Abstract
A previously reported cytoplasmic male sterility-associated fragment was detected in chili pepper (Capsicum annuum L.) and sequenced in the mitochondria of a sterile plant. This fragment, 16 kb in size, contains two common mitochondrial genes, coxII and atp6-2, and the newly detected gene orf456, which appears to be a strong candidate gene for the male sterile phenotype. The data show that one nucleotide (Cytosine) at the +449 position is missing compared with the original sequence data of orf456. By translation to the amino acid sequence, the termination codon was found 49 nt downstream from the original site. This hypothetical extension was confirmed by RT-PCR, which showed a sequence size of 507 bp. Since pollen fertility can be restored by a nuclear restorer of fertility gene (Rf), the transcription of orf507 was checked in four stages of flower development. The RT-PCR resulted in a fragment in stage III in the restorer line, indicating a possible role in microspore development.
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The authors thank Dr. Hidenori Sassa for his help in cloning the CMS-associated 16 kb fragment.
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Gulyas, G., Shin, Y., Kim, H. et al. Altered Transcript Reveals an Orf507 Sterility-Related Gene in Chili Pepper (Capsicum annuum L.). Plant Mol Biol Rep 28, 605–612 (2010). https://doi.org/10.1007/s11105-010-0182-4
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DOI: https://doi.org/10.1007/s11105-010-0182-4