In vitro induction of haploid plantlets from unpollinated ovares of corn (Zea mays L.) Acta Genetica Sinica 9(4): 281–283

    Google Scholar 

  • Alatortseva TA, Tyrnov VS, (2001) Reproducing of haploid and diploid maize forms in vitro Maize Genet. Coop News. Lett. 75:56

    Google Scholar 

  • Barloy D, Beckert M, (1993) Improvement of regeneration ability of androgenetic embryos by early anther transfer in maize Plant Cell Tiss. Org. Cult. 33:45–50

    Article  Google Scholar 

  • Beckert M, (1994) Advantages and disadvantages of the use of in vitro/in situ produced DH maize plants. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry vol 25 Maize. Springer-Verlag, Berlin Heidelberg, pp 201–213

    Google Scholar 

  • Coe EH, (1957) High haploid line Maize Genet. Coop. News Lett. 31:138–139

    Google Scholar 

  • Chu CC, Wang CC, Sun CS, Hsu C, Yin DC, Chu CY, (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen source Sci. Sinica 18:659–668

    Google Scholar 

  • Doctrinal M, Sangwan RS, Sangwan-Norreel BS, (1989) In vitro gynogenesis in Beta vulgaris L.: Effects of plant growth regulators, temperature, genotypes and season Plant Cell Tiss. Org. Cult. 17:1–12

    Google Scholar 

  • Dore C, Marie F, (1993) Production of gynogenetic plants of onion (Allium cepa L.) after crossing with irradiated pollen Plant breeding 111:142–147

    Article  Google Scholar 

  • Dufour P, Johnsson C, Antoine-Michard S, Cheng R, Murigneux A, Beckert M, (2001) Segregation distortion at marker loci: Variation during microspore embryogenesis in maize Theor. Appl. Genet 102:993–1001

    Article  CAS  Google Scholar 

  • Eder J, Chalyk S, (2002) In vivo haploid induction in maize Theor. Appl. Genet. 104:703–708

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F, (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture Physiol. Plant. 15:473–479

    Article  CAS  Google Scholar 

  • Przyborowski J, Niemirowicz-Szczytt K, (1994) Main factors affecting cucumber (Cucumis satyivus L.) haploid embryo development and haploid plant characteristic Plant Breeding 112:70–75

    Article  Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgenson RA, Allard RW, (1984) Ribosomal DNA spacer length polymorphism inbarley: Mendelian inheritance,Chromosomal location and population dynamics Proc. Natl. Acad. Sci. USA 81:8014–8018

    Article  PubMed  CAS  Google Scholar 

  • Tosca A, Arcara L, Frangi P, (1999) Effect of genotype and season on gynogenesis efficiency in Gerbera Plant Cell Tiss. Org. Cult. 59:77–80

    Article  Google Scholar 

Download references

Acknowledgements

The authors are grateful to professor Ouyang Junwen for his critical reading of the manuscript.

Author information

Authors and Affiliations

  1. State Key Laboratory of Agrobiotechnology and National Center for Maize Improvement, China Agricultural University, Beijing , 100094, China

    Feiyu Tang, Yazhong Tao, Tianyong Zhao & Guoying Wang

Authors
  1. Feiyu Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yazhong Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tianyong Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guoying Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Guoying Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tang, F., Tao, Y., Zhao, T. et al. In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays). Plant Cell Tiss Organ Cult 84, 233–237 (2006). https://doi.org/10.1007/s11240-005-9017-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-005-9017-7

Keywords

Search

Navigation