Abstract
The isiAB genes have proven to be highly stress-responsive under a variety of environmental conditions, including iron deficiency, high salt and oxidative stress. In order to understand the function of IsiA and its importance in oxidative stress, we constructed a knock out mutant of the isiA gene and compared differential gene expression of the ΔisiA strain in the presence and absence of H2O2. We used the full genome microarray for the cyanobacterium Synechocystis sp. PCC 6803 as previously described [Postier BL, Wang HL, Singh A, Impson L, Andrews, HL, Klahn J, Li H, Risinger G, Pesta D, Deyholos M, Galbraith DW, Sherman LA and Burnap RL (2003) BMC Genenomics 4: 23–34]. We determined that one of the main differences in ΔisiA compared to wild-type (in the absence of peroxide) was the induction of a gene cluster (sll1693–sll1696) that encoded genes resembling pilins or general secretory proteins (Gsp). These proteins are targeted to the cytoplasmic membrane and we suggest that they may be involved in the assembly of membrane complexes, including pigment–protein complexes. The ΔisiA strain was more resistant to H2O2 compared to the wild-type. In the presence of 1.5 mM H2O2 for 30 min, a cluster of genes that includes a peroxiredoxin was induced 7- to 8-fold and we suggest that this peroxide scavenging enzyme is responsible for the increased peroxide resistance of the ΔisiA strain.
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References
D Bhaya NR Bianco D Bryant AR Grossman (2000) ArticleTitleType IV pilus biogenesis and motility in the cyanobacterium Synechocystis sp. PCC 6803 Mol Microbiol 37 941–951 Occurrence Handle10972813
D Bhaya A Takahashi AR Grossman (2001) ArticleTitleLight regulation of type IV pilus-dependent motility by cheosensor-like elements in Synechocystis. PCC 6803 Proc Natl Acad Sci USA 98 7540–7545 Occurrence Handle11404477
TS Bibby J. Nield J Barber (2001) ArticleTitleIron deficiency induces the formation of an antenna ring around trimeric Photosystem I in cyanobacteria Nature 412 743–745 Occurrence Handle11507643
RL Burnap T Troyan LA Sherman (1993) ArticleTitleThe highly abundant chlorophyll–protein complex of iron-deficient Synechococcus sp. PCC7942 (CP43′) is encoded by the isiA gene Plant Physiol 103 893–902 Occurrence Handle8022940
KJ Dietz (2003) ArticleTitlePlant peroxiredoxins Annu Rev Plant Biol 54 93–107 Occurrence Handle14502986
BR. Green (2003) The evolution of light-harvesting antennas BR. Green WW. Parson (Eds) Light-Harvesting Antennas in Photosynthesis. Kluwer Academic Publishers Dordrecht The Netherlands 129–168
Q He W Vermaas (1999) ArticleTitleGenetic deletion of proteins resembling type IV pilins in Synechocystis sp. PCC 6803: their role in binding or transfer of newly synthesized chlorophyll Plant Mol Biol 39 1175–1188 Occurrence Handle10380804
M Kobayashi T Ishizuka M Katayama M Kanehisa M Bhattacharyya-Pakrasi HB Pakrasi M Ikeuchi (2004) ArticleTitleResponse to oxidative stress involves a novel peroxiredoxin gene in the unicellular cyanobacterium Synechocystis PCC 6803 Plant Cell Physiol 45 290–299 Occurrence Handle15047877
DE Laudenbach NA Straus (1988) ArticleTitleCharacterization of a cyanobacterial iron stress-induced gene similar to psbC J Bacteriol 170 5018–5026 Occurrence Handle3141374
H Li AK Singh LM McIntyre LA Sherman (2004) ArticleTitleDifferential gene expression in response to hydrogen peroxide and the putative PerR regulon of Synechocystis sp. PCC 6803 strain J Bacteriol 186 3331–3345 Occurrence Handle15150218
J Neter MH Kutner CJ. Nachtsheim W. Wasserman (1996) Applied Linear Statistical Models EditionNumber4 McGraw-Hill Boston 110–111
Y Nishiyama H Yamamoto SI Allakhverdiev M Inaba A Yokota N Murata (2001) ArticleTitleOxidative stress inhibits the repair of photodamage to the photosynthetic machinery EMBO J 20 5587–5594 Occurrence Handle11598002
D Nunn (1999) ArticleTitleBacterial type II protein export and pilus biogenesis: more than just homologies? Trends Cell Biol 9 402–408 Occurrence Handle10481178
HB Pakrasi HC Riethman LA Sherman (1985) ArticleTitleOrganization of pigment proteins in the Photosystem II complex of the cyanobacterium Anacystis nidulans R2 Proc Natl Acad Sci USA 82 6903–6907 Occurrence Handle3931080
BL Postier HL Wang A Singh L Impson HL Andrews J Klahn H Li G Risinger D Pesta M Deyholos DW Galbraith LA Sherman RL Burnap (2003) ArticleTitleThe construction and use of bacterial DNA microarrays based on an optimized two-stage PCR strategy BMC Genomics 4 23–34 Occurrence Handle12803655
G Regelsberger C Jakopitsch L Plasser H Schwaiger PG Furtmuller GA Peschek M. Zamocky C Obinger (2002) ArticleTitleOccurence and biochemistry of hydroperoxidases in oxygenic phototrophic prokaryotes (cyanobacteria) Plant Physiol Biochem 40 79–490 Occurrence Handle10.1016/S0981-9428(02)01405-5
S Sandstrom YI Park G Oquist P Gustafsson (2001) ArticleTitleCP43′, the isiA gene product, functions as an excitation energy dissipator in the cyanobacterium Synechococcus sp PCC 7942 Photochem Photobiol 74 431–437 Occurrence Handle11594057
TV Sergeyenko DA Los (2003) ArticleTitleCyanobacterial leader peptides for protein secretion FEMS Microbiol Lett 218 351–357 Occurrence Handle12586416
AK Singh LM McIntyre LA Sherman (2003) ArticleTitleMicroarray analysis of the genome-wide response to iron deficiency and iron reconstitution in the cyanobacterium Synechocystis sp PCC 6803 Plant Physiol 132 1825–1839 Occurrence Handle12913140
J Vinnemeier A Kunert M Hagemann (1998) ArticleTitleTranscriptional analysis of the isiAB operon in salt-stressed cells of the cyanobacterium Synechocystis sp PCC 6803 FEMS Microbiol Lett 169 323–330 Occurrence Handle9868777
N Yeremenko R Kouril JA Ihalainen S D’Haene N Oosterwijk Particlevan EG Andrizhiyevskaya W Keegstra HL Dekker M Hagemann EJ Boekema HC Matthijs JP Dekker (2004) ArticleTitleSupramolecular organization and dual function of the IsiA chlorophyll-binding protein in cyanobacteria Biochemistry 43 10308–10313 Occurrence Handle15301529
S Yoshihara X Geng S Okamoto K Yura T Murata M Go M Ohmori M Ikeuchi (2001) ArticleTitleMutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis PCC 6803 Plant Cell Physiol 42 63–73 Occurrence Handle11158445
N Yousef EK Pistorius KP Michel (2003) ArticleTitleComparative analysis of idiA and isiA transcription under iron starvation and oxidative stress in Synechococcus elongatus PCC 7942 wild-type and selected mutants Arch Microbiol 180 471–483 Occurrence Handle14605795
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Singh, A.K., Li, H., Bono, L. et al. Novel adaptive responses revealed by transcription profiling of a Synechocystis sp. PCC 6803 ΔisiA mutant in the presence and absence of hydrogen peroxide. Photosynth Res 84, 65–70 (2005). https://doi.org/10.1007/s11120-004-6429-x
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DOI: https://doi.org/10.1007/s11120-004-6429-x