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Association of small CAB-like proteins (SCPs) of Synechocystis sp. PCC 6803 with Photosystem II

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Abstract

The cyanobacterial small CAB-like proteins (SCPs) are one-helix proteins with compelling similarity to the first and third transmembrane helix of proteins belonging to the CAB family of light-harvesting complex proteins in plants. The SCP proteins are transiently expressed at high light intensity and other stress conditions but their exact function remains largely unknown. Recently we showed association of ScpD with light-stressed, monomeric Photosystem II in Synechocystis sp. PCC 6803 (Yao et al. J Biol Chem 282:267–276, 2007). Here we show that ScpB associates with Photosystem II at normal growth conditions. Moreover, upon introduction of a construct into Synechocystis so that ScpB is expressed continuously under normal growth conditions, ScpE was detected under non-stressed conditions as well, and was copurified with tagged ScpB and Photosystem II. We also report on a one-helix protein, Slr1544, that is somewhat similar to the SCPs and whose gene is cotranscribed with that of ScpD; Slr1544 is another member of the extended light-harvesting-like (Lil) protein family, and we propose to name it LilA.

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Abbreviations

CAB:

Chlorophyll a/b binding

ELIP:

Early-light induced protein

HLIP:

High-light induced protein

LHC:

Light-harvesting complex

Lil:

Light-harvesting like

PAGE:

Polyacrylamide gel electrophoresis

PS:

Photosystem

SCP:

Small CAB-like protein

LC-MS/MS:

Liquid chromatography combined with tandem mass spectrometry

β-dM:

β-d-maltoside

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Acknowledgments

The authors would like to thank the Swedish Foundation for International Cooperation in Research and Higher Education (STINT) for supporting the collaboration between the two research groups. C.F. is very grateful to the Royal Swedish Academy of Sciences (KVA) for granting her research position and to the Swedish Research Council (VR) for financial support. W.V. acknowledges research grant support from the US Department of Energy (DE-FG02-04ER15543).

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Authors and Affiliations

  1. Department of Chemistry, Umeå University, Umeå, 901 87, Sweden

    Galyna Kufryk, Miguel A. Hernandez-Prieto, Thomas Kieselbach, Helder Miranda & Christiane Funk

  2. School of Life Sciences and Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ, 85287-4501, USA

    Galyna Kufryk, Miguel A. Hernandez-Prieto, Helder Miranda & Wim Vermaas

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  1. Galyna Kufryk
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  2. Miguel A. Hernandez-Prieto
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  3. Thomas Kieselbach
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  4. Helder Miranda
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  5. Wim Vermaas
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  6. Christiane Funk
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Corresponding author

Correspondence to Christiane Funk.

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Galyna Kufryk and Miguel Hernandez-Prieto have contributed equally to this work.

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Kufryk, G., Hernandez-Prieto, M.A., Kieselbach, T. et al. Association of small CAB-like proteins (SCPs) of Synechocystis sp. PCC 6803 with Photosystem II. Photosynth Res 95, 135–145 (2008). https://doi.org/10.1007/s11120-007-9244-3

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