Skip to main content
SpringerLink
Log in

Exosome mediated differentiation of megakaryocytes: a study on TLR mediated effects

  • Letter to the Editor
  • Published:
Journal of Thrombosis and Thrombolysis Aims and scope Submit manuscript

Abstract

Megakaryocytes are large polyploid bone marrow cells whose function is to produce circulatory platelets. Megakaryocytes are also known to release extracellular vesicles (EVs) of varying sizes. Toll like receptors (TLRs), present on the sentinel cells are essential components of the innate immune response, these receptors are also expressed by platelets and megakaryocytes. Our data provide the evidence that TLR-2 induced MKEVs are able to recapitulate TLR-2 signalling in megakaryocytic cell line (Dami cells) and that likely induces megakaryocytic maturation by increasing the production of cytokines involved in MK maturation. TLR-2 induced MKEVs may be involved in replenishment of the immune effector platelets in circulation and its progenitor megakaryocyte in bone marrow for the physiological need of the platelets by inducing the maturation of megakaryocyte.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Paschon V, Takada SH, Ikebara JM, Sousa E, Raeisossadati R, Ulrich H, Kihara AH (2016) Interplay between exosomes, microRNAs and toll-like receptors in brain disorders. Mol Neurobiol 53(3):2016–2028

    Article  CAS  PubMed  Google Scholar 

  2. Tao SC, Guo SC, Zhang CQ (2017) Platelet-derived extracellular vesicles: an emerging therapeutic approach. Int J Biol Sci 13(7):828–834

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Butler JT, Abdelhamed S, Kurre P (2018) Extracellular vesicles in the hematopoietic microenvironment. Haematologica 103(3):382–394

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Jiang J, Kao CY, Papoutsakis ET (2017) How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells? J Controll Release 10(247):1–18

    Article  CAS  Google Scholar 

  5. Srinivasan S, Su M, Ravishankar S et al (2017) TLR-exosomes exhibit distinct kinetics and effector function. Sci Rep 7:41623

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Jayachandran M, Brunn GJ, Karnicki K, Miller RS, Owen WG, Miller VM (2007) In vivo effects of lipopolysaccharide and TLR4 on platelet production and activity: implications for thrombotic risk. J Appl Physiol 102(1):429–433

    Article  CAS  PubMed  Google Scholar 

  7. Undi RB, Sarvothaman S, Narasaiah K, Gutti U, Gutti RK (2016) Toll-like receptor 2 signalling: significance in megakaryocyte development through wnt signalling cross-talk and cytokine induction. Cytokine 83:245–249

    Article  CAS  PubMed  Google Scholar 

  8. Flaumenhaft R, Dilks JR, Richardson J, Alden E, Patel-Hett SR, Battinelli E, Klement GL, Sola-Visner M, Italiano JE Jr (2009) Megakaryocyte-derived micro particles: direct visualization and distinction from platelet-derived microparticles. Blood 113(5):1112–1121

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by Department of Biotechnology (DBT), Department of Science and Technology (DST, Grant No. EEQ/2018/000853), Indian Council of Medical Research (ICMR) and University Grants Commission (UGC-SAP-DRS, UPE-2) grants of Government of India. We appreciate the funding in form of Council of Scientific and Industrial Research (CSIR) and UGC Fellowships from Government of India.

Author information

Authors and Affiliations

  1. Department of Biochemistry, School of Life Sciences, University of Hyderabad, (PO) Gachibowli, Hyderabad, TS, 500046, India

    Narasaiah Kovuru, Sanjeev Raghuwanshi & Ravi Kumar Gutti

Authors
  1. Narasaiah Kovuru
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sanjeev Raghuwanshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ravi Kumar Gutti
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

N.K. designed and performed experiments, and prepared the manuscript. S.R. prepared the manuscript and R.G. designed and supervised the study.

Corresponding author

Correspondence to Ravi Kumar Gutti.

Ethics declarations

Conflicts of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kovuru, N., Raghuwanshi, S. & Gutti, R.K. Exosome mediated differentiation of megakaryocytes: a study on TLR mediated effects. J Thromb Thrombolysis 48, 171–173 (2019). https://doi.org/10.1007/s11239-019-01862-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11239-019-01862-5

Keywords

Search

Navigation